A5085790081- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- Water Quality Monitoring and Analysis
- Blind Source Separation Techniques
- Machine Learning in Bioinformatics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Lung Cancer Treatments and Mutations
- Traditional Chinese Medicine Studies
- Chemical Synthesis and Analysis
- Water Treatment and Disinfection
- Bladder and Urothelial Cancer Treatments
- Environmental and Agricultural Sciences
- Analytical Chemistry and Chromatography
- Carbohydrate Chemistry and Synthesis
- Amino Acid Enzymes and Metabolism
- Biological Activity of Diterpenoids and Biflavonoids
- Analytical Methods in Pharmaceuticals
- Protein Kinase Regulation and GTPase Signaling
- Gout, Hyperuricemia, Uric Acid
- Security in Wireless Sensor Networks
- Computational Drug Discovery Methods
- Biocrusts and Microbial Ecology
Novel (United States)
2024
Northwest A&F University
2014-2024
Beijing Institute of Technology
2024
Beijing Information Science & Technology University
2023-2024
Xiamen University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2023
Beijing Proteome Research Center
2019-2023
Beijing YouAn Hospital
2022-2023
Capital Medical University
2022-2023
Hefei University of Technology
2023
Higher-order topological insulators (HOTIs) belong to a new class of materials with unusual phases. They have garnered considerable attention due their capabilities in confining energy at the hinges and corners, which is entirely protected by topology, thus become attractive structures for acoustic wave studies control. However, most practical applications audible low frequencies, compact subwavelength implementations are desirable addition providing robust guiding sound beyond...
Abstract Biomarkers are measurable changes associated with the disease. Urine can reflect of body while blood is under control homeostatic mechanisms; thus, urine considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile urinary proteome will provide a basic understanding proteins. In this paper, we present in-depth analysis based on different separation strategies, including direct one dimensional liquid chromatography–tandem mass...
Abstract Currently, imaging, fecal immunochemical tests (FITs) and serum carcinoembryonic antigen (CEA) are not adequate for the early detection evaluation of metastasis recurrence in colorectal cancer (CRC). To comprehensively identify validate more accurate noninvasive biomarkers urine, we implement a staged discovery-verification-validation pipeline 657 urine 993 tissue samples from healthy controls CRC patients with distinct metastatic risk. The generated diagnostic signature combined...
Urine is an important source of biomarkers. A single proteomics assay can identify hundreds differentially expressed proteins between disease and control samples; however, the ability to select biomarker candidates with most promise for further validation study remains difficult. bioinformatics tool that allows accurate convenient comparison all existing related studies markedly aid development this area. In study, we constructed Urinary Protein Biomarker (UPB) database collect urinary...
Potassium permanganate (KMnO4) preoxidation is capable of enhancing cyanobacteria cell removal. However, the impacts KMnO4 on viability and potential toxin release have not been comprehensively characterized. In this study, Microcystis aeruginosa inactivation degradation intracellular microcystin-LR (MC-LR) other featured organic matter were investigated. oxidation M. exhibited some kinetic patterns that different from standard chemical reactions. Results indicated loss MC-LR both followed...
Disease biomarkers are the measurable changes associated with a pathophysiological process. Without homeostatic control, urine accumulates systematic in body. Thus, is an attractive biological material for discovery of disease biomarkers. One major bottlenecks urinary biomarker that concentration and composition proteins influenced by many physiological factors. To elucidate individual variation related factors influencing proteome, we comprehensively analyzed samples from healthy adult...
Urine and cerebrospinal fluid (CSF) are two important biofluids used for disease biomarker discovery. For differential proteomic analysis, it is essential to evaluate individual gender variations. In this study, we characterized urinary CSF proteomes of 14 healthy volunteers with regard variations using 2DLC-MS/MS analysis 8-plex iTRAQ quantification. A total 968/512 urinary/CSF proteins were identified, 406/280 quantified in all individuals. The median inter-individual coefficients...
Purpose Body fluid is considered a rich source of disease biomarkers. Proteins in many body fluids have potential clinical applications for diagnostic and prognostic predictions. Experimental design To determine differences the protein components functional features fluids, proteomic comparison five (plasma, urine, cerebrospinal fluid, saliva, amniotic fluid) was conducted by high‐resolution mass spectrometry. Results A total 4717 nonredundant proteins were identified, concentrations 3433...
Background Ischemic stroke (IS) is a major global cause of death and disability, linked to nucleotide metabolism imbalances. This study aimed identify metabolism-related genes associated with IS explore their roles in disease mechanisms for new diagnostic therapeutic strategies. Methods gene expression data were sourced from the GEO database. Differential analysis weighted co-expression network (WGCNA) conducted R, intersecting results genes. Functional enrichment connectivity map (cMAP)...
With the help of proteomics technology, human plasma and urine proteomes, which closely represent protein compositions input output kidney, respectively, have been profiled in much greater detail by different research teams. Many datasets accumulated to form "reference profiles" proteomes. Comparing these two proteomes may us understand handling aspect kidney function a way, however, has unavailable until recent advances technology.After removing secreted proteins downstream 2611 1522 were...
Abstract Urine has the potential to become a better source of biomarkers. Urinary proteins are affected by many factors; therefore, differentiating between variables associated with any particular pathophysiological condition in clinical samples is challenging. To circumvent these problems, simpler systems, such as animal models, should be used establish direct relationship disease progression and urine changes. In this study, unilateral ureteral obstruction (UUO) model was observe tubular...
In contrast to blood, which has mechanisms maintain a homeostatic internal environment, urine is more likely reflect changes in the body. As accumulates all types of changes, identifying precise cause proteome challenging and crucial biomarker discovery. To reduce effects both genetic environmental factors on urinary proteome, this study used rat model adriamycin-induced nephropathy resembling human focal segmental glomerulosclerosis (FSGS) development.Urine samples were collected at before...
Diabetic nephropathy (DN) is the leading cause of chronic kidney failure and end-stage disease. More accurate non-invasive test for diagnosis monitoring progression DN urgently needed better care such patients. In this study we utilized urinary glycoproteome to discover differential proteins during course type 2 DN. The glycoproteins from normal controls, normalbuminuira, microalbuminura, macroalbuminuria patients were enriched by concanavalin A (ConA) analyzed 2DLC/MS/MS isobaric tags...
Probiotic treatment alters the abundance of neuropeptides in brain.
Ubiquitin ligases (E3s) confer specificity to ubiquitination by recognizing target substrates. However, the substrates of most E3s have not been extensively discovered, and new methods are needed efficiently comprehensively identify these Mostly, specifically recognize via their protein interaction domains. We developed a novel integrated strategy containing domains on proteomic scale. The binding properties were characterized screening random peptide library using yeast two-hybrid system....
The urinary proteome has been widely used for biomarker discovery. A database from normal humans can provide a background discovery proteomics and candidate proteins/peptides targeted proteomics. Therefore, it is necessary to define the minimum number of individuals required sampling represent proteome. In this study, inter-individual inter-gender variations were taken into consideration achieve representative database. An individual analysis was performed on overnight urine samples 20...
As one of the most important modes transportation, buses play an essential part in people's journeys. However, less has been known about whether and under what circumstances people will choose to travel by bus during COVID-19 epidemic. Based on theory planned behaviour (TPB), study examined how impact different stages (middle stage later stage), quality public transport services fear (FCV-19) are associated with individual choices. An online survey 404 participants from Anhui Province, China...