A5100330841- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Cancer, Lipids, and Metabolism
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Acupuncture Treatment Research Studies
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Biosensors and Analytical Detection
- Inflammasome and immune disorders
- Advanced Fluorescence Microscopy Techniques
- Lipid metabolism and biosynthesis
- Neurological Disease Mechanisms and Treatments
- Cancer Mechanisms and Therapy
- Phytochemistry and Bioactive Compounds
- Inflammatory Biomarkers in Disease Prognosis
- Healthcare and Venom Research
- Medicinal Plants and Bioactive Compounds
- Nanoplatforms for cancer theranostics
Peking University
2021-2025
Third Affiliated Hospital of Sun Yat-sen University
2023-2025
Sun Yat-sen University
2023-2025
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2024
Tsinghua University
2023-2024
Suzhou Traditional Chinese Medicine Hospital
2024
Nanjing University
2024
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2024
National Clinical Research
2024
Ningxia Medical University
2024
This work presented a point-of-care (POC) photoelectrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on portable electrochemical system by using CRISPR-Cas12a trans-cleaving G-quadruplex biorecognition/amplification and hollow In2O3-In2S3-modified screen-printed electrode (In2O3-In2S3/SPE) as photoactive material. G-quadruplexes were capable biocatalytic precipitation (H2O2-mediated 4-chloro-1-naphthol oxidation) In2O3-In2S3/SPE surface, resulting in weakened...
Abstract Expanding mitochondrial base editing tools with broad sequence compatibility is of high need for both research and therapeutic purposes. In this study, we identify a DddA homolog from Simiaoa sunii (Ddd_Ss) which can efficiently deaminate cytosine in D C context double-stranded DNA (dsDNA). We successfully develop Ddd_Ss-derived editors (DdCBE_Ss) introduce mutations at multiple (mtDNA) loci including disease-associated mtDNA previously inaccessible G context. Finally, by...
Background/Aims: The pro-angiogenic factors vascular endothelial growth factor (VEGF) and angiopoietins (Angs) play a prominent role in synovial angiogenesis, an early critical event the pathogenesis of rheumatoid arthritis (RA). Interleukin (IL)-35 is anti-inflammatory cytokine that attenuates collagen-induced arthritis, however, mechanisms involved are not fully understood. Methods: effects IL-35 on cell migration, adhesion, tube formation were examined using human umbilical vein cells...
Nondestructive sequencing of RNA from live cells is essential for monitoring and understanding dynamic biological processes. However, most existing methods rely on cell lysis or fixation, limiting their applicability longitudinal studies. Here, we introduce POND-seq (Protein nanocage-empOwered Non-Destructive sequencing), a novel approach that employs secretory protein nanocages fused with RNA-binding proteins (RBPs) to capture the RBP-RNA interactome transcriptome in cells. reliably...
<title>Abstract</title> Nondestructive sequencing of RNA from live cells is essential for monitoring and understanding dynamic biological processes. However, most existing methods rely on cell lysis or fixation, limiting their applicability longitudinal studies. Here, we introduce POND-seq (Protein nanocage-empOwered Non-Destructive sequencing), a novel approach that employs secretory protein nanocages fused with RNA-binding proteins (RBPs) to capture the RBP-RNA interactome transcriptome in...
Abstract Background Degrons are short linear motifs, bound by E3 ubiquitin ligase to target protein substrates be degraded the ubiquitin-proteasome system. Mutations leading deregulation of degron functionality disrupt control abundance due mistargeting proteins destined for degradation and often result in pathologies. Targeting degrons small molecules also emerges as an exciting drug design strategy upregulate expression specific proteins. Despite their essential function disease...
Resistance to lenvatinib limits the effectiveness of targeted treatments for HCC. However, exact mechanism behind this resistance remains elusive. Current research suggests that circular RNA (circRNA) is pivotal in mediating drug during treatments. To investigate influence circRNA on HCC progression and its lenvatinib. We identified crucial hsa_circ_0005711 (circPIK3C3) through bioinformatics. Study (in-vitro in-vivo) expression circPIK3C3 (measured by qRT-PCR) association with progress...
