A5064732491- Osteoarthritis Treatment and Mechanisms
- Total Knee Arthroplasty Outcomes
- Knee injuries and reconstruction techniques
- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Blood transfusion and management
- Advanced materials and composites
- Venous Thromboembolism Diagnosis and Management
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Cancer-related molecular mechanisms research
- Shoulder Injury and Treatment
- 3D Printing in Biomedical Research
- Tendon Structure and Treatment
- Anesthesia and Pain Management
- Sports injuries and prevention
- Bone and Joint Diseases
- Adipose Tissue and Metabolism
- Lower Extremity Biomechanics and Pathologies
- Inflammasome and immune disorders
- Inflammatory mediators and NSAID effects
- Mesenchymal stem cell research
- Hip and Femur Fractures
- Circular RNAs in diseases
- Peroxisome Proliferator-Activated Receptors
Shanghai Changzheng Hospital
2009-2025
Second Military Medical University
2009-2024
Due to a blood supply shortage, articular cartilage has limited capacity for self-healing once damaged. Articular chondrocytes, progenitor cells, embryonic stem and mesenchymal cells are candidate regeneration. Significant current attention is paid improving chondrogenic differentiation capacity; unfortunately, the potential hypertrophy of differentiated largely overlooked. Consequently, engineered tissue actually transient rather than permanent one. The development hypertrophic ends with...
Cyclic strain-induced chondrocyte damage is actively involved in the pathogenesis of osteoarthritis and arthritis. MicroRNAs (miRNAs) carried by exosomes have been implicated various diseases. However, role miR-100-5p cyclic remains to be elucidated. NADPH oxidase 4 (NOX4) were silenced or overexpressed human primary articular chondrocytes. PKH-67 Dye was used trace exosome endocytosis. Reactive oxygen species (ROS) production monitored using DCFH-DA. Cell apoptosis measured a flow...
Chondrocytes are the exclusive cellular constituents of articular cartilage, and their functional status governs health cartilage. The primary factor contributing to deterioration cartilage structure function is chondrocyte senescence. In hypoxia hypodextrose environment, chondrocytes heavily rely on glycolysis for energy metabolism. Mitochondria, acting as regulatory hub metabolism, exhibit dysfunction before senescence, indicating crucial involvement in process. Previous research has...
Here we aimed to determine the therapeutic effect of allicin on ankylosing spondylitis (AS) and explore mechanism(s) action. AS mouse model was constructed by transferring HLA-B2704 gene into Kunming mice verified RT-PCR CT imaging. Verified were randomly divided group (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>6</mml:mn></mml:mrow></mml:math>) allicin-treated groups (50, 100, 200 mg/kg, resp.,<mml:math...
Abstract Background Knee osteoarthritis (KOA) seriously affects the quality of life KOA patients. This study aimed to investigate whether miR-107 could regulate through pyroptosis affect collagen protein secreted by chondrocytes IL-1β. Methods The proliferation was detected CCK-8 assay. RT-qPCR analysis used identify expression and transfection effects. Col II, IL-1β, IL-18, MMP13 in supernatant or ELISA assay western blot analysis. analyzed TUNEL pyroptosis-related proteins blot. Luciferase...
Patellar dysplasia (PD) can cause patellar dislocation and subsequent osteoarthritis (OA) development. Herein, a novel ABCA6 mutation contributing to four-generation family with familiar (FPD) is identified. In this study, whole exome sequencing (WES) genetic linkage analysis across lineage presenting six cases of FPD are conducted. A disease-causing in identified for FPD. Further analyses reveal consistent correlation between expression downregulation PD occurrence, chondrocyte...
MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate of synovium-derived MSCs (SDSCs) and maturation RCJ3.1C5.1 chondrocytes. SDSCs were sourced from knee joint synovium New Zealand white rabbits, their multilineage potentials examined. The level was measured during SDSC differentiation, together with determination markers chondrocytes expression after transfection mimics/inhibitor....
It has rarely been reported about the changes of hemoglobin (Hb) and hematocrit (Hct) in elderly patients receiving total knee arthroplasty (TKA) or hip (THA). This study aimed to evaluate Hb Hct after TKA THA patients, analyze its relationship with sex type arthroplasty.This is a prospective cohort study, including 107 without allogeneic blood transfusion. There were 54 males 53 females, mean age 69.42 years. Levels examined preoperatively during 6 months follow-up operation.Levels...
Abstract Platelet-rich plasma (PRP) that has various growth factors been used clinically in cartilage repair. However, the short residence time and release at injury site limit its therapeutic effect. The present study fabricated a granular hydrogel was assembled from gelatin microspheres tannic acid through their abundant hydrogen bonding. Gelatin with concentration of 10 wt% diameter distribution 1–10 μm were to assemble by form hydrogel, which exhibited elasticity under low shear strain,...
Osteoarthritis (OA) affects approximately 12% of the aging Western population. The sirtuin/forkhead box O (SIRT/FOXO) signaling pathway plays essential roles in various biological processes. Despite it has been demonstrated that ubiquitin-specific protease 3 (USP3) inhibits chondrocyte apoptosis induced by interleukin (IL)-1β, role USP3/SIRT3/FOXO3 senescence chondrocytes OA is unclear. This study initially isolated articular and investigated USP3 IL-1β-induced chondrocytes. After was...
The communication between macrophages and tendon cells plays a critical role in regulating the tendon-healing process. However, potential mechanisms through which can control peritendinous fibrosis are unknown. Our data showed strong pro-inflammatory phenotype of after mouse tendon-bone injury. Moreover, by using small-molecule compound library, we identified an aldehyde dehydrogenase inhibitor, disulfiram (DSF), significantly promote transition macrophage from M1 to M2 decrease phenotype....
To evaluate the effect of drainage tube on prognosis after total knee arthroplasty (TKA) and explore an effective treatment with favorable prognosis.In a prospective study, 18 patients TKA for first time were included randomly divided into three groups, group A (no placement tube), B (negative pressure drainage), C (4 h clamping drainage). Intraoperative postoperative blood loss, operation time, volume recorded analyzed. Arthrocele, ecchymosis, range motion (ROM) examined postoperatively....
Abstract Decellularized extracellular matrix (dECM) retains the bioactive components to mimic tissue‐specific microenvironment. Designing dECM‐based inks for 3D printing prepare scaffolds can enhance regeneration of corresponding tissues. However, due strict requirements inks, how efficiently is a challenge. Inspired by granular lubrication, present study proposes an effective and general method preparing ink from dECM microparticles (MP) with high content. Through dopamine polymerization,...
Background: Articular cartilage is a complex structure that allows for low frictional gliding and effective shock absorption. Various sports injuries inflammatory conditions can lead to lesions in the articular cartilage, which has limited regenerative potential. Type I collagen combined with autologous chondrocytes three-dimensional culture were used induce regeneration of single-layer expanded without chondrogenic differentiation. Purpose: To assess clinical, radiological, histological...
The chondrogenic differentiation of synovial mesenchymal stem cells (SMSCs) is regulated by essential transcription factors and signaling cascades. However, the precise mechanisms involved in this process remain unclear. MicroRNAs (miRs/miRNAs) are undersized non‑coding RNAs responsible for post‑transcriptional regulation gene expression, binding to 3'‑untranslated regions (3'‑UTRs) their target mRNAs. miRNAs may constitute a promising tool regulate SMSC advance controlled SMSCs therapeutic...