A5029836219- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Bacterial biofilms and quorum sensing
- Bacterial Infections and Vaccines
- Helicobacter pylori-related gastroenterology studies
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Orthopedic Infections and Treatments
- Inhalation and Respiratory Drug Delivery
- Surgical site infection prevention
- Immunotherapy and Immune Responses
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial agents and applications
- Advanced biosensing and bioanalysis techniques
- Wound Healing and Treatments
- NF-κB Signaling Pathways
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
Army Medical University
2010-2025
Beijing Union University
2024
Army Medical College
2023
Jiangsu Provincial Center for Disease Control and Prevention
2010
Helicobacter pylori evade immune responses and achieve persistent colonization in the stomach. However, mechanism by which H. infections persist is not clear. In this study, we showed that MIR30B upregulated during infection of an AGS cell line human gastric tissues. Upregulation benefited bacterial replication compromising process autophagy infection. As a potential mechanistic explanation for observation, demonstrate directly targets ATG12 BECN1, are important proteins involved autophagy....
Shiga toxins (Stxs) are a family of cytotoxic proteins that lead to the development bloody diarrhea, hemolytic-uremic syndrome, and central nervous system complications caused by bacteria such as S. dysenteriae, E. coli O157:H7 O104:H4. Increasing evidence indicates macroautophagy (autophagy) is key factor in cell death induced Stxs. However, associated mechanisms not yet clear. This study showed Stx2 induces autophagic Caco-2 cells, cultured line model human enterocytes. Inhibition...
Controlled self-assembly has attracted extensive interest in biological and nanotechnological applications. Enzymatic or biocatalytic triggered is widely used for the diagnostic prognostic marker different pathologies because of their nanostructures effects. However, it remains a great challenge to control peptides living cells with high degree spatial temporal precision. Here we demonstrate light-triggered platform that enables spatiotemporal from nanoparticles into nanofibers through...
S. aureus can invade and persist within host cells, including immune which allows it to evade detection clearance. This intracellular persistence contributes chronic recurrent infections, complicating treatment prolonging the disease. Consequently, there is a critical need for an infection model better understand, prevent, treat infections caused by aureus. study indicated that antibiotics effectively eliminated extracellular bacteria but could not eradicate those had entered cells. Thus,...
Drug efflux systems have recently been recognized as a significant mechanism responsible for multidrug resistance in bacteria. In this study, we described the identification and characterization of new chromosomally encoded pump (SA00565)
Abstract Mitophagy is critical for mitochondrial quality control and function to clear damaged mitochondria. Here, we found that Burkholderia pseudomallei maneuvered host mitophagy its intracellular survival through the type III secretion system needle tip protein BipD. We identified BipD, interacting with BTB-containing proteins KLHL9 KLHL13 by binding Back Kelch domains, recruited NEDD8 family RING E3 ligase CUL3 in response B. infection. Although evidently not involved regulation of...
Abstract Many pathogens secrete effectors to hijack intracellular signaling regulators in host immune cells promote pathogenesis. However, the pathogenesis of Staphylococcus aureus secretory within is unclear. Here, we report that secretes extracellular fibrinogen-binding protein (Efb) into cytoplasm macrophages suppress immunity. Mechanistically, RING finger 114, a E3 ligase, mediates K27-linked ubiquitination Efb at lysine 71, which facilitates recruitment tumor necrosis factor receptor...
Drug efflux systems have recently been recognized as an important mechanism of multidrug resistance in bacteria. Here, we described the identification and characterization a novel chromosomally encoded pump (SA09310) Staphylococcus aureus. SA09310 is 43-kDa protein with 12 transmembrane helices. The conserved amino acid sequence motifs major facilitator superfamily (MFS) were identified SA09310, which indicated that belonged to MFS transporters. Expression sa09310 gene was induced by...
Treating orthopedic implant-associated infections, especially those caused by Staphylococcus aureus (S. aureus), remains a significant challenge. S. has the ability to invade host cells, enabling it evade both antibiotics and immune responses during infection, which may result in clinical treatment failures. Therefore, is critical identify cell type of intracellular infections develop strategy for highly targeted delivery cells. Introduced an antibody-antibiotic conjugate (AAC) elimination...
