A5100374469- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Immunotoxicology and immune responses
- Hematopoietic Stem Cell Transplantation
- NF-κB Signaling Pathways
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Carcinogens and Genotoxicity Assessment
- Chronic Lymphocytic Leukemia Research
- Virus-based gene therapy research
- Platelet Disorders and Treatments
- HIV Research and Treatment
- Systemic Lupus Erythematosus Research
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Toxin Mechanisms and Immunotoxins
- Neurogenesis and neuroplasticity mechanisms
- Cholesterol and Lipid Metabolism
- Chronic Myeloid Leukemia Treatments
Jinan University
2012-2024
Southern University of Science and Technology
2019-2024
Nankai University
2024
Inner Mongolia University
2024
Jinzhou Medical University
2010-2023
Soochow University
2023
The University of Texas Southwestern Medical Center
2022
Ezhou Central Hospital
2019
Chongqing Three Gorges Central Hospital
2017
Sichuan University
2011-2017
Gastrointestinal (GI) cancer is characterized by its aggressiveness and tendency to metastasize at early stage. Epithelial-mesenchymal transition (EMT), commonly known as the preparing step of metastasis, may account for aggressive phenotype GI cells. The process EMT finely orchestrated multiple layers regulators. Signal transducer activator transcription 3 (STAT3) a factor constitutively activated in diverse malignancies. Recent studies have suggested an involvement STAT3 EMT. In this...
The interaction between Human leukocyte antigens (HLA) and T cell receptor (TCR) is essential for adaptive immune recognition. While it known that one TCR can map to multiple HLA alleles, the extent of this cross-reactivity remains poorly understood. Here, we introduce THNet, a TCR-based similarity inference method, performed comprehensive analysis HLA-TCR cross-reactivity. This method built upon clustering over 47 million TCRs identify 9 significant pairs. We created networks both class I...
Abstract Toll-IL-1R domain-containing adaptor-inducing IFN-β (TRIF) is an adaptor molecule that mediates a distinct TLR signaling pathway. Roles of TRIF in the host defense have been primarily associated with virus infections owing to induction IFN-αβ. In this study, we investigated role Pseudomonas aeruginosa infection. vitro, TRIF-deficient mouse alveolar and peritoneal macrophages showed complete inhibition RANTES (CCL5) production, severely impaired TNF KC (CXCL1) reduced NF-κB...
Rhabdomyosarcoma (RMS) is a common pediatric soft-tissue tumor. In this study, we evaluated the efficacy and selectivity of drozitumab, death receptor DR5-targeted therapeutic antibody, in RMS preclinical models.A panel 11 cell lines was used for vitro studies. The molecular marker predictive response to drozitumab interrogated. Selected were injected into gastrocnemius muscle mice vivo assessment potency drozitumab.We report that DR5, but not DR4, persisted at high levels on surface all...
Abstract Although evolutionarily just as ancient IgM, it has been thought for many years that IgD is not present in birds. Based on the recently sequenced genomes of 48 bird species well high-throughput transcriptome sequencing immune-related tissues, we demonstrate this work ostrich (Struthio camelus) possesses a functional δ gene encodes membrane-bound H chain with seven CH domains. Furthermore, sequences were clearly identified other species, demonstrating widely distributed among birds...
T-cell dysregulation is a major event involved in immune thrombocytopenic purpura (ITP). Increasing data have indicated that abnormal expression of immunosuppressive receptors, such as programmed death (PD) 1 and cytotoxic T lymphocyte antigen-4 (CTLA-4), may be related to autoimmune disease pathogenesis.We analyzed the levels PD-1, its ligand PD-L1, CTLA-4 peripheral blood mononuclear cells from 18 patients with chronic ITP by real-time polymerase chain reaction, samples 20 healthy...
Aplastic anemia (AA) is characterized by pancytopenia and bone marrow hypoplasia, which results from immune-mediated hematopoiesis suppression. Understanding the pathophysiology of immune system, particularly T cells immunity, has led to improved AA treatment over past decades. However, primary secondary failure after immunosuppressive therapy frequent. Thus, knowledge mechanisms leading crucial fundamentally understand disease. To elucidate cell receptor (TCR) signal transduction features...
Abstract Background Proliferation and migration of vascular smooth muscle cells (VSMCs) play a key role in neointimal formation which leads to restenosis vein graft venous bypass. STAT-3 is transcription factor associated with cell proliferation. We hypothesized that silencing by siRNA will inhibit proliferation VSMCs attenuate intimal thickening. Methods Rat were isolated cultured vitro applying tissue piece inoculation methods. transfected STAT 3 using lipofectamine 2000. In VSMC was...
