A5020376244- Malaria Research and Control
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Parasites and Host Interactions
- Animal Virus Infections Studies
- Child Nutrition and Water Access
- Travel-related health issues
- Poverty, Education, and Child Welfare
- Computational Drug Discovery Methods
- Hepatitis C virus research
- Global Maternal and Child Health
- Hemoglobinopathies and Related Disorders
- vaccines and immunoinformatics approaches
- Congenital Anomalies and Fetal Surgery
Manhiça Health Research Centre
2022-2025
Barcelona Institute for Global Health
2025
Instituto Nacional de Saúde
2019-2022
Eduardo Mondlane University
2020-2021
Abstract Targeted amplicon sequencing is a powerful and efficient tool to interrogate the P . falciparum genome generate actionable data from infections complement traditional malaria epidemiology. For maximum impact, genomic tools should be multi-purpose, robust, sensitive reproducible. We developed, characterized, implemented MAD 4 HatTeR, an panel based on Multiplex Amplicons for Drug, Diagnostic, Diversity, Differentiation Haplotypes using Resequencing, along with bioinformatic pipeline...
Abstract Mozambique is one of the four African countries which account for over half all malaria deaths worldwide, yet little known about parasite genetic structure in that country. We performed P. falciparum amplicon and whole genome sequencing on 2251 malaria-infected blood samples collected 2015 2018 seven provinces to genotype antimalarial resistance markers interrogate population using genome-wide microhaplotyes. Here we show only resistance-associated observed at frequencies above 5%...
Targeted amplicon sequencing is a powerful and efficient tool for interrogating the Plasmodium falciparum genome, generating actionable data from infections to complement traditional malaria epidemiology. For maximum impact, genomic tools should be multi-purpose, robust, sensitive, reproducible. We developed, characterized, implemented MAD4HatTeR, an panel based on Multiplex Amplicons Drug, Diagnostic, Diversity, Differentiation Haplotypes using Resequencing, along with bioinformatic...
Group A rotavirus (RVA) remains the most important etiological agent associated with severe acute diarrhea in children. Rotarix® monovalent vaccine was introduced into Mozambique’s Expanded Program on Immunization September 2015. In present study, we report diversity and prevalence of genotypes, pre- (2012–2015) post-vaccine (2016–2019) introduction Mozambique, among diarrheic children less than five years age. Genotyping data were analyzed for sentinel sites periods indicated. The primary...
Introduction Genomic data constitute a valuable adjunct to routine surveillance that can guide programmatic decisions reduce the burden of infectious diseases. However, genomic capacities remain low in Africa. This study aims operationalise functional malaria molecular system Mozambique for guiding control and elimination. Methods analyses prospective seeks generate Plasmodium falciparum genetic (1) monitor markers drug resistance deletions rapid diagnostic test targets; (2) characterise...
Abstract Background Mozambique has a high burden of group A rotavirus (RVA) infection and chronic undernutrition. This study aimed to determine the frequency potential risk factors for RVA in undernourished children under 5 years old with diarrhoea Mozambique. Methods The analysis was conducted using data from March 2015 December 2017, regarding at least one type Anthropometric measures were used calculate indices weight-for-age, weight-for-height height-for-age through Z-Scores. results...
Mozambique introduced the monovalent rotavirus vaccine (Rotarix®, GSK Biologicals, Rixensart, Belgium) in September 2015. Previous analysis, showed that Nampula province continues reporting a high frequency of Rotavirus A (RVA) infection and emergence G9P[6], G9P[4] G3P[4] genotypes. This analysis aimed to determine RVA frequency; risk factors; genotype distribution by vaccination status age between pre- post-vaccine periods children under-five years old with diarrhea Nampula....
Mozambique introduced the Rotarix® vaccine (GSK Biologicals, Rixensart, Belgium) into National Immunization Program in September 2015. Although G1P[8] was one of most prevalent genotypes between 2012 and 2017 Mozambique, no complete genomes had been sequenced to date. Here we report whole genome sequence analysis for 36 strains using an Illumina MiSeq platform. All exhibited a Wa-like genetic backbone (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Phylogenetic showed that Mozambican clustered closely...
The World Health Organization’s systems framework shows that service delivery is key to addressing pressing health needs. Inadequate healthcare and the lack of services are factors associated with undernutrition diarrhea in children under five, two conditions high morbi-mortality rates Mozambique. aim analysis was determine readiness score nutrition for five influence malaria HIV (Human Immunodeficiency Virus) on these services. A total 1644 public facilities Mozambique were included from...
This study presents whole genomes of seven bovine rotavirus strains from South Africa and Mozambique. Double-stranded RNA, extracted stool samples without prior adaptation to cell culture, was used synthesise cDNA using a self-annealing anchor primer ligated dsRNA random hexamers. The subsequently sequenced an Illumina MiSeq platform genome amplification. All exhibited bovine-like artiodactyl constellations (G10/G6-P[11]/P[5]-I2-R2-C2-M2-A3/A11/A13-N2-T6-E2-H3). Phylogenetic analysis...
<ns3:p>Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Program (2023-2030) for malaria control and elimination. Methods analyses prospective genomic <ns3:italic>P. falciparum</ns3:italic> genetic data monitor diagnostic failures due <ns3:italic>pfhrp2/3</ns3:italic> deletions...
Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Programme (2023–2030) for malaria control and elimination. Methods analyses prospective genomic Plasmodium falciparum genetic data monitor diagnostic failures due pfhrp2/3 deletions markers drug resistance, characterise transmission...
<title>Abstract</title> Background Rapid diagnostic tests (RDTs) based on the detection of <italic>P. falciparum</italic> histidine rich protein 2 (<italic>Pf</italic>HRP2) are widely used for in Africa. However, deletions <italic>pfhrp2</italic> and <italic>pfhrp3</italic> genes can lead to false negative test results compromise appropriate case management. Due high burden malaria Mozambique, it is crucial monitor potential emergence parasites with <italic>pfhrp2/3</italic> gene country....
Rapid diagnostic tests (RDTs) based on the detection of Plasmodium falciparum histidine rich protein 2 (PfHRP2) are widely used for P. in Africa. However, deletions pfhrp2 and pfhrp3 genes can lead to false negative test results compromise appropriate case management. Due high burden malaria Mozambique, it is crucial monitor potential emergence parasites with pfhrp2/3 gene country. The presence was assessed during 2023 transmission season 34 health facilities from 9 districts across 6...