A5090534392- Spinal Cord Injury Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Glioma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Spinal Dysraphism and Malformations
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cervical and Thoracic Myelopathy
- Aortic Disease and Treatment Approaches
- Spinal Fractures and Fixation Techniques
- Cardiac Arrest and Resuscitation
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Muscle activation and electromyography studies
- Medical Imaging and Analysis
- Bacterial biofilms and quorum sensing
- Ultrasound and Hyperthermia Applications
- Neuroscience and Neural Engineering
- Cancer Research and Treatments
- Advanced Sensor and Energy Harvesting Materials
- Ultrasound Imaging and Elastography
- Spine and Intervertebral Disc Pathology
- Immune cells in cancer
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2017-2024
University of Baltimore
2020
Johns Hopkins Hospital
2020
Oxford University Press (United Kingdom)
2020
Background Most electronic-cigarette liquids contain propylene glycol, glycerin, nicotine and a wide variety of flavors which many are sweet. Sweet classified as saccharides, esters, acids or aldehydes. This study investigates changes in cariogenic potential when tooth surfaces exposed to e-cigarette aerosols generated from well-characterized reference e-liquids with sweet flavors. Methods Reference were prepared by combining 20/80 glycol/glycerin (by volume fraction), 10 mg/mL nicotine,...
Abstract Translation of biomaterial‐based nanoparticle formulations to the clinic faces significant challenges including efficacy, safety, consistency and scale‐up manufacturing, stability during long‐term storage. Continuous microfluidic fabrication polymeric nanoparticles has potential alleviate associated with manufacture, while offering a scalable solution for clinical level production. Poly(beta‐amino esters) (PBAE)s are class biodegradable cationic polymers that self‐assemble anionic...
Robot-assisted pedicle screw placement is associated with greater accuracy, reduced radiation, less blood loss, shorter hospital stays, and fewer complications than freehand placement. However, it can be longer operative times an extended training period. We report the initial experience of a surgeon using robot system at academic medical center.We retrospectively reviewed all patients undergoing robot-assisted single tertiary care institution by 1 from 10/2017 to 05/2022. Linear regression,...
Replicating spinal cord injury (SCI) in large animals is necessary for evaluating therapeutics potential human translation, yet there currently no commercial, standardized device inducing SCI. We present the fabrication and testing of a custom impactor producing repeatable contusion SCI porcine models. first designed built device. Mechanical modeling was subsequently utilized to calibrate our benchtop setup. Benchtop verification performed measure impact force post calibration. then used...
INTRODUCTION: Following spinal cord injury (SCI), treatment includes vasopressors to elevate mean arterial pressure (MAP) preserve blood flow at the injury. However, this is based on limited understanding of how interact with vasculature. Specifically, α- and β-adrenergic receptors have been implicated in vasoconstriction vasodilation, respectively, central nervous system. Depending affinity pressors each receptor, differential effects may occur. METHODS: A T4-T6 laminectomy was performed...
INTRODUCTION: There is conflicting literature regarding the pathophysiology of spontaneous neurologic deficits following posterior cervicothoracic decompression for chronic spinal cord compression and myeloradiculopathy. While perfusion changes occurring intraoperatively a prevailing theory, there paucity in imaging modalities to assess quantify perfusion. Contrast enhanced ultrasound (CEUS) shows great promise evaluating neural structures. We hypothesized that before after chronically...
Abstract Background The tumor-selective human adenovirus Delta24-RGD is currently under investigation in phase II clinical trials for patients with recurrent glioblastoma (GBM). To improve treatments GBM, we explored the potential of combining antibodies targeting immune checkpoints. Methods C57BL/6 mice were intracranially injected GL261 cells and treated a low dose virus. expression dynamics 10 co-signaling molecules known to affect activity was assessed tumor-infiltrating by flow...
The mechanism by which cationic polymers containing titratable amines mediate effective endosomal escape and cytosolic delivery of nucleic acids is not well understood despite the decades research devoted to these materials. Here, we utilize multiple assays investigating step associated with plasmid polyethylenimine (PEI) poly(β-amino esters) (PBAEs) improve understanding how enable gene delivery. To probe role materials in facilitating escape, utilized vesicle membrane leakage extracellular...
