A5101555720- Neuroinflammation and Neurodegeneration Mechanisms
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Neurological Disease Mechanisms and Treatments
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Sirtuins and Resveratrol in Medicine
- Immune Response and Inflammation
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics, phytochemicals, and oxidative stress
- Connexins and lens biology
- Phytochemicals and Antioxidant Activities
- Alzheimer's disease research and treatments
- Curcumin's Biomedical Applications
- Circadian rhythm and melatonin
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Medicinal Plants and Bioactive Compounds
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Natural Antidiabetic Agents Studies
Allen Institute for Brain Science
2018-2025
Icahn School of Medicine at Mount Sinai
2018-2025
Kaohsiung Medical University
2019-2024
Kaohsiung Medical University Chung-Ho Memorial Hospital
2019-2024
Northwestern University
2023-2024
James J. Peters VA Medical Center
2021-2023
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2023
University of Palermo
2023
Geriatric Research Education and Clinical Center
2021-2023
China Medical University
2010-2020
Abstract In humans, traumatic social experiences can contribute to psychiatric disorders 1 . It is suggested that trauma impairs brain reward function such behaviour no longer rewarding, leading severe avoidance 2,3 rodents, the chronic defeat stress (CSDS) model has been used understand neurobiology underlying susceptibility versus resilience following trauma, yet little known regarding its impact on 4,5 Here we show that, CSDS, a subset of male and female mice, termed susceptible (SUS),...
Abstract Psychosocial stress has profound effects on the body, including immune system and brain 1,2 . Although a large number of pre-clinical clinical studies have linked peripheral alterations to stress-related disorders such as major depressive disorder (MDD) 3 , underlying mechanisms are not well understood. Here we show that expression circulating myeloid cell-specific proteinase, matrix metalloproteinase 8 (MMP8), is increased in serum humans with MDD stress-susceptible mice following...
Abstract Single-cell nanopore sequencing of full-length mRNAs transforms single-cell multi-omics studies. However, challenges include high errors and dependence on short-reads and/or barcode whitelists. To address these, we develop scNanoGPS to calculate same-cell genotypes (mutations) phenotypes (gene/isoform expressions) without short-read nor whitelist guidance. We apply onto 23,587 long-read transcriptomes from 4 tumors 2 cell-lines. Standalone, deconvolutes error-prone long-reads into...
Microglial activation has been widely demonstrated to mediate inflammatory processes that are crucial in several neurodegenerative disorders. Pharmaceuticals can deliver direct inhibitory effects on microglia therefore considered as a potential strategy counter balance progression. Caffeic acid phenethyl ester (CAPE), natural phenol honeybee propolis, is known possess antioxidant, anti-inflammatory and anti-microbial properties. Accordingly, the current study intended probe of CAPE by using...
Background/Objective Nicardipine is a calcium channel blocker that has been widely used to control blood pressure in severe hypertension following events such as ischemic stroke, traumatic brain injury, and intracerebral hemorrhage. However, accumulating evidence suggests inflammatory processes the central nervous system are mediated by microglial activation play important roles neurodegeneration, effect of nicardipine on remains unresolved. Methodology/Principal Findings In present study,...
Malignant gliomas are associated with high morbidity and mortality because they highly invasive into surrounding brain tissue, making complete surgical resection impossible. Osteopontin is abundantly expressed in the involved cell adhesion, migration, invasion. The aim of present study was to investigate mechanisms glioma migration. Migration invasion activity were determined by transwell wound-healing assays. Gene protein expressions analyzed reverse transcription-PCR, real time-PCR,...
Major Depressive Disorder (MDD) is a devastating, multifactorial disease with limited pharmacological treatment options. Patients MDD exhibit alterations in their dopamine (DA) signaling pathways through the midbrain ventral tegmental area (VTA). A similar observation also detected preclinical models of stress - mice behavioral and physiological impairments following chronic social defeat (CSDS). Prior studies demonstrate that CSDS-susceptible have increased VTA DA neuronal excitability,...
We recently reported that peptidoglycan (PGN), a cell wall component of the Gram-positive bacterium, induces NF-κB activation and microglia activation. However, PGN-regulated AP-1 cytokine expression in remains unclear. This study investigated how PGN influences signaling pathway involved IL-6 production microglia. mRNA protein level up-regulation were increased by concentration- time-dependent manner. In addition, toll-like receptor-2 (TLR2) expression, but not TLR4 receptor up-regulation....
Increasing evidence suggests that inflammatory processes in the central nervous system are mediated by microglial activation play a key role neurodegeneration. Fisetin, plant flavonol commonly found fruits and vegetables, is frequently added to nutritional supplements due its antioxidant properties. In present study, treatment with fisetin inhibited cell migration ROS (reactive oxygen species) production. Treatment also effectively LPS plus IFN-γ-induced nitric oxide (NO) production,...
Brain-derived neurotrophic factor (BDNF) is a potent that has been shown to affect cancer cell metastasis and migration. In the present study, we investigated mechanisms of BDNF-induced migration in colon cells. The migratory activities two lines, HCT116 SW480, were found be increased presence human BDNF. Heme oxygenase-1 (HO)-1 known involved development progression tumors. However, molecular underlie HO-1 regulation remain unclear. Expression protein mRNA response BDNF stimulation....
Endometriosis is considered a high risk factor for the development of ovarian carcinoma, including clear cell and endometrioid malignancies. The mechanism by which endometriosis-associated cancer (EAOC) avoids anti-tumor immune surveillance macrophages remains unclear, but CD47 very important checkpoint macrophage phagocytosis. Therefore, we collected 36 clinical samples detected protein profile immunohistochemistry analyzed correlation with pathological features using statistical software....
Clinical studies have revealed a high comorbidity between autoimmune diseases and psychiatric disorders, including major depressive disorder (MDD). However, the mechanisms connecting autoimmunity depression remain unclear. Here, we aim to identify processes by which stress impacts adaptive immune system implications of such responses depression. To examine this relationship, analyzed antibody in chronic social defeat (CSDS) model mice, clinical samples from patients with MDD. We show that...
Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in adult central nervous system. Temozolomide (TMZ) has been considered to be one effective chemotherapeutic agents prolong survival patients with glioblastoma. Many glioma cells develop drug-resistance against TMZ that mediated by increasing O-6-methylguanine-DNA methyltransferase (MGMT) levels. The expression connexin 43 was increased resistant U251 subline compared parental cells....
In study, the expression patterns and functional differences between an original glioma cell population (U251 U87) sublines (U251-P10, U87-P10) that were selected to be migration-prone investigated. The expressions levels of VEGF intracellular adhesion molecule-1 (ICAM-1) increased in as well samples from patients with high-grade when compared those low-grade glioma. addition, cells showed oncogenic microRNA. miR-21, which was also associated more advanced clinical pathological stages...
Angiogenesis, a prominent feature of pathology, is known to be guided by factors secreted living cells around lesion. Although many are disrupted in response injury, the relevance degenerating pathological angiogenesis unclear. Here, we show that release lactate dehydrogenase A (LDHA) from neurons drives central nervous system (CNS) angiogenesis. Silencing neuronal LDHA expression suppressed experimental autoimmune encephalomyelitis (EAE)- and controlled cortical impact-induced lesions....