A5042032473- Liver physiology and pathology
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Potato Plant Research
- Reproductive System and Pregnancy
- Electrospun Nanofibers in Biomedical Applications
- Drug Transport and Resistance Mechanisms
- Bone Tissue Engineering Materials
- Endometrial and Cervical Cancer Treatments
- Pain Mechanisms and Treatments
- Lung Cancer Research Studies
- Peptidase Inhibition and Analysis
- Phytochemical Studies and Bioactivities
- Liver Disease Diagnosis and Treatment
- Silk-based biomaterials and applications
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Plant Pathogenic Bacteria Studies
- Pharmacological Effects of Natural Compounds
- Hepatitis B Virus Studies
- Tendon Structure and Treatment
- ATP Synthase and ATPases Research
- PI3K/AKT/mTOR signaling in cancer
- Advances in Cucurbitaceae Research
- Cancer-related Molecular Pathways
E-Da Hospital
2016-2024
I-Shou University
2007-2024
Bridge University
2021
University of Tasmania
2021
Kaohsiung Chang Gung Memorial Hospital
2016
Kaohsiung Medical University
2004-2007
Chia Nan University of Pharmacy and Science
2007
National Cheng Kung University
2007
Saikosaponin d (SSd) is one of the main active triterpene saponins in Bupleurum falcatum. It has a steroid-like structure, and reported to have pharmacological activities, including liver protection rat, cell cycle arrest apoptosis induction several cancer lines. However, biological functions molecular mechanisms mammalian cells under SSd treatment are still unclear.The cytotoxicity hepatic stellate (HSCs) upon were discovered by MTT assay, colony formation assay flow cytometry. The collage...
Saikosaponin a (SSa) is one of the main active components Bupleurum falcatum. It commonly used to treat liver injury and fibrosis in traditional Chinese medicine. Our previous study showed that SSa induces apoptosis inhibits proliferation rat hepatic stellate cell (HSC) line HSC-T6. The aim present was elucidate mechanism SSa-mediated apoptosis. Rat HSC HSC-T6 human LX-2 were this study. triggered death mainly by apoptosis, as indicated typical morphological changes, sub-G1 phase cycle...
We have previously demonstrated that solamargine (SM), the major steroidal glycoalkaloid extracted from Chinese herb Solanum plants, reveals down-regulation of HER2 and up-regulation Fas tumor necrosis factor receptor (TNFR) expressions, triggers mitochondria-mediated cell apoptosis pathway, sensitizes human nonsmall lung cancer (NSCLC) H441 A549 adenocarcinoma cells to chemotherapy. The present study shows SM enhances expression in NSCLC large carcinoma H661 small (SCLC) H69 may increase...
Nonsmall-cell lung cancer (NSCLC) is not generally a chemosensitive tumor, and the mechanism of resistance to relevant anticancer drugs has been fully elucidated. Solamargine (SM), major steroidal glycoalkaloids extracted from Chinese herb Solanum, inhibits growth human tumor cells. We have previously demonstrated that SM regulates necrosis factor receptors (TNFRs)- mitochondria-mediated pathways sensitizes NSCLC cells initiate apoptosis. Interestingly, this investigation reveals...
Mutations on epidermal growth factor receptor (EGFR) of adenocarcinomas lung have been found to be associated with increased sensitivity EGFR tyrosine kinase inhibitors and K-ras mutations may correlate primary resistance. We aimed explore the discordant mutation statuses between tumors matched brain metastases in lung. used a sensitive Scorpion ARMS method analyze mutation, Sanger sequencing followed by allele-specific real-time polymerase chain reaction mutation. Forty-nine paired tissues...
The dose-dependent effects of adipose-derived mesenchymal stem cell-conditioned medium (ADSC-CM) were compared with those shockwave (SW) therapy in the treatment early osteoarthritis (OA). Anterior cruciate ligament transaction (ACLT) medial meniscectomy (MMx) was performed rats divided into sham, OA, SW, CM1 (intra-articular injection 100 μL ADSC-CM knee OA), and CM2 200 ADSC-CM) groups. Cartilage grading, grading synovium changes, specific molecular analysis by immunohistochemistry...
