A5102997000- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Organ Transplantation Techniques and Outcomes
- RNA modifications and cancer
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Asthma and respiratory diseases
- RNA Research and Splicing
- Pediatric health and respiratory diseases
- Aldose Reductase and Taurine
- Circular RNAs in diseases
- Nutrition and Health in Aging
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Cancer Mechanisms and Therapy
- Circadian rhythm and melatonin
- Cancer Immunotherapy and Biomarkers
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Cells and Metastasis
Southwest University
2023-2025
Huazhong University of Science and Technology
2025
Shanghai Jiao Tong University
2017-2024
National Clinical Research
2024
Soochow University
2022-2024
First Affiliated Hospital of Soochow University
2022-2024
Dalian Medical University
2010-2024
Second Affiliated Hospital of Dalian Medical University
2010-2024
Beijing University of Chinese Medicine
2024
Huazhong Agricultural University
2024
Cancer metastasis is determined by the formation of metastatic niche and ability cancer cells to adapt microenvironmental stresses. Anoikis resistance a fundamental feature cell survival during progression. However, mechanisms underlying anoikis in ovarian are still unclear.Expressions miRNA-141 its downstream targets were evaluated qPCR, Western blotting, Immunohistochemical (IHC) situ hybridization (ISH) assays. The luciferase assays used prove KLF12 as target miR-141. cDNA microarray...
Abstract Alpha-enolase (ENO1) has been found to be dysregulated in several human malignancies, including hepatocellular carcinoma (HCC). Although the role of ENO1 as a glycolytic enzyme HCC cells well characterized, little is known about other roles ENO1, especially exosome-derived regulating progression. Here, we demonstrated that frequently upregulated or tissues, with even higher expression highly metastatic tissues exosomes derived from sources. Moreover, associated tumor-node-metastasis...
Objectives N 6 -methyladenosine (m A) RNA methylation is implicated in the progression of multiple cancers via influencing mRNA modification. YTHDF1 can act as an oncogene gastric cancer (GC), while biological mechanisms which regulates tumorigenesis through m A modification remain largely unknown. Methods GEO and TCGA cohorts were analyzed for differentially expressed components GC clinical specimens their association with prognosis. Transwell flow cytometry assays well subcutaneous...
Context: The development of thyroid-associated ophthalmopathy (TAO) is associated with self-immune dysfunction. Recent findings in TAO and Graves' disease indicate that IL-17A may also be involved the autoimmunity TAO. Objective: We sought to investigate pathogenic function IL-17A-producing T cells Design/Setting/Participants: Blood samples orbital fibroblasts (OFs) were collected from patients healthy subjects. Main Outcome Measures: Flow cytometry, real-time PCR, cytokine-specific ELISA,...
Intracerebral hemorrhage (ICH) is a devastating form of stroke with high morbidity and mortality. Heme oxygenase-1 (HO-1), the key enzyme in heme degradation, highly expressed after ICH, but its role still unclear. In this study, we used an HO-1 inducer inhibitor to test different stages ICH vivo vitro. early stage expression worsened outcomes mice subjected collagenase-induced model. increased brain edema, white matter damage, neuronal death, neurobehavioral deficits. Furthermore, elevated...
Abstract FOXP3 + Regulatory T (Treg) cells play a key role in the maintenance of immune homeostasis and tolerance. Disruption Foxp3 expression results generation instable Treg acquisition effector T-cell-like function. Here we report that E3 deubiquitinase USP21 prevents depletion at protein level restricts T-helper-1-like cells. Mice depleted Usp21 specifically display disorders characterized by spontaneous T-cell activation excessive T-helper type 1 (Th1) skewing into Th1-like stabilizes...
Article9 July 2020Open Access Source DataTransparent process The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation Jing Yang Shanghai Institute of Immunology and Department Microbiology, Jiao Tong University School Medicine, University, Shanghai, China Search for more papers this author Ping Wei Otolaryngology, Ministry Education Key Laboratory Child Development Disorders, National Clinical Research Center Health Disorders (Chongqing),...
