A5021498595- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Animal Disease Management and Epidemiology
- CAR-T cell therapy research
- Alzheimer's disease research and treatments
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Hemoglobin structure and function
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Animal Behavior and Welfare Studies
- Chemokine receptors and signaling
- Animal Virus Infections Studies
- IL-33, ST2, and ILC Pathways
- Growth Hormone and Insulin-like Growth Factors
- Pregnancy and preeclampsia studies
Tokai University
2015-2024
Mabtech (Sweden)
2010
Tarbiat Modares University
2010
Protein Research Foundation
2010
Palmetto Hematology Oncology
2003
National Defense Medical College
2003
Central Institute for Experimental Animals
2003
Tokyo Metropolitan University
1988-1991
Lymphocyte exhaustion was recently recognized as a mechanism of immunosuppression in sepsis. While B cells are known to play pivotal roles bacterial infection and sepsis, changes B-cell-mediated humoral immunity have not been evaluated critically ill septic patients. We aimed investigate caused by defective B-cell function during severe Thirty-three sepsis patients 44 healthy subjects were prospectively enrolled. Blood collected from within 72 h 8 11 after onset measure subtypes, serum...
The highly polymorphic human Major Histocompatibility Complex (MHC) also known as the HLA encodes class I and II genes that are cornerstone of adaptive immune system. Their unique diversity (>25,000 alleles) might affect outcome any transplant, infection, susceptibility to auto-immune diseases. recent rapid development new next generation sequencing (NGS) methods provides opportunity study influence/correlation this high level on allele expression levels in health disease. Here, we describe...
Abstract Objective It is difficult to identify a single causative factor for inflammatory arthritis because of the multifactorial nature disease. This study was undertaken dissect molecular complexity systemic disease, utilizing combined approach mutagenesis and systematic phenotype screening in murine model. Methods In large‐scale N ‐ethyl‐ ‐nitrosourea project, Ali14 mutant mouse strain established dominant inheritance spontaneous swelling inflammation hind paws. Genetic mapping subsequent...
Abstract Sialyl‐Tn (STn) is a tumor‐associated carbohydrate antigen (TACA) rarely observed on healthy tissues. We synthesized two fully synthetic N‐acetyl and N‐propionyl STn trimer (triSTn) vaccines possessing T‐helper epitope TLR2 agonist, since the clustered antigens are highly expressed many cancer cells. Immunization of both in mice induced anti‐triSTn IgG antibodies, which recognized triSTn‐expressing cell lines PANC‐1 HepG2. The triSTn vaccine triSTn‐specific IgGs, while IgGs by were...
Co-assembling vaccines composed of a lipidated HER2-derived antigenic CH401 peptide and either lipophilic adjuvant, Pam3 CSK4 , α-GalCer, or lipid A 506, were evaluated as breast cancer vaccine candidates. This design was aimed to inherit both antigen multivalency antigen-specific immunostimulation properties, observed in reported self-adjuvanting candidates, by using self-assembly adjuvant-conjugated antigens. Under vaccination concentrations, respective adjuvants underwent co-assembly with...
Historically, humoral immunity was considered unimportant in anti-tumor immunity, and the differentiation activity of B cells breast cancer are poorly understood. However, it recently discovered that participate tumor through both antibody production immunosuppressive mechanisms. We analyzed expression B-cell markers detail using fluorescence-activated cell sorting to investigate relationship between subsets etiology. Blood samples were taken from patients healthy donors, peripheral blood...
The common marmoset (Callithrix jacchus) is a New World monkey that used frequently as model for various human diseases. However, detailed knowledge about the MHC still lacking. In this study, we sequenced and annotated total of 854 kb region corresponds to HLA-A/G/F segment (Caja-G/F) between Caja-G1 RNF39 genes. contains 19 class I genes, which 14 are MHC-G (Caja-G) type, 5 MHC-F (Caja-F) type. Six putatively functional Caja-G Caja-F genes (Caja-G1, Caja-G3, Caja-G7, Caja-G12, Caja-G13,...
