A5011280934- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Immune Cell Function and Interaction
- Nematode management and characterization studies
- Immunodeficiency and Autoimmune Disorders
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive System and Pregnancy
- Autoimmune Bullous Skin Diseases
- Renal Diseases and Glomerulopathies
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Pancreatic function and diabetes
- Invertebrate Taxonomy and Ecology
- Pediatric Hepatobiliary Diseases and Treatments
- High Altitude and Hypoxia
- Cancer Immunotherapy and Biomarkers
- Phytoestrogen effects and research
- Plant Taxonomy and Phylogenetics
- Aluminum toxicity and tolerance in plants and animals
- Genetic diversity and population structure
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
BGI Genomics
2022
BGI Group (China)
2012-2022
Tianjin Medical University
2018-2022
Research Triangle Park Foundation
2021
Bayer (United States)
2021
Hebei Medical University
2016
Botswana Geoscience Institute
2015
Genomics (United Kingdom)
2015
University of Missouri
2005-2011
Bayer (Germany)
2011
Significance Early branching events in the diversification of land plants and closely related algal lineages remain fundamental unresolved questions plant evolutionary biology. Accurate reconstructions these relationships are critical for testing hypotheses character evolution: example, origins embryo, vascular tissue, seeds, flowers. We investigated among streptophyte algae using largest set nuclear genes that has been applied to this problem date. Hypothesized were rigorously tested...
Abstract Background Although it is agreed that a major polyploidy event, gamma, occurred within the eudicots, phylogenetic placement of event remains unclear. Results To determine when this polyploidization relative to speciation events in angiosperm history, we employed phylogenomic approach investigate timing gene set duplications located on syntenic gamma blocks. We populated 769 putative families with large sets homologs obtained from public transcriptomes basal angiosperms, magnoliids,...
Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify quality RNA, there have been no large-scale empirical evaluations major determinants success. We used combination existing newly developed methods isolate total RNA from 1115 samples 695 plant species 324 families, which represents >900 million years phylogenetic diversity green algae through flowering plants, including many plants...
The cultivated [Glycine max (L) Merr.] and wild soja Siebold & Zucc.] soybean species comprise wide variation in seed composition traits. Compared to soybean, contains low protein, high oil, sucrose. In this study, an interspecific population was derived from a cross between G. (Williams 82) (PI 483460B). This recombinant inbred line (RIL) of 188 lines sequenced at 0.3× depth. Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events RILs were defined, high-resolution bin...
Abstract We report characteristics of soybean genetic diversity and structure from the resequencing 481 diverse accessions, comprising 52 wild ( Glycine soja ) selections 429 cultivated max varieties (landraces elites). This data was used to identify 7.8 million SNPs, predict SNP effects relative genic regions, structure, relationships, linkage disequilibrium. found evidence distinct, mostly independent selection lineages by particular geographic location. Among varieties, we identified...
Abstract Background With the advance of new massively parallel genotyping technologies, quantitative trait loci (QTL) fine mapping and map-based cloning become more achievable in identifying genes for important complex traits. Development high-density genetic markers QTL regions specific populations is essential fine-mapping economically genes. Single nucleotide polymorphisms (SNPs) are most abundant form variation existing between any diverse genotypes that usually used studies. The...
Abstract Background Soybean ( Glycine max [L] Merr.) seed isoflavones have long been considered a desirable trait to target in selection programs for their contribution human health and plant defense systems. However, attempts modify isoflavone contents not always produced the expected results because genetic basis is polygenic complex. Undoubtedly, extreme variability that display over environments has obscured our understanding of genetics involved. Results In this study, mapping...
Liver cancer is one of the most common cancers worldwide. CDR3 sequencing-based immune repertoire can be closely associated with prognosis and development. Identifying specific interaction between TCR cellular antigens important for developing novel immunotherapeutic approaches treatment cancer. The rearranged TCRβ loci amplified using Vβ- Jβ-specific primers by multi-PCR sequenced high-throughput sequencing (HTS) in liver were compared those T cells from healthy adult peripheral blood...
Abstract Coinfection, the simultaneous invasion of multiple pathogens into a single host, is critical but understudied area, especially in farm animal sector. We report unique and unusual fatal case coinfection with S . Indiana Kentucky, which has rarely been studied literature could hold potential importance for veterinary clinics. In silico analysis revealed that all isolates exhibited extensive multidrug resistance. By analyzing plasmids, two replicons, IncHI2 IncHI2A, were detected...
Immunoglobulin A Nephropathy (IgAN) is the most common glomerulonephritis worldwide. The pathologic hallmark of IgAN immune complex deposited in glomerular mesangium, which induces inflammation and affects kidney's normal functions. exact pathogenesis IgAN, however, remains obscure. Further, current clinical practice, diagnosis relies on needle biopsy renal tissue. Therefore, a non-invasive method for prognosis surveillance disease highly desirable. To this end, we investigated T cell...
Wild soybean species (Glycine soja Siebold & Zucc.) comprise a unique resource to widen the genetic base of cultivated [Glycine max (L.) Merr.] for various agronomic traits. An inter-specific mapping population derived from cross cultivar Williams 82 and PI 483460B, wild accession, was utilized characterization root architecture The objectives this study were identify characterize quantitative trait loci (QTL) seedling shoot traits, as well determine additive/epistatic interaction effects...
Abstract Primary biliary cholangitis (PBC) is an autoimmune liver disease characterized by elevated serum anti-mitochondrial Ab and lymphocyte-mediated bile duct damage. This study was designed to reveal the clonal characteristics of B lymphocyte repertoire in patients with PBC facilitate better understanding its pathogenesis management these patients. Using high-throughput sequencing Ig genes, we analyzed circulating lymphocytes 43 PBC, 34 age- gender-matched healthy controls. Compared...
Abstract This work focused on the separation of active ingredients maca ( Lepidium meyenii Walpers) and evaluated antioxidative capability these components with effects improving glucose lipid metabolism in insulin‐resistant HepG2 cells. DPPH free radical scavenging reducing power assays were used to evaluate antioxidant activity extracts. An cell model induced by glucose, fructose, oleic acid, palmitic acid was adopted investigate extracts regulating this study. LC‐MS/MS then for...
Abstract Immunoglobulin A Nephropathy (IgAN) is the most common glomerulonephritis worldwide. In IgAN, immune complex deposite in glomerular mesangium, which induce inflammation and affect kidney’s normal functions. However, exact pathogenesis of IgAN still incompletely understood. Further, current practice clinical diagnosis relies on needle biopsy renal tissue. Therefore, a non-invasive method for prognosis surveillance disease high demand. this paper, we investigated both T cell receptor...
Methods for RNA isolation These methods were originally included in Appendix S1 of "Evaluating Isolating Total and Predicting the Success Sequencing Phylogenetically Diverse Plant Transcriptomes" Marc T. J. Johnson et al. PLOS ONE, November 21, 2012. https://doi.org/10.1371/journal.pone.0050226 Many protocols share elements or combine components from several methods. For each method, we describe reagents procedures used, identify researchers institute that implemented protocol. Due to...
This protocol is part of a collection eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6) and was originally published as Appendix S1 "Evaluating Methods for Isolating Total Predicting the Success Sequencing Phylogenetically Diverse Transcriptomes" Marc T. J. Johnson et al. PLOS ONE, November 21, 2012. https://doi.org/10.1371/journal.pone.0050226