Incorporation of structurally novel noncanonical amino acids (ncAAs) into proteins is valuable for both scientific and biomedical applications. To expand the structural diversity available ncAAs to reduce burden chemically synthesizing them, we have developed a general simple biosynthetic method genetically encoding recombinant by feeding cells with economical commercially or synthetically accessible aromatic thiols. We demonstrate that nearly 50 diverse array structures can be...
Abstract Background Pyroptosis is a type of programmed cell death mediated by the gasdermin family. Gasdermin B (GSDMB), as member family, can promote occurrence pyroptosis. However, correlations GSDMB expression in colorectal cancer with clinicopathological predictors, immune microenvironment, and prognosis are unclear. Methods Specimens from 267 cases were analyzed immunohistochemistry to determine expression, CD3 + , CD4 CD8 T lymphocytes, CD20 CD68 macrophages, S100A8 cells. cells was...
This work reported a photoelectrochemical bioassay for prostate-specific antigen detection using the lanthanide-doped bismuth ferrite/reduced graphene oxide/tungsten oxide nanohybrids.
Long non-coding RNAs have been reported to play a crucial role in tumor progression hepatocellular carcinoma (HCC).Lnc-ZEB2-19 has validated be deficiently expressed HCC.However, the capabilities and underlying mechanisms of lnc-ZEB2-19 remain uncertain.In this study, we verified that downregulation was prevalent HCC significantly correlated with unfavorable prognosis.Further vitro vivo notably inhibited proliferation, metastasis, stemness, lenvatinib resistance (LR) cells.Mechanistically,...
Colorectal cancer (CRC) ranks as the third most prevalent malignancy worldwide. Despite gradual expansion of therapeutic options for CRC, its clinical management remains a formidable challenge. And, because current dearth technical means early CRC screening, patients are diagnosed at an advanced stage. Therefore, it is imperative to develop novel diagnostic and tools this disease. N6-methyladenosine (m6A), predominant RNA modification in eukaryotes, can be recognized by m6A-specific...
Given its high morbidity, disability, and mortality rates, ischemic stroke (IS) is a severe disease posing substantial public health threat. Although early thrombolytic therapy effective in IS treatment, the limited time frame for administration presents formidable challenge. Upon occurrence, triggers an cascade response, inducing brain to generate endogenous protective mechanisms against excitotoxicity inflammation, among other pathological processes. Stroke patients often experience...
Pyroptosis is a type of cell death that causes an immune reaction. Gasdermin D (GSDMD), as executor pyroptosis, has become attractive target in cancer research. However, the clinical significance GSDMD expression different subcellular locations remains unclear.GSDMD was detected by immunohistochemistry 178 cases colorectal with follow-up information. General data and information on systemic inflammatory indicators were collected from case records, clinicopathological parameters reviewed...
Phenazine compounds are widely used in agricultural control and the medicine industry due to their high inhibitory activity against pathogens antitumor activity. The green sustainable method of synthesizing phenazine through microbial fermentation often requires a complex culture medium containing tryptone yeast extract, its cost is relatively high, which greatly limits large-scale industrial production by fermentation. aim this study was develop cost-effective minimal for efficient...
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The increasing use of probabilistic algorithms from statistics and machine learning for data analytics presents new challenges opportunities the design computing systems. One important class is Markov Chain Monte Carlo (MCMC) sampling, which can be used on a wide variety applications in Bayesian Inference. However, this iterative algorithm inefficient practice today's processors, especially problems with high dimensionality complex structure. source inefficiency generating samples...
Acupuncture has a positive effect in the treatment of ischemic stroke (IS). A number studies have confirmed that role acupuncture IS, which is closely related to its functions regulating mitochondrial functions. In present article, we review mechanisms underlying improvement mitochondria IS from 4 aspects： 1) protecting structure integrity, 2) regulative on functional activities, including energy metabolism, reducing oxidative stress, suppressing calcium overload, and membrane potential...