The development of new antibiotics continues to pose challenges, particularly considering the growing threat multidrug-resistant Staphylococcus aureus. Structurally diverse natural products provide a promising source antibiotics. Herein, we outline concise approach for collective asymmetric total synthesis polycyclic xanthene myrtucommulone D and five related congeners. strategy involves rapid assembly challenging benzopyrano[2,3-a]xanthene core, highly diastereoselective establishment three...
Abstract The increasing prevalence of antibiotic resistance by Pseudomonas aeruginosa (PA) raises an urgent need for effective vaccine. outer membrane proteins PA, especially those that are upregulated during infection, ideal vaccine targets. However, the strong hydrophobicity these hinders their application this purpose. In study, we selected eight from PA with most significantly expression. Their extracellular loops were analyzed and screened using sera patients who had recovered...
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant and fatal pathogens at wound infection sites. MRSA difficult to treat because its antibiotic resistance ability form biofilms site.In this study, a novel nanoscale liquid film-forming system (LFFS) loaded with benzalkonium bromide was produced based on polyvinyl alcohol chitosan.This LFFS showed faster more potent effect against MRSA252 than aqueous solution both in vitro vivo. Additionally, had stronger...
Innate immune effectors constitute the first line of host defense against pathogens. However, roles these are not clearly defined during Klebsiella pneumoniae (K. pneumoniae) respiratory infection. In current study, we established an acute pneumonia model K. infection in mice and confirmed that injury was most severe 48 h post Flow cytometric assay demonstrated alveolar macrophages were predominant cells BALF before infection, neutrophils quickly recruited after this consistent with kinetics...
As TLR2 agonists, several lipopeptides had been proved to be candidate vaccine adjuvants. In our previous study, mimicking N-terminal structures of the bacterial lipoproteins were also able promote antigen-specific immune response. However, structure-activity relationship as agonists is still unclear. Here, 23 synthetic with same lipid moiety but different peptide sequences synthesized, and their activities in vitro mucosal adjuvant effects OVA evaluated. LP1-14, LP1-30, LP1-34 LP2-2...
The latest study shows that gastric cancer (GC) ranked the fifth most common (5.6%) with over 1 million estimated new cases annually and fourth cause of death (7.7%) globally in 2020. Metastasis is leading GC treatment failure. Therefore, clarifying regulatory mechanisms for metastatic process necessary. In current study, we discovered calreticulin (CALR) was highly expressed tissues related to lymph node metastasis patient's terrible prognosis. introduction CALR dramatically promoted cell...
Staphylococcus aureus is a leading pathogen that currently the most common cause of infection in hospitalized patients. An in-depth genetic analysis S. virulence genes contributing to pathogenesis needed develop novel antimicrobial therapies. However, tools for manipulation are limited, particularly those gene expression. Here, 38 highly expressed were identified USA300_FPR3757 via RNA-seq. Promoter regions from 30 these successfully cloned, which 20 promoters exhibited wide range activity....
The use of antibiotic adjuvants is a complementary strategy to the development new antibiotics. essential role ArnA dehydrogenase domain (ArnA_DH) in addition 4-amino-L-arabinose (L-Ara4N) lipid A makes it potential target polymyxin adjuvant design.This study aimed identify inhibitor that enhances antibacterial effect B (PB) and further understand mechanism this drug combination.A susceptible K. pneumoniae strain, ATCC13883, was used screen library based on...
The emergence of polymyxin B (PB) resistant Gram-negative bacteria poses an important clinical and public health threat. Antibiotic adjuvants development is a complementary strategy that fills the gap in new antibiotics. Here, we described discovery enhancement capacity compound 666-15, previously identified as inhibitor cyclic adenosine monophosphate response element-binding protein (CREB), on activity PB against Klebsiella pneumoniae vitro vivo. Mechanistic studies showed this reduced...