A5003300494- Retinal Diseases and Treatments
- Ocular Oncology and Treatments
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Toxoplasma gondii Research Studies
- Retinal and Optic Conditions
- Retinal Imaging and Analysis
- Hippo pathway signaling and YAP/TAZ
- Retinal and Macular Surgery
- Ocular Diseases and Behçet’s Syndrome
- Venous Thromboembolism Diagnosis and Management
- Cerebral Venous Sinus Thrombosis
- Drug-Induced Ocular Toxicity
- Ophthalmology and Visual Impairment Studies
- Antiplatelet Therapy and Cardiovascular Diseases
- Herpesvirus Infections and Treatments
- Renal Diseases and Glomerulopathies
- Cell death mechanisms and regulation
- Microtubule and mitosis dynamics
- Renal cell carcinoma treatment
- Cytomegalovirus and herpesvirus research
- melanin and skin pigmentation
- Amyloidosis: Diagnosis, Treatment, Outcomes
- CNS Lymphoma Diagnosis and Treatment
- Complement system in diseases
Lahey Medical Center
2021-2025
Tufts Medical Center
2019-2025
Lahey Hospital and Medical Center
2025
Harvard University
2015-2024
National Eye Institute
2022-2024
National Institutes of Health
2022-2024
Retina Associates
2017-2024
Wills Eye Hospital
2022-2023
Thomas Jefferson University
2022-2023
Massachusetts Eye and Ear Infirmary
2015-2021
Significance Extracellular tissue debris accumulates with aging and in the most prevalent central-vision-threatening eye disorder, age-related macular degeneration (AMD). In this work, we discovered that lysosome-associated membrane protein-2 (LAMP2), a glycoprotein plays critical role lysosomal biogenesis maturation of autophagosomes/phagosomes, is preferentially expressed outermost, neuroepithelial layer retina, retinal pigment epithelium (RPE), contributes to prevention ultrastructural...
Abstract Verteporfin (VP) was first used in Photodynamic therapy, where a non-thermal laser light (689 nm) the presence of oxygen activates drug to produce highly reactive radicals, resulting local cell and tissue damage. However, it has also been shown that can have non-photoactivated effects such as interference with YAP-TEAD complex HIPPO pathway, growth inhibition several neoplasias. More recently, proposed that, another non-light mediated effect VP is formation cross-linked oligomers...
The protozoan parasite Toxoplasma gondii secretes proteins from specialized organelles, the rhoptries, and dense granules, which are involved in modulation of host cell processes. Dense granule protein GRA15 activates nuclear factor kappa B (NF-κB) pathway, plays an important role death, innate immunity, inflammation. Exactly how NF-κB pathway is unknown. Here we show that interacts with tumor necrosis receptor-associated factors (TRAFs), adaptor functioning upstream transcription factor. We...
Abstract Contradictory data have been presented regarding the implication of NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome in age-related macular degeneration (AMD), leading cause vision loss Western world. Recognizing that antibody specificity may explain this discrepancy line with recent National Institutes Health (NIH) guidelines requiring authentication key biological resources, anti-NLRP3 antibodies was assessed to elucidate whether non-immune RPE cells express...
Purpose: The purpose of this study was to determine whether YAP/TAZ activation in uveal melanoma (UM) and the susceptibility cell lines inhibition by verteporfin (VP) is related tumor's genetic background. Methods: Characteristics 144 patients with enucleated UM were analyzed together mRNA expression levels YAP/TAZ-related genes (80 from Cancer Genome Atlas [TCGA] project 64 Leiden, Netherlands). VP administered 92.1, OMM1, Mel270, XMP46, MM28 (UM), CRMM1 CRMM2 (conjunctival melanoma), OCM3...
Choroidal metastasis is the most common intraocular malignancy in adults, commonly presenting later stages of disease and associated with poor prognosis. This first case to describe choroidal involvement as sole initial rare biphasic pulmonary tumor. A 42-year-old white male patient presented emergency department 3 weeks progressive temporal visual field loss left eye (oculus sinister) slight discomfort palpation. At department, he underwent computed tomography angiography brain rule out a...
Purpose: Swept-source optical coherence tomography angiography (SS-OCTA) was used to investigate if the clinical stage of dry age-related macular degeneration (AMD) correlated with global and regional choriocapillaris (CC) perfusion. Methods: In this retrospective, cross-sectional study, 6 × 6-mm SS-OCTA images from eyes early, intermediate, advanced AMD (56 eyes, 41 patients) were analyzed using algorithms described in literature assess flow deficit percentage (FD%) average size. Regions...