We report a promising cancer vaccine candidate comprising antigen/adjuvant-displaying enveloped viral replica as novel platform. The artificial capsid, which consists of self-assembled β-annulus peptide conjugated with an HER2-derived antigenic CH401 peptide, was within lipid bilayer containing the lipidic adjuvant α-GalCer. use capsid scaffold enabled precise control its size to ∼100 nm, is generally considered be optimal for delivery lymph nodes. encapsulation anionically charged by...
The common marmoset (Callithrix jacchus) is considered a novel experimental animal model of non-human primates. However, due to antibody unavailability, immunological and pathological studies have not been adequately conducted in various disease models marmoset. Quantitative real-time PCR (qPCR) powerful tool examine gene expression levels. Recent reports shown that selection internal reference housekeeping genes are required for accurate normalization expression. To develop reliable qPCR...
Immunodeficient mice transplanted with human peripheral blood mononuclear cells (PBMCs) are promising tools to evaluate immune responses vaccines. However, these usually develop severe graft-versus-host disease (GVHD), which makes estimation of antigen-specific IgG production after antigen immunization difficult. To in PBMC-transplanted immunodeficient mice, we developed a novel NOD/Shi-scid-IL2rγnull (NOG) mouse strain that systemically expresses the IL-4 gene (NOG-hIL-4-Tg). After PBMC...
Immune checkpoint inhibitors highlight the importance of anticancer immunity. However, their clinical utility and safety are limited by low response rates adverse effects. We focused on progesterone (P4), a hormone produced placenta during pregnancy, because it has multiple biological activities related to immune regulation P4 reversible regulatory function distinct from that stress cortisol, which may drive irreversible suppression promotes T cell exhaustion apoptosis in patients with...
Currently, many peptide vaccines are undergoing clinical studies. Most of these were developed to activate cytotoxic T cells; however, the response is not robust. Unlike vaccines, anti-cancer antibodies based on passive immunity have been approved as a standard treatment. Since more effective in tumor treatment, evidence suggests that limited B cell epitope-based may similar activity. Nevertheless, such intensively primarily because humoral thought be preferable cancer progression. cells...
Progesterone (P4) and glucocorticoid (GC) play crucial roles in the immunoregulation of a mother to accept maintain semi-allogenic fetus. P4 concentration increases during pregnancy becomes much higher placenta than other peripheral tissues, wherein cortisol (COR), most abundant GC strong immunosuppressor, remains uniform throughout rest body. Here, we evaluated effect high-P4 environment on pregnant immunity by comparing it with COR. Naïve T cell proportion increased transiently blood women...
Coccomyxa subellipsoidea KJ (C-KJ) is a green alga with unique immunoregulatory characteristics. Here, we investigated the mechanism underlying modification of T cell function by C-KJ components. The water-soluble extract was fractionated into protein (P) and sugar (S) fractions [acidic (AS), basic (BS), neutral (NS)]. These were used for treatment peripheral blood mononuclear cells stimulated toxic shock syndrome toxin-1. Transcriptome analysis revealed that both P AS enhanced expression...
Progesterone suppresses several ancient pathways in a concentration-dependent manner. Based on these characteristics, progesterone is considered candidate anticancer drug. However, the concentration of used for therapy should be higher than physiological concentration, which makes it difficult to develop progesterone-based drugs. We previously developed liposome-encapsulated (Lipo-P4) with enhanced effects, strongly suppressed triple-negative breast cancer cell proliferation humanized mice....
In the evolution of primates, common marmoset belongs to new world monkey family and is distinct from great ape (which includes humans). this study, we predicted amino acid sequences 30 immunity-related genes compared them with those human mouse. The domain composition each orthologous protein was analyzed by SMART tool found be same among three species. A BLAST search revealed that proteins were 86% identical on average, whereas conservation between mouse or only 60%. This indicates are...
Mast cells (MCs) are developed from hematopoietic progenitor and play an important role in inflammation. Study of the kinetics development accumulation primate MC vivo is crucial for control human inflammatory diseases, as evolution immune system quite rapid inflammation including response considered to be different between mouse human. In present study, we examined progenitors Callithrix jacchus (common marmoset), experimental animal nonhuman primates. Bone marrow were fractionated...