A5100683867- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Poxvirus research and outbreaks
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Chronic Lymphocytic Leukemia Research
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Aquaculture Nutrition and Growth
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Soil Carbon and Nitrogen Dynamics
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Climate change and permafrost
- Lymphoma Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
Stanford University
2008-2025
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences
2022-2025
Guiyang Medical University
2022-2025
Northwest Institute of Eco-Environment and Resources
2024
Chinese Academy of Sciences
2024
Ministry of Education
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Shaanxi Normal University
2023
iRepertoire (United States)
2012-2022
First Affiliated Hospital Zhejiang University
2020
Developing T cells face a series of cell fate choices in the thymus and periphery. The role individual receptor (TCR) determining decisions remains unresolved. stochastic/selection model postulates that initial is independent TCR specificity, with survival dependent on additional TCR/coreceptor “rescue” signals. “instructive” holds initiated by interaction cognate peptide-MHC complex. are then segregated basis specificity aid critical coreceptors signal modulators [Chan S, Correia-Neves M,...
The diversity of virus populations within single infected hosts presents a major difficulty for the natural immune response as well vaccine design and antiviral drug therapy. Recently developed pyrophosphate based sequencing technologies (pyrosequencing) can be used quantifying this by ultra-deep samples. We present computational methods analysis such sequence data apply these techniques to pyrosequencing obtained from HIV patients harboring resistant strains. Our main result is estimation...
Poxviruses are highly successful pathogens, known to infect a variety of hosts. The family Poxviridae includes Variola virus, the causative agent smallpox, which has been eradicated as public health threat but could potentially reemerge bioterrorist threat. risk scenario other animal poxviruses and genetically engineered manipulations poxviruses. Studies orthologous gene sets have established evolutionary relationships members within family. It is not clear, however, how variations between...
Human leukocyte antigen (HLA) genes are the most polymorphic in human genome. They play a pivotal role immune response and have been implicated numerous pathologies, especially autoimmunity infectious diseases. Despite their importance, however, they rarely characterized comprehensively because of prohibitive cost standard technologies technical challenges accurately discriminating between these highly related many allelles. Here we demonstrate high-resolution, cost-effective methodology to...
The primary cause of poor outcome following allogeneic hematopoietic cell transplantation (HCT) for chronic lymphocytic leukemia (CLL) is disease recurrence. Detection increasing minimal residual (MRD) HCT may permit early intervention to prevent clinical relapse; however, MRD quantification remains an uncommon diagnostic test because logistical and financial barriers widespread use. Here we describe a method quantifying CLL using widely available consensus primers amplification all Ig heavy...
Processes that define immunoglobulin repertoires are commonly presumed to be the same for all murine B cells. However, studies here couple high-dimensional FACS sorting with large-scale quantitative IgH deep-sequencing demonstrate B-1a repertoire differs dramatically from follicular and marginal zone cells is defined by distinct mechanisms. We track their early appearance in neonatal spleen long-term residence adult peritoneum spleen. show de novo rearrangement mainly occurs during first few...
Abstract CTLA-4 is an important regulator of T-cell function. Here, we report that expression this immune-regulator in mouse B-1a cells has a critical function maintaining self-tolerance by regulating these early-developing B express repertoire enriched for auto-reactivity. Selective deletion from results mice spontaneously develop autoantibodies, T follicular helper (Tfh) and germinal centers (GCs) the spleen, autoimmune pathology later life. This impaired immune homeostasis cell...
Disruption of colonic homeostasis caused by aberrant M1/M2 macrophage polarization contributes to the development inflammatory bowel disease (IBD). Lycium barbarum polysaccharide (LBP) is primary active constituent traditional Chinese herbal L., which has been widely demonstrated have important functions in regulating immune activity and anti-inflammatory. Thus, LBP may protect against IBD. To test this hypothesis, DSS-induced colitis model was established mice, then mice were treated with...
Abstract Analyzing antigen-specific T cell responses at scale has been challenging. Here, we analyze three types of receptor (TCR) repertoire data (antigen-specific TCRs, TCR-repertoire, and single-cell RNA + TCRαβ-sequencing data) from 515 patients with primary or metastatic melanoma compare it to 783 healthy controls. Although melanoma-associated antigen (MAA) -specific TCRs are restricted individuals, they share sequence similarities that allow us build classifiers for predicting anti-MAA...
Abstract The changes and influencing factors of soil inorganic carbon (SIC) organic (SOC) on precipitation gradients are crucial for predicting evaluating storage at the regional scale. However, people’s understanding distribution characteristics SOC SIC reserves is insufficient, main environmental variables that affect also not well understood. Therefore, this study focuses Alxa region selects five regions covered by three typical desert vegetation types, Zygophyllum xanthoxylon (ZX),...
Plaque psoriasis is an inflammatory skin disorder affecting nearly 2% of the world population. Despite recent advances in treatment, there still a need for more effective therapies. The ETS transcription factor FLI1 plays critical roles hematopoiesis, angiogenesis, immunity, and cancer. Emerging evidence suggests that intricately involved processes underlying pathogenesis. RNAseq bioinformatic analysis were used to identify correlation between levels expression genes associated with...
Poxviruses engage in a complex and intricate dialogue with host cells as part of their strategy for replication. However, relatively little molecular detail is available which to understand the mechanisms behind this dialogue.
Specific changes in immune repertoires at genetic level responding to the lethal H7N9 virus are still poorly understood. We performed deep sequencing on T and B cells from patients recently infected with explore correlation between clinical outcomes repertoire alterations. cell display highly dynamic yet distinct clonotype During infection, beta chain continues contract while diversity of immunoglobulin heavy recovers. Patient recovery is correlated different ways - higher lower found...
Abstract Background FLI1 is an oncogenic transcription factor that promotes diverse malignancies through mechanisms are not fully understood. Herein, shown to regulate the expression of Ubiquitin Associated and SH3 Domain Containing A/B ( UBASH3A/B ) genes. UBASH3B UBASH3A found act as oncogene tumor suppressor, respectively, their combined effect determines erythroleukemia progression downstream FLI1. Methods Promoter analysis with luciferase assays chromatin immunoprecipitation (ChIP) were...
Recent evidence indicates the presence of macrophage subpopulations that express TCRαβ in chronic inflammatory diseases such as tuberculosis and atherosclerosis tumor microenvironment. Here, we demonstrate a second subpopulation macrophages expresses rearranged heavy light chain immunoglobulins. We identify immunoglobulin expression human murine monocytes, ex vivo differentiated from microenvironment five randomly selected distinct entities. The chains are expressed small subfraction (~3–5%)...
Abstract TRIM27 (tripartite motif‐containing 27) is a member of the TRIM motif) protein family and participates in variety biological processes. Some research has reported that was highly expressed certain kinds carcinoma cells tissues played an important role proliferation cells. However, whether takes part progression lupus nephritis (LN) especially remains unclear. Our study revealed overexpression observed kidneys patients with LN, mice mesangial exposed to LN plasma which correlated ECM...
To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed content of representative members and determined whether individual genes present each genome were intact, truncated, or fragmented. When nonintact identified, early stop mutations (ESMs) leading truncation fragmentation analyzed. Of all ESMs these poxvirus genomes, over 65% co-localized with microsatellites—simple sequence nucleotide repeats. On average, microsatellites comprise 24% genomes....