To examine the baseline morphological characteristics and alterations in intraretinal microvascular abnormalities (IRMAs) response to anti-vascular endothelial growth factor (anti-VEGF) treatment, documented by optical coherence tomography angiography (OCTA) diabetic eyes.In this retrospective study, IRMAs were evaluated with multimodal imaging (fundus photography, fluorescein angiography, OCTA) treatment-naïve eyes before after anti-VEGF treatment for macular edema (DME) and/or...
Preliminary spectrophotometric and potentiometric studies have shown that hydroxycarbamide or hydroxyurea (HU) can interact with copper(II) [Cu(II)], iron(II) [Fe(II)] Fe(III) ions form complexes, for example, a ratio of 1 HU:1 metal at pH 5. The affinity Cu (log β1 = 3.1) Fe 5) by HU is much lower than the chelating drug deferiprone (L1), which used treatment iron overload. It anticipated under certain conditions high concentrations these such as in transfusional overload, therapeutic,...
The molecular mechanisms involved in induced pluripotent stem cells (iPSCs) generation are poorly understood. cell death machinery of apoptosis-inducing caspases have been shown to facilitate the process iPSCs reprogramming. However, effect other processes, such as programmed necrosis (necroptosis), on induction has not studied. In this study, we investigated role receptor-interacting protein kinase 3 (RIP3), an essential regulator necroptosis, reprogramming mouse embryonic fibroblast (MEFs)...
Age related macular degeneration is the leading cause of blindness in developed world. Although its precise remains elusive, dysfunction retinal pigment epithelium (RPE) and dysregulation complement have been implicated pathogenesis. The goal this study was to evaluate role an AMP-dependent kinase (AMPK) activator, 5-aminoimidazole-4-carboxamide riboside (AICAR), on tumor necrosis factor alpha (TNF-α) induction B (CFB) RPE cells. We found that AICAR inhibited TNF-α-induced CFB expression...
Previous literature assessing ocular hemorrhagic complications of anticoagulant/antiplatelet medications in routine clinical practice is limited. This study evaluates the prevalence spontaneous events associated with anticoagulation/antiplatelet therapy.A retrospective was performed to identify patients taking anticoagulants (rivaroxaban [Xarelto; Janssen Pharmaceuticals, Beerse, Belgium], bivalirudin [Angiomax; The Medicines Company, Parsippany, NJ], lepirudin [Refludan; Bayer HealthCare...
Age-related macular degeneration (AMD) is the most prevalent form of irreversible blindness in developed world. Aging, inflammation and complement dysregulation affecting retinal pigment epithelium (RPE), are considered significant contributors its pathogenesis several evidences have linked tumor necrosis factor alpha (TNF-α) component 3 (C3) with AMD. Acadesine, an analog AMP AMP-activated protein kinase (AMPK) activator, has been shown to cytoprotective effects human clinical trials as...
Abstract Purpose To evaluate the utility of ultrawide-field fluorescein angiography (UWFFA) in patients with anterior uveitis by investigating detection retinal vascular leakage (RVL) and subsequent implications on disease diagnosis management. Study design/materials methods Patients, who were referred to National Eye Institute (NEI) for evaluation underwent UWFFA imaging at initial visit, included this study. The electronic medical records eligible reviewed. images assessed severity...
Timely disease diagnosis is challenging due to increasing burdens and limited clinician availability. AI shows promise in accuracy but faces real-world application issues insufficient validation clinical workflows diverse populations. This study addresses gaps medical downstream accountability through a case on age-related macular degeneration (AMD) severity classification. We designed implemented an AI-assisted diagnostic workflow for AMD, comparing performance with without assistance among...
Purpose To report a case of bilateral exudative retinal detachments and panuveitis in patient with multiple myeloma (MM).Case Report A 54-year-old non-proliferative diabetic retinopathy was referred blurred vision scotomas both eyes (OU). Three months prior to the onset ocular symptoms, he diagnosed systemic MM receiving chemotherapy. Clinical examination revealed best-corrected visual acuities 20/80 OU, rare anterior chamber cell, 2+ vitreous diffuse intraretinal hemorrhages, (RD). Optical...