A5077396762- Malaria Research and Control
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Computational Drug Discovery Methods
- Horticultural and Viticultural Research
- HIV/AIDS drug development and treatment
- Hemoglobinopathies and Related Disorders
- Global Maternal and Child Health
- Travel-related health issues
- Hepatitis C virus research
- Plant Physiology and Cultivation Studies
- Biochemical and Structural Characterization
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Insect and Pesticide Research
- vaccines and immunoinformatics approaches
- Phytase and its Applications
- Insect-Plant Interactions and Control
- Plant Stress Responses and Tolerance
- Child Nutrition and Water Access
- Plant-Microbe Interactions and Immunity
Manhiça Health Research Centre
2022-2025
Universidade Nova de Lisboa
2021-2024
University of Lisbon
2018
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compare the genetic structure parasite populations sampled from 289 ANC users 93 children community Mozambique between 2015 2019. Samples are amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...
Abstract Targeted amplicon sequencing is a powerful and efficient tool to interrogate the P . falciparum genome generate actionable data from infections complement traditional malaria epidemiology. For maximum impact, genomic tools should be multi-purpose, robust, sensitive reproducible. We developed, characterized, implemented MAD 4 HatTeR, an panel based on Multiplex Amplicons for Drug, Diagnostic, Diversity, Differentiation Haplotypes using Resequencing, along with bioinformatic pipeline...
Abstract Mozambique is one of the four African countries which account for over half all malaria deaths worldwide, yet little known about parasite genetic structure in that country. We performed P. falciparum amplicon and whole genome sequencing on 2251 malaria-infected blood samples collected 2015 2018 seven provinces to genotype antimalarial resistance markers interrogate population using genome-wide microhaplotyes. Here we show only resistance-associated observed at frequencies above 5%...
While the
Targeted amplicon sequencing is a powerful and efficient tool for interrogating the Plasmodium falciparum genome, generating actionable data from infections to complement traditional malaria epidemiology. For maximum impact, genomic tools should be multi-purpose, robust, sensitive, reproducible. We developed, characterized, implemented MAD4HatTeR, an panel based on Multiplex Amplicons Drug, Diagnostic, Diversity, Differentiation Haplotypes using Resequencing, along with bioinformatic...
Introduction Genomic data constitute a valuable adjunct to routine surveillance that can guide programmatic decisions reduce the burden of infectious diseases. However, genomic capacities remain low in Africa. This study aims operationalise functional malaria molecular system Mozambique for guiding control and elimination. Methods analyses prospective seeks generate Plasmodium falciparum genetic (1) monitor markers drug resistance deletions rapid diagnostic test targets; (2) characterise...
Malaria remains one of the most serious public health problems in sub-Saharan Africa and Mozambique is world's fourth largest contributor, with 4.7% disease cases 3.6% total deaths due to malaria. Its control relies on fight against vector treatment confirmed anti-malarial drugs. Molecular surveillance an important tool for monitoring spread drug resistance.A cross-sectional study recruited 450 participants malaria infection detected by Rapid Diagnostic Tests, from three different sites...
Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Programme (2023–2030) for malaria control and elimination. Methods analyses prospective genomic Plasmodium falciparum genetic data monitor diagnostic failures due pfhrp2/3 deletions markers drug resistance, characterise transmission...
Malaria is one of the 'big three' killer infectious diseases, alongside tuberculosis and HIV. In non-endemic areas, malaria may occur in travelers who have recently been to or visited endemic regions. The number imported cases Portugal has increased recent years, mostly due close relationship with community Portuguese language countries. Samples were collected from malaria-infected patients attending Centro Hospitalar Lisboa Ocidental (CHLO) outpatient clinic Instituto de Higiene e Medicina...
<ns3:p>Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Program (2023-2030) for malaria control and elimination. Methods analyses prospective genomic <ns3:italic>P. falciparum</ns3:italic> genetic data monitor diagnostic failures due <ns3:italic>pfhrp2/3</ns3:italic> deletions...
<title>Abstract</title> Background Rapid diagnostic tests (RDTs) based on the detection of <italic>P. falciparum</italic> histidine rich protein 2 (<italic>Pf</italic>HRP2) are widely used for in Africa. However, deletions <italic>pfhrp2</italic> and <italic>pfhrp3</italic> genes can lead to false negative test results compromise appropriate case management. Due high burden malaria Mozambique, it is crucial monitor potential emergence parasites with <italic>pfhrp2/3</italic> gene country....
Rapid diagnostic tests (RDTs) based on the detection of Plasmodium falciparum histidine rich protein 2 (PfHRP2) are widely used for P. in Africa. However, deletions pfhrp2 and pfhrp3 genes can lead to false negative test results compromise appropriate case management. Due high burden malaria Mozambique, it is crucial monitor potential emergence parasites with pfhrp2/3 gene country. The presence was assessed during 2023 transmission season 34 health facilities from 9 districts across 6...
Subtilases are serine peptidases involved in several plant biological functions, however one of their most important participation is the response to biotic and abiotic stresses. have been linked hormone-associated signalling, like jasmonic acid (JA) pathway, that particularly related with defence responses against necrotrophic fungus herbivores. In grapevine, recent studies implicated JA pathway Plasmopara viticola, a biotrophic oomycete. Our more results showed an increased expression...
The reduction of pesticide use to control pests and diseases is one modern agriculture’s demands. In the grapevine-Plasmopara viticola pathosystem, repeated preventive applications fungicides occur during vegetative period. identification specific mechanisms or molecules with bioactive fungicide properties may lead new disease strategies. Following an omics-based system biology approach we were able identify both subtilisin-like proteases lipid associated signalling events as key players on...
Background: Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We aimed to compare the genetic structure ANC community parasite populations, including changes following an elimination program. Methods: 289 attendees 93 children from were sampled low-to-moderate transmission Maputo province, Mozambique, between 2015 2019. Samples amplicon sequenced targeting 165...
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compared the genetic structure parasite populations sampled from 289 ANC attendees 93 children community Mozambique between 2015 2019. Samples were amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...
Abstract Malaria remains one of the most serious public health problem in sub-Saharan Africa and Mozambique is world's fourth largest contributor, with 4.7% disease cases 3.6% total deaths due to malaria. Its control relies on fight against vector treatment confirmed antimalarial drugs. Molecular surveillance important tool for monitoring spread drug resistance. A cross-sectional study recruited 450 participants malaria infection detected by Rapid Diagnostic Test, from three different sites...