A5100985519- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- Thyroid Cancer Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Fungal and yeast genetics research
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related molecular mechanisms research
- Lung Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Developmental Biology and Gene Regulation
- BRCA gene mutations in cancer
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Medical Imaging and Pathology Studies
- Immune Cell Function and Interaction
- Colorectal Cancer Screening and Detection
- Cervical Cancer and HPV Research
- IL-33, ST2, and ILC Pathways
Sinotech Genomics (China)
2019-2024
The First Affiliated Hospital, Sun Yat-sen University
2019
Sun Yat-sen University
2019
Zhejiang University
2017-2018
Stowers Institute for Medical Research
2011-2016
University at Albany, State University of New York
2007-2014
Wadsworth Center
2014
New York State Department of Health
2014
University of Toronto
2010
Abstract Early detection has the potential to reduce cancer mortality, but an effective screening test must demonstrate asymptomatic years before conventional diagnosis in a longitudinal study. In Taizhou Longitudinal Study (TZL), 123,115 healthy subjects provided plasma samples for long-term storage and were then monitored occurrence. Here we report preliminary results of PanSeer, noninvasive blood based on circulating tumor DNA methylation, TZL from 605 individuals, 191 whom later...
The packaging of eukaryotic DNA into chromatin has profound consequences for gene regulation, as well other transactions such recombination, replication and repair. Understanding how this is determined consequently a pressing problem in molecular genetics. sequence, remodelers transcription factors affect structure, but the scope these influences on genome-wide nucleosome occupancy patterns remains uncertain. Here, we use high resolution tiling arrays to examine contributions two general...
Increasing evidences have suggested that natural killer (NK) cells in the tumor microenvironment are involved regulation of cancer development. However, potential biological roles and regulatory mechanisms NK pancreatic (PC) remain unclear. Co-culture system with PC is used to test ability cell proliferation, migration invasion both
Mediator is a large, multisubunit complex that essential for transcription of mRNA by RNA Pol II in eukaryotes and believed to bridge transcriptional activators the general machinery. However, several recent studies suggest requirement during activation not universal, but rather activator dependent, may be indirect some genes. Here we have investigated association with constitutively transcribed genes yeast comparing strain harbors temperature-sensitive mutation an subunit, Srb4, its...
Histone modifications are frequently used as markers for enhancer states, but how to interpret states in the context of embryonic development is not clear. The poised signature, involving H3K4me1 and low levels H3K27ac, has been reported mark inactive enhancers that future activation. However, activation always observed, alternative reasons widespread occurrence this signature have investigated. By analyzing during dorsal-ventral (DV) axis formation Drosophila embryo, we find specifically...
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) has the lowest overall survival rate primarily due to late onset of symptoms and rapid progression. Reliable accurate tests for early detection are lacking. We aimed develop a noninvasive test PDAC by capturing circulating tumour DNA (ctDNA) methylation signature in blood. Methods Genome-wide profiles were generated from nonmalignant tissues plasma. Methylation haplotype blocks (MHBs) examined discover de novo markers. They combined...
Thyroid nodule (TN) patients in China are subject to overdiagnosis and overtreatment. The implementation of existing technologies such as thyroid ultrasonography has indeed contributed the improved diagnostic accuracy TNs. However, a significant issue persists, where many undergo unnecessary biopsies, with malignant nodules (MTNs) advised surgery therapy.
Transcription by RNA polymerase II (Pol II) in eukaryotes requires the Mediator complex, and often involves chromatin remodeling histone eviction at active promoters. Here we address role of recruitment Swi/Snf complex its role, along with components preinitiation (PIC), inducible constitutively promoters budding yeast Saccharomyces cerevisiae. We show that to induced CHA1 promoter, as well association several promoters, depends on but is independent MET2 MET6 genes. Although transcriptional...
Accurately distinguishing between benign thyroid nodules (BTNs) and papillary cancers (PTCs) with current conventional methods poses a significant challenge.
Accurate differentiation between malignant and benign pulmonary nodules, especially those measuring 5-10 mm in diameter, continues to pose a significant diagnostic challenge. This study introduces novel, precise approach by integrating circulating cell-free DNA (cfDNA) methylation patterns, protein profiling, computed tomography (CT) imaging features enhance the classification of nodules.
Mediator complex is essential for transcription by RNA polymerase II in eukaryotes. Although chromatin remodeling an integral part of transcriptional activation at many promoters, whether required this function has not been determined. Here we have used the yeast CHA1 gene to study role and recruitment machinery. We show immunoprecipitation that subunits are recruited induced promoter. Inactivation 37 °C harboring srb4-138 (med17) ts mutation severely reduces prevents promoter Med18/Srb5,...
Some drugs that target molecular pathways are available for the targeted treatment of lung cancer. Multiple tests needed to detect status known targets determine whether patients can respond drugs. An integrated platform various gene alteration detection including both mutations and rearrangements is necessary patients, especially those without enough tissue.In our study, detections EGFR mutations, ALK rearrangement, ROS1 alterations other nine important cancer-related genes were into a...
An accurate and reproducible next-generation sequencing platform is essential to identify malignancy-related abnormal DNA methylation changes translate them into clinical applications including cancer detection, prognosis, surveillance. However, high-quality has been challenging because poor sequence diversity of the bisulfite-converted libraries severely impairs quality yield. In this study, we tested MGISEQ-2000 Sequencer's capability with a published non-invasive pancreatic detection...
We aimed to examine the therapeutic potential of polysaccharide H-1-2, a bioactive component Pseudostellaria heterophylla, against pancreatic cancer, as well demonstrate underlying molecular mechanisms. Invasion and migration cells treated with H-1-2 were evaluated. A xenograft tumor mouse model was established assess effect on growth. Expression levels hypoxic inducible factor-1α (HIF1α) anterior gradient 2 (AGR2) measured in after treatment. Luciferase report chromatin immunoprecipitation...
Abstract Context Follicular thyroid carcinoma (FTC) is the second most common type of and must be pathologically distinguished from benign follicular adenoma (FA). Additionally, clinical assessment tumors with uncertain malignant potential (TT-UMP) demands effective indicators. Objective We aimed to identify discriminating DNA methylation markers between FA FTC. Methods patterns were investigated in 33 FTC samples using reduced representation bisulfite sequencing haplotype block–based...
Preoperative evaluation of microvascular invasion (MVI) in patients with hepatocellular carcinoma (HCC) is important for surgical strategy determination. We aimed to develop and establish a preoperative predictive model MVI status based on DNA methylation markers.A total 35 HCC tissues the matched peritumoral normal liver as well corresponding patients' plasma samples 24 healthy were used genome-wide sequencing subsequent haplotype block (MHB) analysis. Predictive models constructed selected...
330 Background: Gastrointestinal (GI) cancers totally account for more than one third of the cancerous deaths, yet there is no cost-effective blood-based assay early detection GI cancers. We sought to develop GutSeer, a noninvasive test based on cell-free DNA (cfDNA) methylation and fragmentation signatures derived from single targeted sequencing panel, localization five major cancers, including colorectal (CC), gastric (GC), liver (LC), esophageal (EC), pancreatic cancer (PC). Methods: A...
The histone H3 amino terminus, but not that of H4, is required to prevent the constitutively bound activator Cha4 from remodeling chromatin and activating transcription at CHA1 gene in Saccharomyces cerevisiae. Here we show neither modifiable lysine residues nor any specific region tail for repression CHA1. We then screened mutations cause derepression uninduced promoter identified six mutants, three which are also temperature-sensitive mutants four exhibit a sin(-) phenotype. Histone mutant...