High expression of the long non-coding RNA nuclear-enriched abundant transcript 1 (NEAT1) in whole blood has been reported colorectal cancer patients; however, its' clinical significance and origin are unclear. We evaluated diagnostic prognostic value, NEAT1 cancer. Expression variants, NEAT1_v1 NEAT1_v2 were determined using real-time quantitative PCR. The value was test (n = 60) validation 200) cohorts patients normal controls (NCs). To identify NEAT1, its analyzed blood, matched primary...

Long noncoding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules, which were involved in broad range biological processes and complex diseases. Research on lncRNAs may shed light tumorigenesis progression colorectal cancer (CRC). The purpose the present study was to identify correlated with CRC then investigate their potential functions. We selected 92 patients for this prospective collected tumor samples clinical records. First, global lncRNA expression profiles...

Objective To compare the long-term survival of colorectal cancer (CRC) in young patients with elderly ones. Methods Using Surveillance, Epidemiology, and End Results (SEER) population-based data, we identified 69,835 non-metastatic diagnosed between January 1, 1988 December 31, 2003 treated surgery. Patients were divided into (40 years under) groups (over 40 age). Five-year specific data obtained. Kaplan-Meier methods adopted multivariable Cox regression models built for analysis outcomes...

The upregulated expression of CXCL1 has been validated in colorectal cancer patients. As a potential biotherapeutic target for cancer, the mechanism by which affects development is not clear.Expression data were obtained from GEO database and verified using GEPIA TIMER 2.0 database. Knockout overexpression cells CRISPR/Cas "Sleeping Beauty" transposon-mediated gene editing techniques. Cell biological function was demonstrated CCK-8, transwell chamber Colony formation assay. RT-qPCR Western...

The influence of natural regulatory T cells (nTregs) on the patients with colon cancer is unclear. Demethylated status Treg-specific demethylated region (TSDR) FOXP3 gene was reported to be a potential biomarker for identification nTregs. demethylation rate TSDR (TSDR-DMR) calculated by using methylation-specific quantitative polymerase chain reaction (MS-qPCR) assay. expression TSDR-DMR and mRNA investigated in various colorectal cell lines. A total 130 carcinoma samples were utilized study...

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a chemotactic cytokine known to regulate cancer progression and invasion. However, the prognostic significance of CXCL1 expression in colorectal (CRC) has not been fully characterized. The present study explored clinicopathological potential role carcinogenesis CRC. protein was measured immunohistochemically tissue microarrays constructed from 276 CRC patients. levels their associations with characteristics patient survival were evaluated. effect...

450 Background: Tisle is an anti-PD-1 antibody. The combination of + XELOX/FP a first-line treatment AGC in China. Both irinotecan and paclitaxel have also shown antitumor activity AGC. In this phase I/II study, we explore the safety, tolerability, efficacy POFI as HER-2 negative, pMMR Methods: I dose finding study using standard 3+3 design, subjects received four escalating levels (dl) irinotecan/paclitaxel (mg/m 2 ): 135/45 (dl #1), 150/45 #2), 135/67.5 #3), 135/90 #4) with tisle 200 mg...

Background Although immune checkpoint inhibitors (ICIs) and anti-angiogenic drugs have demonstrated effectiveness in treating advanced gastric cancer (GC), their role neoadjuvant or conversion therapy remains uncertain. This study aimed to evaluate the efficacy safety of combining chemotherapy with anti-angiogenesis ICIs patients locally GC (LAGC). Methods In this cohort study, we reviewed our prospectively maintained database included individuals diagnosed clinical stage II-III who received...

Abstract Background Colorectal cancer (CRC) is highly prevalent worldwide, with more patients experiencing colorectal liver metastases (CRLM). This study aimed to identify key genes in CRLM through single-cell sequencing data reanalysis and experimental validation. Methods The analyzed RNA-sequencing (scRNA-seq) from the Gene Expression Omnibus (GEO) database. ontology (GO) Kyoto Encyclopedia of Genes Genomes (KEGG) were used for gene functional enrichment analysis. Cancer Genome Atlas...

// Qingguo Li 1, 2 , Changhua Zhuo 2, 3 Guoxiang Cai Dawei Lei Liang Sanjun 1 Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, People's Republic China Oncology, Medical College, University, Surgical Fujian Provincial Hospital, Teaching Hospital Fuzhou, Correspondence to: Cai, e-mail: caisanjun_sh@163.com Keywords: Rectal cancer, negative lymph nodes, prognosis, SEER Received: June 12, 2014 Accepted: September 29, Published: October 20, ABSTRACT...

Colorectal cancer is one of the most common malignancies worldwide. Liver metastasis major direct cause colorectal cancer-related deaths. Although radical resection effective treatment for liver metastasis, several patients are not eligible surgery. Therefore, there a need to develop novel treatments based on understanding biological mechanisms underlying in cancer. This study demonstrated that activin A/ACVR2A inhibits colon cell migration and invasion, as well suppresses...

Recent studies indicated that preoperative radiotherapy significantly reduces the lymph nodes (LNs) harvest from patients with rectal cancer. This may weaken prognostic value of current standard LNs retrieval (≥12 LNs). study investigates impact LN counts on pathologically LN-negative (ypN0) after for Surveillance, Epidemiology and End Results (SEER) registered nonmetastatic cancer diagnosed between 1998 2005 were included in this study. Optimal cutoff number retrieved was determined by...

To evaluate the safety and feasibility of laparoscopic abdominoperineal resection compared with open procedure in multimodality management rectal cancer.A total 106 cancer patients who underwent (OAPR) were matched (LAPR) a 1 to fashion, between 2009 2013 at Fudan University Shanghai Cancer Center. Propensity score matching was carried out based on age, gender, pathological staging disease administration neoadjuvant chemoradiation. Data regarding preoperative staging, surgical technique,...

CD 4 + 25 regulatory T (Treg) cells comprise a unique subset of required for maintaining immune homeostasis. However, the molecular mechanisms associated with functional variety Treg are not fully delineated. In present study, we demonstrate that ubiquitin‐specific protease ( USP )4 physically interacted interferon factor 8 IRF 8) function via K48‐linked deubiquitinase, which stabilized protein levels in cells. Depletion promoted polyubiquitination and upregulation type 2 inflammatory...

Abstract Chinese guidelines recommend POF (paclitaxel, oxaliplatin, and 5‐FU/levoleucovorin) as first‐line treatment for advanced gastric cancer (AGC). Apatinib can augment the antitumor effect of paclitaxel, or fluorouracil in preclinical studies AGC. A phase I clinical trial was conducted to evaluate anticancer activity maximum tolerated dose (MTD) apatinib plus treatment‐naïve patients with AGC establish a recommended II dose. Participants received escalating doses daily oral (250, 375,...

The natural history and appropriate management of anastomotic sinus has not been clearly defined. aim this study was to evaluate the incidence, outcomes sinus.The medical records all patients who underwent a low anterior resection (LAR) or an ileal pouch-anal anastomosis (IPAA) with diverting loop ileostomy (LI) contrast enema performed before planned stoma closure between 2001 2011 were retrospectively reviewed. radiological features tract, treatment outcome studied.Twenty (8.2%) found have...

Abstract In contrast to colonoscopy biopsy, which contains several disadvantages such as bleeding, sampling error, crush artifact, and time‐consuming pathological procedure, multiphoton microscopy (MPM) enables direct noninvasive visualization of tissue architecture cell morphology in live tissues without the administration exogenous agents. We performed a proof‐of‐principle study evaluate feasibility using MPM make real‐time optical diagnosis colorectal cancer by investigating 30 fresh,...