A5056907066- Lexicography and Language Studies
- Natural Language Processing Techniques
- Linguistics, Language Diversity, and Identity
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- linguistics and terminology studies
- Education, Psychology, and Social Research
- T-cell and B-cell Immunology
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Botany and Plant Ecology Studies
- Estrogen and related hormone effects
- BRCA gene mutations in cancer
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- 14-3-3 protein interactions
- History, Culture, and Society
- Swearing, Euphemism, Multilingualism
- Second Language Acquisition and Learning
- HER2/EGFR in Cancer Research
- Physical Education and Training Studies
- Gender Studies in Language
- Plant Reproductive Biology
- Economic Sanctions and International Relations
Palacký University Olomouc
2012-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2019-2022
Institute of Technology and Business
2022
Institute of Molecular and Translational Medicine
2014-2016
Danish Cancer Society
2014
Institute of Biophysics
2013
Stanford University
2012
Czech Academy of Sciences
2011
Czech Academy of Sciences, Institute of Biophysics
2009
Ghent University
2009
Germline mutations in checkpoint kinase 2 ( CHEK2 ), a multiple cancer‐predisposing gene, increase breast cancer (BC) risk; however, risk estimates differ substantially published studies. We analyzed germline variants 1,928 high‐risk Czech breast/ovarian (BC/OC) patients and 3,360 population‐matched controls (PMCs). For functional classification of VUS, we developed complementation assay human nontransformed RPE1‐ ‐knockout cells quantifying CHK2‐specific phosphorylation endogenous protein...
Abstract The kinase LCK and CD4/CD8 co-receptors are crucial components of the T cell antigen receptor (TCR) signaling machinery, leading to key fate decisions. Despite decades research, roles CD4–LCK CD8–LCK interactions in TCR triggering vivo remain unknown. In this study, we created animal models expressing endogenous levels modified resolve whether how co-receptor-bound drives signaling. We demonstrated that role depends on co-receptor which it is bound. CD8-bound largely dispensable for...
Ag-inexperienced memory-like T (AIMT) cells are functionally unique cells, representing one of the two largest subsets murine CD8+ cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack feral and an unclear relationship AIMT formation during aging represent major barriers for better understanding their biology. We performed thorough characterization mice different genetic background, age, hygienic status by flow cytometry...
Abstract Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and homologous single-copy 2 (Lo2), we have studied stability methylation patterns trans-silencing interactions cell culture regenerated tobacco (Nicotiana tabacum) plants. The posttranscriptionally silenced (PTGS) epiallele Lo1 trans-silences trans-methylates target Lo2 hybrid (Lo1/Lo2 line), while its transcriptionally variant (Lo1E) does not. This...
It has been well established that trans-acting small RNAs guide promoter methylation leading to its inactivation and gene silencing at the transcriptional level (TGS). Here we addressed question of influence locus structure epigenetic modifications target on susceptibility for being paramutated by RNA molecules. Silencing was induced crossing a 35S silencer 271 with two different 35S-driven transgene loci, 2 containing highly expressed single copy 1 an inverted posttranscriptionally silenced...
In plants, silencing is usually accompanied by DNA methylation and heterochromatic histone marks. We studied these epigenetic modifications in different epialleles of 35S promoter (P35S)-driven tobacco transgenes. locus 1, the T-DNA was organized as an inverted repeat, residing neomycin phosphotransferase II reporter gene (P35S-nptII) silenced at posttranscriptional (PTGS) level. Transcriptionally (TGS) were generated trans-acting RNA signals hybrids or a callus culture. PTGS to TGS...
Východiska: Dědičné mutace v genu CHEK2 kódujícím CHK2 proteinkinázu způsobují středně zvýšené riziko vzniku karcinomu prsu (breast cancer -BC) a dalších nádorových onemocnění.Vysoká populační variabilita mutací výskyt vzácných missense variant nejasného významu (variants of unknown clinical significance -VUS) komplikuje odhad rizika onemocnění u nosičů germinálních variant.Soubor pacientů metody: Mutační analýzu CHEK2, vč.analýzy velkých přestaveb, jsme provedli 1 526 vysoce rizikových...
Replication stress (RS) fuels genomic instability and cancer development may contribute to aging, raising the need identify factors involved in cellular responses such stress. Here, we present a strategy for identification of affecting maintenance common fragile sites (CFSs), which are loci that particularly sensitive RS suffer from increased breakage rearrangements tumors. A DNA probe designed match high flexibility island sequence typical commonly expressed CFS (FRA16D) was used as...
There has been a clear increase in the number of e-workers across countries and industries. But experience working from home not same all countries. The purpose this quantitative study was to explore understand managers’ perspectives attitudes towards face-to-display working, namely reactions managers home, their use home-office during ongoing Covid-19 epidemic, advantages disadvantages, productivity, how they cooperated organised teleworking, evaluated reasons that led views on future...
Benamingen van dieren in de Nederlandse enin Tsjechische vaste adjectivische vergelijkingen spiegel geschreven corpora Animal names or zooappellatives constitute an important part of the idioms and proverbs majority natural languages. The Dutch with animal element were described from various perspectives by Kowalska-Szubert 1996. Czech subject studies carried out e.g. Mrhačová et al. 2000, Mrhačová-Kouptsevitch 2004. In this article we compare means corpus research use adjective-based...
Bohemica Olomucensia is published by the Department of Czech Studies in Philosophical Faculty Palacký University Olomouc. The subtitle each number will delimitate its main character (Litteraria, Linguistica, Symposiana, Filologica Juvenilia, Jubilea).
Abstract Antigen-inexperienced memory-like T (AIMT) cells are functionally unique representing one of the two largest subsets murine CD8 + cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack feral and unclear relationship AIMT formation during aging represent major barriers for better understanding their biology. We performed thorough characterization mice different genetic background, age, hygienic status by flow cytometry...
For foreign students, the prepositional object is among most problematic syntactic features of Dutch language, as its form presents strong similarities with adverbial clause, but it has an function. In some cases very difficult to even decide whether phrase function or comparable direct object. this paper compared similar constructions in Czech language. Furthermore, preposition and categorisation are briefly discussed. The ends criteria for Czech-speaking students recognize a such.
West Germanic languages such as Dutch have a temporal system, but typical feature of the Slavonic verb system is aspectuality. Most borrowed or newlyformed verbs in technical sphere are based on Latin, however, language with rather strict system. Typically, mostly imperfective (durative) verbs. This paper examines several and compares their integration into Dutch, Czech Polish. has no morphological forms showing However, aspectuality present, difference there semantic one, between dynamic...