A5024565466- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- Adrenal Hormones and Disorders
- Vascular Malformations Diagnosis and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Effects and risks of endocrine disrupting chemicals
- Stress Responses and Cortisol
- Sepsis Diagnosis and Treatment
- Liver Disease and Transplantation
- Reproductive Physiology in Livestock
- Airway Management and Intubation Techniques
- Renal and related cancers
- Regulation of Appetite and Obesity
- Infant Nutrition and Health
- Organ Transplantation Techniques and Outcomes
- Cardiac Arrest and Resuscitation
- Testicular diseases and treatments
- Growth Hormone and Insulin-like Growth Factors
- Metabolism and Genetic Disorders
- Reproductive Health and Technologies
Bicêtre Hospital
2015-2024
Assistance Publique – Hôpitaux de Paris
2012-2023
Université Paris-Saclay
2011-2023
Institut Arnault Tzanck
2023
Université Claude Bernard Lyon 1
2004-2022
Université de Lorraine
2020-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2014-2022
VetAgro Sup
2021-2022
Institut Cellule Souche et Cerveau
2022
Institut de Chimie des Substances Naturelles
2017-2018
Abstract Background Point-of-care ultrasound (POCUS) is nowadays an essential tool in critical care. Its role seems more important neonates and children where other monitoring techniques may be unavailable. POCUS Working Group of the European Society Paediatric Neonatal Intensive Care (ESPNIC) aimed to provide evidence-based clinical guidelines for use critically ill children. Methods Creation international Euro-American panel paediatric neonatal intensivists expert systematic review...
Cryopreservation induces many changes in sperm cells, including membrane disorders and cell death. We tested the hypothesis that apoptosis, a form of programmed death, can contribute to fatal effect cryopreservation on cells. A multiparametric study apoptosis bovine is proposed, using flow cytometry, mitochondrial potential (ΔΨm), caspase activation, permeability, nucleus condensation, DNA fragmentation, phosphatidylserine (PS) externalization. The relevance each test was first validated...
Objectives: To evaluate the feasibility and outcome of noninvasive positive pressure ventilation (NPPV) in daily clinical practice. Design: Observational retrospective cohort study. Setting: Pediatric intensive care unit a university hospital. Patients: Patients treated by NPPV, regardless indication, during five consecutive years (2000–2004). Measurements Results: A total 114 patients were included, 83 (77%) successfully NPPV without intubation (NPPV success group). The rate was...
The aim of this study was to review our experience in orthotopic liver transplantation (OLT) for biliary atresia (BA) children and analyze the survival prognostic factors, long-term outcome. We reviewed 332 OLTs performed 280 between years 1986 2000. Univariate multivariate analysis were on patient graft survivals according recipients' donors' characteristics as well intraoperative data. outcome among 80 living at 10 after OLT studied growth, immunosuppressive therapy, renal functions. Liver...
BACKGROUND: In cases of male infertility, routine analysis for sperm characteristics is a poor predictive factor the segmentation rate and embryo development in assisted reproductive technologies. It assumed that epigenetic factors could have an influence on embryo's quality. The aim this work was to determine relationship between DNA methylation level fertilization pregnancy rates according reproduction technique performed. METHODS: A prospective study undertaken. Ejaculates were obtained...
Until now, complete ex vivo spermatogenesis has been reported only in the mouse. In this species, duration of is 35 days, whereas it 54 days rat and 74 humans. We performed long-term (until 60 days) cultures fresh or frozen human seminiferous tubule segments a bioreactor made hollow cylinder chitosan hydrogel. Testicular tissues were obtained from 8- 20-day-old male rats adult subjects who had undergone hormone treatments leading to nearly regression their before bilateral orchiectomy for...
Multisystem inflammatory syndrome in children (MIS-C) is the most severe clinical entity associated with pediatric SARS-CoV-2 infection a putative role of spike protein into immune system activation. Whether COVID-19 mRNA vaccine can induce this complication unknown. We aimed to assess risk hyper-inflammatory following children.
In the present study we developed a model of diet-induced obesity (DIO) in male C57 BL/6J mice using an 8-wk high fat diet. This should better reflect physiology majority human obese patients than mouse genetic models with defects leptin or signaling. At end diet, DIO displayed increased weight (20%) and higher leptin, insulin, glucose, corticosterone plasma levels compared fed standard diet during same period. Moreover, they became resistant to central effect peripheral administration...
Levels of insulin-like growth factor-I (IGF-I), IGF-II, and IGF-binding proteins (IGFBPs) were studied in ovine follicular fluid serum with a view to determining their respective endocrine and/or paracrine roles ovarian folliculogenesis. Ovarian follicles Ile-de-France ewes dissected measured individually, collected. Follicles classified according size degree atresia, assessed on the basis microscopical examination smears granulosa cells. Follicular samples assayed for IGF-I IGF-II. Free...
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
Background. About 10% of pediatric patients with invasive pneumococcal disease (IPD) die from the disease. Some primary immunodeficiencies (PIDs) are known to confer predisposition IPD. However, a systematic search for these PIDs has never been carried out in children presenting Methods. We prospectively identified cases IPD requiring hospitalization between 2005 and 2011 28 wards throughout France. was defined as positive culture, polymerase chain reaction result, and/or soluble antigen...
Background The objective was to determine whether high-flow nasal cannula (HFNC), a promising respiratory support in infant bronchiolitis, could reduce the proportion of treatment failure requiring escalation care. Methods In this randomised controlled trial, we assigned infants aged <6 months who had moderate bronchiolitis receive either HFNC at 3 L·kg −1 ·min or standard oxygen therapy. Crossover not allowed. primary outcome patients care (mostly noninvasive ventilation) within 7 days...
Transforming growth factors β1 and β2 (TGFβs) have recently been detected by immunohistochemistry in the fetal neonatal rat testis, aim of present study was to determine whether these can act as local regulators control number gonocytes. Testes were kept organ culture, TGFβ1 found dose-dependent inhibitory effect on gonocytes testes explanted day 13.5. Either or at 10 ng/ml reduced half after 2 days culture. TGFβs did not decrease BrdU labeling index Sertoli cells, whereas significantly...
We investigated the effect of retinoids on development Sertoli, germ, and Leydig cells using 3-day culture testes from fetuses 14.5 18.5 days post-conception (dpc) neonates 3 postpartum (dpp). Addition 10−6 M 3.10−8 retinoic acid (RA) caused a dose-dependent disruption seminiferous cords in 14.5-day-old fetal testes, without any change 5-bromo-2′-deoxyuridine (BrdU) labeling index Sertoli cells. RA no disorganization older but it did cause hyperplasia 3-dpp testes. Fragmentation cell DNA was...
Translocation of phosphatidylserine (PS) from the inner to outer leaflet plasma membrane is a modification lipid architecture occurring in sperm. This one earliest signs apoptosis that can be monitored by calcium-dependent binding annexin V.Flow cytometric analysis V was performed. Calcium ionophore A23187 led significant increase proportion living sperm with PS exposure: 7.3 3.2% cells untreated ejaculate versus 47.5 5.6% after 1 h incubation A23187. Conversely, diminution mitochondrial...
Background Bisphenol A (BPA) is one of the most widespread chemicals in world and suspected being responsible for male reproductive impairments. Nevertheless, its molecular mode action on spermatogenesis unclear. This work combines physiology toxicogenomics to identify mechanisms by which BPA affects timing meiosis induces germ-cell abnormalities. Methods We used a rat seminiferous tubule culture model mimicking vivo adult situation. (1 nM 10 nM) was added medium. Transcriptomic meiotic...