Clinical trials involving anti-programmed cell death protein-1 (anti-PD-1) failed to demonstrate improved overall survival in glioblastoma (GBM) patients. This may be due the expression of alternative checkpoints such as B- and T- lymphocyte attenuator (BTLA) on several immune types including regulatory T cells. Murine GBM models indicate that there is significant upregulation BTLA tumor microenvironment (TME) with associated exhaustion. We investigate use antibodies against PD-1 reversing...
Development of highly effective nonviral gene delivery vectors for transfection diverse cell populations remains a challenge despite utilization both rational and combinatorial driven approaches to nanoparticle engineering. In this work, multifunctional polyesters are synthesized with well-defined branching structures via A2 + B2/B3 C1 Michael addition reactions from small molecule acrylate amine monomers then end-capped amine-containing molecules assess the influence polymer structure on...
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Abstract Background Tension in the spinal cord is a trademark of tethered syndrome. Unfortunately, existing tests cannot quantify tension across bulk cord, making diagnostic evaluation stretch ambiguous. A potential non-destructive metric for ultrasound-derived shear wave velocity (SWV). The sensitive to tissue elasticity and boundary conditions including strain. We use term Ultrasound Tensography describe acoustic with SWV. Methods Our solution Tethered Assessment (TAUT) was utilized three...
There is a need for new tools to better quantify intracellular delivery barriers in high-throughput and high-content ways. Here, we synthesized triple-fluorophore-labeled nucleic acid pH nanosensor measuring of exogenous DNA at specific time points manner by flow cytometry following non-viral transfection. By including two pH-sensitive fluorophores one pH-insensitive fluorophore the nanosensor, detection was possible over full physiological range. We further assessed correlation between...
Abstract BACKGROUND Cerebral vasospasm is a major source of morbidity and mortality following aneurysm rupture has limited treatment options. OBJECTIVE To evaluate the role programmed death-1 (PD-1) in cerebral vasospasm. METHODS Endovascular internal carotid artery perforation (ICAp) was used to induce mice. therapeutic potential targeting PD-1, death ligand-1 (PD-L1) administered 1 h after ICAp measured histologically at level ICA bifurcation bilaterally. PD-1 expressing immune cell...
Immune checkpoint inhibitors such as anti-programmed cell death protein 1 (anti-PD-1) have shown promise for the treatment of cancers melanoma, but results glioblastoma (GBM) been disappointing thus far. It has suggested that GBM multiple mechanisms immunosuppression, indicating a need combinatorial strategies. is well understood increases glutamate in tumor microenvironment (TME); however, significance this not understood. The authors posit upregulation TME immunosuppressive. utilized novel...
Cardiac arrest (CA) remains the leading cause of coma, and early arousal recovery indicators are needed to allocate critical care resources properly. High-frequency oscillations (HFOs) somatosensory evoked potentials (SSEPs) have been shown indicate responsive wakefulness days following CA. Nonetheless, their potential in acute phase, where injury is reversible, has not tested. We hypothesize that time-frequency (TF) analysis HFOs can determine phase. To test our hypothesis, eleven adult...
Low-intensity focused ultrasound (LIFU) uses ultrasonic pulsations at lower intensities than and is being tested as a reversible precise neuromodulatory technology. Although LIFU-mediated blood-brain barrier (BBB) opening has been explored in detail, no standardized technique for blood-spinal cord (BSCB) established to date. Therefore, this protocol presents method successful BSCB disruption using LIFU sonication rat model, including descriptions of animal preparation, microbubble...
Introduction Despite the advent of immunotherapy as a promising therapeutic, glioblastoma (GBM) remains resistant to using checkpoint blockade due its highly immunosuppressive tumor milieu. Moreover, current anti-PD-1 treatment requires multiple infusions with adverse systemic effects. Therefore, we used PCL:PEG:PCL polymer gel loaded and implanted at site lymph nodes in an attempt maximize targeting inactivated T cells well mitigate unnecessary exposure.Methods Mice orthotopically GL261...
Imaging of spinal cord microvasculature holds great potential in directing critical care management injury (SCI). Traditionally, contrast agents are preferred for imaging the vasculature, which is disadvantageous long-term monitoring injury. Here, we present FlowMorph, an algorithm that uses mathematical morphology techniques to segment non-contrast Doppler-based videos rat cord. Using segmentation, it measures single-vessel parameters such as flow velocity, rate, and radius, with visible...