SUMMARY Background and Purpose: The glial water channel aquaporin‐4 (AQP4) has been shown to be involved in a wide range of brain disorders. Although its important role stroke already documented, the underlying mechanism was not clarified yet. Therefore, this study designed investigate impacts AQP4 deletion ischemia/reperfusion (I/R). Methods Results: Herein we found higher mortality more severe neurological deficits knockout (AQP4 −/− ) mice after transient middle cerebral artery occlusion...
Abstract Objectives FBXW 7 acts as a tumour suppressor by targeting at various oncoproteins for ubiquitin‐mediated degradation. However, the clinical significance and involving regulatory mechanisms of manipulation NSCLC regeneration therapy response are not clear. Materials Methods Immunohistochemical staining qRT ‐ PCR were applied to detect Snai1 expression in 100 samples matched tumour‐adjacent tissues. cancer biological functions studied using MTT assay, immunoblotting, flow cytometry,...
Abstract Cancer-associated fibroblasts (CAF) constitute a major component of the tumor microenvironment. The effects CAFs on progression colorectal cancer remain controversial. In this study, we found ectopic overexpression Fibronectin leucine-rich transmembrane protein 3 (FLRT3) inhibited process epithelial–mesenchymal transition (EMT), as well proliferation, migration, invasion, and promote apoptosis cells, whereas silencing FLRT3 expression resulted in opposite phenomenon. downregulation...
Reactive oxygen species (ROS) are involved in pathophysiology of ischemia/reperfusion injury. Melatonin is a potent scavenger ROS. Thus, this study was designed to elucidate its effects combined hepatic warm ischemia and resection model. The right lateral caudate lobes (32% liver volume) Sprague-Dawley rats underwent for 30 min followed by reperfusion subsequent the nonischemic tissue. Some were gavaged with 50 mg/kg melatonin 2 hr before onset experiments. Controls received same volume...
The CD4+CD25+FOXP3+ regulatory T (Treg) cells are critical for maintaining immune tolerance in healthy individuals and reported to restrict anti-inflammatory responses thereby promote tumor progression, suggesting them as a target the development of antitumor immunotherapy. Forkhead box P3 (FOXP3) is key transcription factor governing Treg lineage differentiation their immune-suppressive function. Here, using cells, well HEK-293T Jurkat we report that stability FOXP3 directly positively...
Abstract Numerous data show that taraxacum officinale extract (TOE) exerts protective effects on inflammatory diseases. However, the underlying mechanisms by which TOE affects dextran sulphate sodium (DSS)‐induced colitis remain unclear. After DSS‐induced were treated with different concentrations of for 8 days, bodyweight, disease activity index (DAI), colon lengths and pathological scoring assessed, histopathological examination was confirmed HE staining. Furthermore, a transcriptome...
In this research, we aimed to resolve contradictory results whether SOX9 plays a positive or negative role in melanoma progression and determine its closely related member SOX10 share the same distinct targets mediating their functions melanoma. Immunofluorescence, TCGA database qPCR were used analyze correlation between expression patterns levels of SOX9, NEDD9 patient samples. AlamarBlue, transwell invasion colony formation assays cell lines conducted investigate epistatic relationship...
The ability of melanoma to acquire metastasis through the induction angiogenesis is one major causes skin cancer death. Here, it found that high transcript levels DEP domain containing 1B (DEPDC1B) in cutaneous melanomas are significantly associated with a poor prognosis. Tissue microarray analysis indicates DEPDC1B expression positively correlated SOX10 different stages melanoma. Consistently, both required and sufficient for growth, metastasis, angiogenesis, functions as direct downstream...
The FK506-binding protein 51 (FKBP51, encoded by FKBP5 gene) has emerged as a critical regulator of mammalian endocrine stress responses and potential pharmacological target for metabolic disorders, including type 2 diabetes (T2D). However, in β cells, which secrete the only glucose-lowering hormone-insulin, expression function not been documented. Here, using human pancreatic tissue primary islets, we demonstrated abundant displayed an responsive induction upon acute inflammatory mimicked...