A5101799487- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Cytokine Signaling Pathways and Interactions
- Genetic diversity and population structure
- Molecular Biology Techniques and Applications
- Machine Learning in Bioinformatics
- Genetic and phenotypic traits in livestock
- Adipose Tissue and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Epigenetics and DNA Methylation
- Carbohydrate Chemistry and Synthesis
- Livestock and Poultry Management
- Identification and Quantification in Food
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Peroxisome Proliferator-Activated Receptors
- Cancer Cells and Metastasis
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Pharmacogenetics and Drug Metabolism
- Fatty Acid Research and Health
- Inflammatory mediators and NSAID effects
Soochow University
2021-2024
Education Department of Heilongjiang Province
2017
Baylor College of Medicine
2008-2015
Harbin Medical University
2015
Third Affiliated Hospital of Harbin Medical University
2015
Texas A&M Health Science Center
2008-2010
North University of China
2010
Texas A&M University
2008-2009
The University of Texas MD Anderson Cancer Center
2009
University of Minnesota
2007
Abstract In breast cancer, steroid receptor coactivator-1 (SRC-1) expression positively correlates with HER2 and poor prognosis. mouse mammary tumor virus–polyoma middle T (PyMT) cancer model, SRC-1 strongly promotes metastasis. However, the molecular targets mechanisms that mediate role of in metastasis are unknown. this study, wild-type (WT) knockout (KO) cell lines were developed from tumors WT/PyMT KO/PyMT mice. WT cells exhibited strong migration invasion capabilities, reduced...
Spermatogenesis, a fundamental process in the male reproductive system, requires series of tightly controlled epigenetic and genetic events germ cells ranging from spermatogonia to spermatozoa. Jmjd1a is key regulator expressed testis. It specifically demethylates mono- di-methylated histone H3 lysine 9 (H3K9me1 H3K9me2) but not tri-methylated H3K9 (H3K9me3). In this study, we generated antibody for immunohistochemistry found was produced pachytene secondary spermatocytes. Disruption gene...
Hydrophilic interaction liquid chromatography (HILIC) is widely used for glycopeptide enrichment in shot-gun glycoproteomics to enhance the signal and minimize ionization competition of peptides. In this work, we have developed a novel hydrophilic material (glycoHILIC) based on glycopeptides peptides provide properties. GlycoHILIC was synthesized by oxidizing cotton then reacting resulting aldehyde with N-terminus or peptide reductive amination. Due large amount carbohydrates amino acids...
Ribonuclease (RNA) modifications can alter cellular function and lead to differential immune responses by acting as discriminators between RNAs from different phyla. RNA glycosylation has recently been observed at the cell surface, its dysregulation in disease may change functions. However, determining which substrates be glycosylated remains explored. Here, we develop a solid-phase chemoenzymatic method (SPCgRNA) for targeting RNAs, specifically recognized. We found N-glycosylation of small...
Urine is thought to provide earlier and more sensitive molecular changes for biomarker discovery than blood. Numerous glycoproteins, peptides, free glycans are present in urine through glomerular filtration of plasma, cell shedding, apoptosis, proteolytic cleavage, exosome secretion. biomarkers have enormous diagnostic potential, the use these a long-standing practice. The non-urological disease from also gaining attention due its non-invasive sample collection ease analysis. Abnormal...
In order to study duck microsatellites, we constructed a library enriched for (CA)n, (CAG)n, (GCC)n and (TTTC)n. A total of 35 pairs primers from these microsatellites were developed used detect polymorphisms in 31 unrelated Peking ducks. Twenty-eight loci polymorphic seven monomorphic. 117 alleles observed microsatellite markers, which ranged 2 14 with an average 4.18 per locus. The frequencies the 0.02 0.98. highest heterozygosity (0.97) was at CAUD019 locus lowest (0.04) CAUD008 locus, 11...