Abstract Saikosaponin d (SSd), a primary active component of the Chinese herb Bupleurum falcatum, has antitumor and antiliver fibrosis effects. However, toxicity SSd at high doses can induce conditions such as metabolic disorders hemolysis in vivo, thus hampering its clinical use. The present study investigated toxicity-reducing effects liposome encapsulation pure therapeutic action SSd-loaded liposomes (Lipo-SSd) liver vitro vivo. Lipo-SSd (diameter, 31.7 ± 7.8 nm) was prepared an...
Abstract Cirrhosis refers to irreversible liver damage where healthy tissue is replaced by scar tissue, resulting in impaired function. There no cure and current treatments only prevent further damage; thus, novel therapeutic options are urgently needed. Here, we report a new approach that enables the formation of self-assembled 3D spheroids adipose-derived stem cells (ADSCs) murine hepatocytes (AML12) via reconstituted collagen fibers. Compared with formed commercially available EZSHERE...
Introduction Endometriosis is defined as the growth of endometrial glands and stromal cells in a heterotopic location with immune dysregulation. It usually leads to chronic pelvic pain subfertility. Although various treatments are available, recurrence rate remains high. Adipose tissue an abundant source multipotent mesenchymal adipose-derived stem (ADSCs). ADSCs display effects on not only regeneration, but also regulation. Thus, current study aims test endometriosis. Methods isolated from...
Endometriosis, defined as the growth of endometrial glands and stromal cells in a heterotopic location under cyclic influence ovarian hormones, is common gynecological disorder manifested by chronic pelvic pain infertility. In traditional Chinese medicine, endometriosis characterized stagnation vital energy (qi) blood stasis. Guizhi Fuling Wan (GFW) was first described canonical medicine to treat disorders associated with qi stasis, including endometriosis. Therefore, current study aimed...
Hericium erinaceus is well known for the neurotrophic effect it confers by promoting nerve growth factor biosynthesis. We discovered a novel bioactivity of H. in its ability to suppress adenosine triphosphate (ATP)-induced calcium signaling neuronal PC12 cells. ATP, primarily as neurotransmitter, also acts on purinoceptors (P2 purinergic receptor [P2R]) generate cellular and secretion that mediate P2R physiological manifestations, including pain. Chronic pain reduces quality life. However,...
Neuropathic pain is a serious clinical problem that difficult to treat. Purinoceptors (P2Rs) transduce perception from the peripheral central nervous system and play an important role in transmission of neuropathic signals. We previously found crude extracts Hericium erinaceus mycelium (HE-CE) inhibited P2R-mediated signaling cells reduced heat-induced mice. The present study explored effects HE-CE on pain. used adenosine triphosphate (ATP) as P2R agonist generate Ca2+ neuronal damage cell...
Esophageal squamous cell carcinoma (ESCC), one of the most lethal cancers, has become a global health issue. Stearoyl-coA desaturase 1 (SCD1) been demonstrated to play crucial role in human cancers. However, pan-cancer analysis revealed little evidence date. In current study, we systematically inspected expression patterns and potential clinical outcomes SCD1 multiple was dysregulated several types its aberrant acted as diagnostic biomarker, indicating that may tumorigenesis. We used ESCC an...
Abstract Carnosic acid (CA), one of the purified components rosemary, has shown anti-inflammatory, chemo-preventive, and anticancer activities. In this study, we treated melanoma cell line B16F10 with CA detected effects. After 24-hour treatment, decreased proliferation rate, wound healing activity, inhibited colony formation B16F10. Additionally, induced G0/G1 cycle arrest, up-regulated p21 expression, down-regulate cyclin E, D CDK4 expression. Carmustine (BCNU) Lomustine (CCNU) are used in...
Abstract Cirrhosis refers to irreversible liver damage where healthy tissue is replaced by scar which impairs function. There no cure and current treatments only prevent further damage; thus novel therapeutic options are urgently needed. Here, we report a new approach that enables the formation of self-assembled 3D spheroids adipose-derived stem cells (ADSCs) murine hepatocytes (AML12) via reconstituted collagen fibers. Compared with formed in commercially available EZSHERE dish, fiber-based...