// Haojun Xiong 1 , Yan Zhang Shan Chen Zhenhong Ni Jintao He 2 Xinzhe Li Bo Kai Zhao Fan Yang Yijun Zeng Bingbo 3 and Fengtian Department of Biochemistry Molecular Biology, College Basic Medical Sciences, Third Military University, Chongqing 400038, China Battalion 17 Students, Preventive Medicine, Laboratory Animal Center, Correspondence to: He, email: hefengtian06@aliyun.com Chen, chenbb81@126.com Keywords: HCC, LXR, SOCS3 Received: April 28, 2017 Accepted: June 10, Published: July 18,...
A previous study has demonstrated a significantly increased CD3ζ gene expression level in aplastic anemia (AA). However, the mechanism underlying upregulated mRNA and that of T cell activation signaling molecules AA patients remains unclear. Thus, we investigated levels CD3ζ, CD28, CTLA-4, Cbl-b genes, SNP rs231775 CTLA-4 gene, distribution 3′-UTR splice variant patients. variants were identified peripheral blood mononuclear cells (PBMCs) from 48 healthy individuals 67 with [37 cases severe...
T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, further characterize the immune status, T cell proliferative history was analyzed CD4+ CD8+ cells from chronic myeloid leukemia (CML) patients. Quantitative analysis δRec-ψJα signal joint receptor excision circles (sjTRECs) performed PBMCs, CD3+, CD8+T by real-time PCR, 23 TRBV-D1 sjTRECs semi-nested PCR. Forty eight CML cases phase (CML-CP)...
In order to elucidate the feature of T‐cell receptor (TCR) signal transduction in T‐cells from acute myeloid leukemia (AML), expression levels CD3gamma, delta, epsilon and zeta chain genes CD3+ T cells were analyzed using real‐time PCR. sorted peripheral blood 10 AML patients healthy donors used study. Significantly lower all four epsilon, found samples. The pattern CD3 chains was epsilon>gamma>delta>zeta samples, which different control group. conclusion, results provide a global gene...
To elucidate the characteristics of T-cell receptor (TCR) signal transduction in T-cells from acute myeloid leukemia (AML), mucosa-associated-lymphoid-tissue lymphoma-translocation gene 1 (MALT1), A20, NF-κB and MALT1-V1 expression levels CD3+ T cells sorted peripheral blood patients with AML were analyzed by real-time PCR. A significantly lower MALT1 A20 level was found compared healthy controls (p = 0.045, p < 0.0001); however, (variant 1) higher group than control 0.006), increased group....
Live attenuated SIV induces potent protection against superinfection with virulent virus; however the mechanism of this vaccine effect is poorly understood. Such knowledge important for development clinically acceptable modalities HIV.Using a novel, doxycycline dependent, replication-competent live-attenuated SIVmac239Δnef (SIV-rtTAΔnef), we show that under replication-permissive conditions SIV-rtTAΔnef fully viable. Twelve rhesus macaques were infected peak plasma vRNA on average two log10...
Cell-mediated immunity is often suppressed in patients with hematological malignancies. Recently, we found that low T cell receptor (TCR)-CD3 signaling was related to abnormal expression of the negative regulator nuclear factor kappa B (NF-κB) A20 acute myeloid leukemia. To investigate characteristics immunodeficiency lymphomas, analyzed features and its upstream regulating mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) genes downstream NF-κB different subtypes,...
T cell immunity plays a central role in the body's defense system, including maintaining homeostasis and preventing tumorigenesis viral infection. Immune system functions degenerate with age, leading to immune senescence. Physiologically, senescence is characterized by decrease receptor diversity, naive deficiency, alterations immune-related miRNAs. However, little known about characteristics of immunosenescence Chinese individuals.A significant miR-17, miR-92a, miR-181a levels PBMCs was...
Single base mutations on a dsDNA substrate with an AP site incorporated could be easily detected through one-pot reaction of enzymes.
In leukemia patients, T-cell function has been suppressed with the disease progress. Patients chronic lymphocytic (CLL) are all to a degree immunodeficient. order elucidate feature of receptor signal transduction in CLL, expression levels CD3γ, δ, ɛ, and ζ chain, FcɛRIγ, Zap-70 genes peripheral blood mononuclear cells (PBMCs) were analyzed. Real-time polymerase chain reaction SYBR Green technique was used for detecting gene level PBMCs from 13 patients healthy individuals, 10 B-cell acute...