Nuclear receptor coactivator 1 [NCOA1/steroid (SRC)-1] and NCOA3 (SRC-3/AIB1/ACTR) constitute two thirds of the SRC (steroid coactivator) family. Although in vitro experiments have suggested overlapping functions between NCOA1 NCOA3, their vivo functional relationship is poorly understood. In this study, double knockout mice were generated to determine compensatory roles development. NCOA1(-/-) survived normally, whereas most NCOA3(-/-) embryos viable at embryonic d 13.5 (E13.5). contrast,...
Here we demonstrate that reprogramming steroid receptor coactivator-3 (SRC-3) function by changing its posttranslational modification (PTM) code drastically influences systems biology. These findings support the physiological importance of PTMs in directing vivo functions a master coregulator. We previously reported transactivation potential SRC-3 is controlled part PTMs, although this data emanated from vitro studies. To test implications on SRC-3, developed knock-in mouse model containing...
Kdm3b is a JmjC domain-containing histone H3 (H3) demethylase and its physiological functions are largely unknown. In this study, we found that protein highly expressed in multiple cell types the mouse testes, including Leydig cells, Sertoli spermatogonia spermatocytes at different differentiation stages. We also observed epithelial cells of caput epididymis, prostate seminal vesicle. Breeding tests revealed number pups produced by breeding pairs with knockout (Kdm3bKO) males wild type (WT)...
Abstract One major drawback in research of animal mammary gland bioreactors is the low production rate high-expressing transgenic animals due to position effects. To obtain high and stable expression foreign gene, yeast bacterial artificial chromosome have been used as transgene vector recent research. Human lactoferrin a bioactive, versatile protein, has large potential nutritional therapeutic applications. Therefore, recombinant using was studied widely satisfy its requirement. We reported...
The steroid receptor coactivator-3 (SRC-3), also known as AIB1, ACTR, p/CIP and NCOA3, is a transcriptional coactivator for nuclear receptors certain other transcription factors.SRC-3 widely expressed plays important physiological functions pathogenic roles in breast prostate cancers.SRC-3 knockout (SRC-3 -/-) mice display genetic background-dependent embryonic lethality multiple local systemic abnormalities.Since both the partial effects caused by global SRC-3 interfere with downstream...
A chicken bacterial artificial chromosome (BAC) library consisting of 138,240 clones was constructed in vector pBeloBAC11 with genomic DNA isolated from female white-silk chicken. An average insert size 118 kb estimated 452 randomly clones, which indicate the to be approximate 13.34-fold genome coverage. For demonstration probability pick out any unique genes or markers library, 8 single-copy were screened and positive yielded between 2 15 an 11.125, agreement high coverage this library....
In order to study duck microsatellites, we constructed a library enriched for (CA)n, (CAG)n, (GCC)n and (TTTC)n. A total of 35 pairs primers from these microsatellites were developed used detect polymorphisms in 31 unrelated Peking ducks. Twenty-eight loci polymorphic seven monomorphic. 117 alleles observed microsatellite markers, which ranged 2 14 with an average 4.18 per locus. The frequencies the 0.02 0.98. highest heterozygosity (0.97) was at CAUD019 locus lowest (0.04) CAUD008 locus, 11...
Norendoxifen, an active metabolite of tamoxifen, is a potent aromatase inhibitor. Little information available regarding production norendoxifen in vitro. Here, we conducted series kinetic and inhibition studies human liver microsomes (HLMs) expressed P450s to study the metabolic disposition norendoxifen. To validate that was endoxifen, metabolites HLMs incubates endoxifen were measured using HPLC/MS/MS method. further probe specific isoforms involved route, incubated with recombinant (CYP...
<div>Abstract<p>In breast cancer, steroid receptor coactivator-1 (SRC-1) expression positively correlates with HER2 and poor prognosis. In mouse mammary tumor virus–polyoma middle T (PyMT) cancer model, SRC-1 strongly promotes metastasis. However, the molecular targets mechanisms that mediate role of in metastasis are unknown. this study, wild-type (WT) knockout (KO) cell lines were developed from tumors WT/PyMT KO/PyMT mice. WT cells exhibited strong migration invasion...
Supplementary Methods, Figures 1-5 from The Steroid Receptor Coactivator-1 Regulates Twist Expression and Promotes Breast Cancer Metastasis