Abstract Triple‐negative breast cancer ( TNBC ) is the most aggressive subtype with a poor prognosis. The micro RNA ‐200 (miR‐200) family has been associated metastasis. However, epigenetic mechanisms underlying miR‐200b repression in are not fully elucidated. In this study, we found that MYC proto‐oncogene, bHLH transcription factor (MYC) and DNA methyltransferase 3A DNMT 3A) were highly expressed tissues compared other subtypes, while expression was inhibited significantly. We demonstrated...

LCL161, a novel Smac mimetic, is known to have anti-tumor activity and improve chemosensitivity in various cancers. However, the function mechanisms of combination LCL161 paclitaxel non-small cell lung cancer (NSCLC) remain unknown. Cellular inhibitor apoptotic protein 1 2 (cIAP1&2) expression NSCLC tissues adjacent non-tumor were assessed by immunohistochemistry. The correlations between cIAP1&2 clinicopathological characteristics, prognosis analyzed. Cell viability apoptosis measured MTT...

Tamoxifen (TAM) resistance is a substantial challenge in the treatment of estrogen receptor (ER)-positive breast cancer. Previous studies have revealed an important role microRNA (miRNA/miR)-26a TAM However, mechanism underlying regulatory effects miR-26a on remains to be elucidated. The expression levels ER-positive cancer were detected by reverse transcription-quantitative polymerase chain reaction. E2F transcription factor 7 (E2F7) and MYC proto-oncogene, bHLH (MYC) western blotting....

Abstract Objectives FBXW 7 acts as a tumour suppressor by targeting at various oncoproteins for ubiquitin‐mediated degradation. However, the clinical significance and involving regulatory mechanisms of manipulation NSCLC regeneration therapy response are not clear. Materials Methods Immunohistochemical staining qRT ‐ PCR were applied to detect Snai1 expression in 100 samples matched tumour‐adjacent tissues. cancer biological functions studied using MTT assay, immunoblotting, flow cytometry,...

Accumulating evidence has indicated that long noncoding RNAs (lncRNAs) play pivotal roles in the processes of cancer occurrence, progression, and treatment. FAM83A-AS1 is a novel onco-lncRNA involved various cancers. Nevertheless, biological function underlying mechanism lung adenocarcinoma (LUAD) remain largely unclear. In this study, we found to be upregulated LUAD tissues closely associated with tumor size, lymph node metastasis, TNM stage. addition, high expression correlated positively...

The small tyrosine kinase inhibitors (TKIs) subversively altered the lung cancer treatments, but patients will inevitably face therapy resistance and disease recurrence. We aim to explore potential roles of non-coding RNAs in sensitizing TKIs effects. Multiple cellular molecular detections were applied confirm mechanistic regulations intracellular connections. explored specific gene features candidates association with resistance, found that m6A controlled stemness EMT through METTL3 YTHDF2....

Abstract Background Cancer stem cells (CSCs) are a small population of in tumor tissues that can drive initiation and promote progression. A number previous studies indirectly mentioned the role F-box WD repeat domain-containing 7 (FBXW7) as suppressor Triple-negative breast cancer (TNBC). However, few have focused on function FBXW7 stemness TNBC related mechanism. Methods We detected by immunohistochemistry (IHC) 80 patients. knockdown overexpression MD-MBA-231 HCC1937 cell models were...

Abstract The tumor microenvironment (TME) plays a pivotal role in the onset, progression, and treatment response of cancer. Among various components TME, cancer-associated fibroblasts (CAFs) are key regulators both immune non-immune cellular functions. Leveraging single-cell RNA sequencing (scRNA) data, we have uncovered previously hidden promising roles within this specific CAF subgroup, paving way for its clinical application. However, several critical questions persist, primarily stemming...

Cancer progression is orchestrated by the accrual of mutations in driver genes, which endow malignant cells with a selective proliferative advantage. Identifying cancer genes crucial for elucidating molecular mechanisms cancer, advancing targeted therapies, and uncovering novel biomarkers. Based on integrated analysis Multi-Omics data Network models, we present MONet, gene identification algorithm. Our method utilizes two graph neural network algorithms protein-protein interaction (PPI)...

Abstract Objectives Breast cancer stem‐like cells (BrCSCs) are the major reason for tumour generation, resistance and recurrence. The turbulence of their self‐renewal ability could help to constrain stem cell expansion. way BrCSCs divided was related capacity, symmetric division contributed a higher ability. Non‐coding long RNA H19 involved in multiple malignant procedures; role mechanistic proof non‐coding controlling divisions were barely known. Materials Methods Indicative functions...

This study was designed to evaluate the prognostic value of preoperative albumin-globulin score (AGS) in patients with non-small cell lung cancer (NSCLC) after pulmonary lobectomy.The optimal cutoff level 40.00 and 27.05 g/L for Alb Glb, respectively. Based on this previous study, both an hypoalbuminemia (< g/L) elevated Glb (≥ were assigned a 2, one or neither 1 0, We investigated correlations between AGS clinicopathological characteristics found that significantly associated TNM stage (P =...

Let-7 microRNAs have been reported to tumor suppressive functions; however, the effect of when used in combination with chemotherapies is uncertain, but may potential for use clinical practice. In this study, we RT-qPCR, western blot analysis, cell proliferation assay, flow cytometry immunohistochemistry (IHC) staining, luciferase assays, sorting analysis and xenografted model explore role chemotherapy sensitivity breast cancer stem cells. The findings current study indicated that Let‑7...

MiR-146a could stimulate tumor growth or block proliferation in systemic malignancies, referring to the specific downstream targeted gene. However, its roles breast cancer stem-like cells (BrCSCs) are barely known. To dig out mechanistic functions, we explored indicative of miR-146 preclinical study, regardless hormone receptor status, and positive correlation between better prognosis was proved, as Let-7c was. uncover implicated mechanisms, first identified suppressive role miR-146a stem...

X-linked inhibitor of apoptosis protein (XIAP) and second mitochondrial-derived activator caspase (Smac) are two important prognostic biomarkers for cancer. They negatively correlated in many types However, their relationship is still unknown lung In the present study, we found that there was a negative correlation between Smac XIAP at level but not mRNA NSCLC patients. overexpression had no effect on degrading endogenous cancer cell lines. Therefore, constructed plasmids with full length...

Snakes are sometimes regarded as pets and used in traditional Chinese medicine. Cryptosporidium spp. frequently identified snakes, representing an important pathogen causing gastrointestinal diseases. Current data indicate that risk factors for infection patterns of clinical symptom presentation may differ among To better understand the status by spp., fecal samples were collected from 603 asymptomatic 147 symptomatic snakes 26 provinces China. These came Elaphe guttata , obsoleta, Pituophis...

// Guodong Xiao 1, * , Xiang Li Gang Boxiang Zhang 1 Chongwen Xu 2 Sida Qin Ning Du Jichang Wang 3 Shou-Ching Tang 4, 5 Jing Hong Ren Ke Chen 6 and Xin Sun Department of Thoracic Surgery Oncology, The Second Surgery, Cancer Center, First Affiliated Hospital Xi'an Jiaotong University, 710061, China Otorhinolaryngology, Vascular Endovascular 4 Breast Program Interdisciplinary Translational Research Team, Georgia Regents University Augusta, 30912, United States Tianjin Medical Institute...

Lung carcinoma tops the categories of cancer related motility, and has been treated as main threat to human health. The functions mechanism FBXW7 controlled lung stem cells' signatures is barely unknown, miR-367 regulations via Wnt signaling have not explored. Cancer cells either ALDH1+ or CD133+ phenotype were found be referred advanced stages in patients with NSCLC (non-small cell carcinoma). To study roles miR-367, we greater level was reserved than that paired adjacent normal tissues,...

Fibronectin is involved in orchestrating many diverse cellular behaviors, including adhesion, invasion, differentiation, and proliferation recently has also been shown to participate the development of chemoresistance. In this study, we found that fibronectin expression was inversely correlated with clinical responses docetaxel treatment non-small cell lung cancer patients. Subsequently, showed pretreatment could enhance viability reduce apoptosis docetaxel-treated cells because induced...

&lt;b&gt;&lt;i&gt;Objectives:&lt;/i&gt;&lt;/b&gt; Oxidized low-density lipoprotein (ox-LDL) may induce autophagy, apoptosis, necrosis or proliferation of cultured endothelial cells depending on the concentration and exposure time. Our previous studies found that ox-LDL for 6 h increases autophagic level human umbilical vein (HUVECs) in a concentration-dependent manner. The present study investigates relationship between autophagy apoptosis HUVECs exposed to ox-LDL....

Antibodies against the immune checkpoint proteins PD-1 and PD-L1 are novel therapeutic drugs for treatment of advanced non-small cell lung cancer (NSCLC). Many clinical trials involving these achieved breakthroughs in patients previously treated NSCLC. However, results studies not consistent. In this report, we performed a meta-analysis to assess efficacy safety anti-PD-1/PD-L1 antibodies compared with docetaxel NSCLC from 5 randomized trials. We demonstrated that antibody therapy groups had...