A5100416006- Cancer-related molecular mechanisms research
- Animal Behavior and Welfare Studies
- Angiogenesis and VEGF in Cancer
- Genetic and phenotypic traits in livestock
- Circular RNAs in diseases
- Beetle Biology and Toxicology Studies
- Cancer, Hypoxia, and Metabolism
- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Venomous Animal Envenomation and Studies
- MicroRNA in disease regulation
- Plant nutrient uptake and metabolism
- Renal and related cancers
- Genetic Mapping and Diversity in Plants and Animals
- Pluripotent Stem Cells Research
- Nuts composition and effects
- Reproductive Biology and Fertility
- Cancer, Lipids, and Metabolism
- Pharmacological Effects of Natural Compounds
- Medicinal Plant Pharmacodynamics Research
- Cancer Research and Treatments
- Cytomegalovirus and herpesvirus research
- Coenzyme Q10 studies and effects
- Quinazolinone synthesis and applications
Digital China Health (China)
2024
National Clinical Research
2024
Harbin Medical University
2024
Peking University
2024
Central Hospital of Zibo
2021-2023
University of Benin
2023
Shanghai University of Traditional Chinese Medicine
2023
Hebei Agricultural University
2005-2020
Guangdong Medical College
2016-2020
Shandong First Medical University
2008-2016
Vascular endothelial cell growth factor (VEGF), an cell-specific mitogen that plays important role in angiogenesis, promotes the tyrosine phosphorylation of at least 11 proteins bovine aortic cells (BAEC). Proteins immunoprecipitated from lysates control- and VEGF-stimulated BAEC with antisera to phospholipase C- γ (PLC- γ) were fractionated by SDS-polyacrylamide gel electrophoresis transferred Immobilon-P. Evaluation Western blots phosphotyrosine demonstrated PLC- two (100 85 kDa) associate...
Glucocorticoids exert a well-known catabolic protein action on skeletal muscle. The mechanistic target of rapamycin (mTOR) signaling pathway acts as central regulator metabolism. Whether glucocorticoids regulate synthesis through the mTOR in muscle chickens remains unknown. This study was performed to characterize effect development chickens, and cultured embryonic myoblasts. Male 29-d-old were given dexamethasone injection (2 mg/kg) twice per day for 4 d (n = 16). Chicken myoblasts exposed...
Single nucleotide variants (SNVs), as important components of genetic variation, affect gene expression, function and phenotype. Mining summarizing the spatial distribution SNVs in diseased normal tissues for a better understanding their characteristics potential roles cell-lineage determination, aging, or disease occurrence is significant. Herein, we have developed comprehensive mutation resource stSNV (http://bio-bigdata.hrbmu.edu.cn/stSNV/index.jsp), which provides an atlas major human...
Abstract Background Improving sow fertility is extremely important as it can lead to increased reproductive efficiency and thus profitability for swine producers. There are considerable differences in rates among individual animals, but the underlying molecular mechanisms remain unclear. In this study, by using different types of RNA libraries, we investigated complete transcriptome ovarian tissue during luteal (L) follicular (F) phases estrous cycle Large White pigs with high (H) low...
The aim of this paper was to determine whether the genetic background tetraploid embryos contributed survival mice derived from embryonic stem (ES) cells by embryo complementation. Twenty-five newborns were produced aggregation hybrid ES and with different backgrounds. These entirely judged microsatellite DNA (A specific sequence bases or nucleotides that contains mono, di, tri tetra repeats) coat colour phenotype germline transmission. Fifteen survived adulthood while seven died respiratory...
The aim of the present study was to investigate role microRNAs (miRNAs/miRs) in anti‑fibrotic effect astaxanthin (AST), using human hepatic stellate cell (HSC) line LX‑2 as research model. cells were treated with various concentrations AST (10, 20 and 40 µM) for 24 or 48 h. miR‑29b selected based on existing literature, its targeting gene B lymphoma (Bcl)‑2 predicted by TargetScan miRanda databases further analysis. Interactions between Bcl‑2 evaluated reverse transcription‑quantitative...
In this study, the ovarian tissues of Large White pigs were mined for novel circular RNAs (circRNAs), following which, their molecular characteristics and potential mechanisms fertility regulation examined. RNA sequencing was used transcriptome analysis follicles corpora lutea in sows with high (H) low (L) during follicular (F) luteal phases estrous cycle. total, 21,386 circRNA derived from 4535 host genes identified. Differentially expressed circRNAs detected LH vs. LL (1079) FH FL (1077)...
Gallbladder carcinoma (GC) is an extremely malignant gastrointestinal tumor, but relevant mechanisms are still under investigation. MicroRNA (miR) differentially expressed in a variety of tumors. Here we explored miR-204 patients with GC and related mechanisms. A GSE104165 chip was downloaded from the gene expression omnibus (GEO) for analysis. The qRT-PCR assay used quantifying Notch2 serum tissues patients, were followed up 3 years to analyze independent factors prognosis. CCK8, transwell,...
To study the effects of polypeptide extract from scorpion venom (PESV) on immune escape Lewis lung carcinomas (LLC) and its mechanism.Forty C57BL/6J mice were inoculated with LLC cells suspension (1 x 10(7) cells/ mL) in right armpit subcutaneously. The tumor-bearing randomly divided into two groups: control group PESV group. was intragastrically subjected to experimental for 18 days. tumor volume inhibitory rate determined. expression levels VEGF,TGF-beta1 IL-10 microenvironment determined...
To investigate the mechanisms for inhibition effects of PESV on proliferation non-small cell lung cancer line A549.MTT was used to observe growth and A549 at different concentrations PESV. Flow cytometry (FCM) applied analyze cycle distribution. Immunocytochemistry western blot assay recruited detect expression VEGF, HIF-1alpha, PTEN after intervention PESV.A549 cells may be arrested mainly in G0/G1 phase significantly inhibited (P < 0.01) a certain range concentration. can reduce...
Abstract Although the epigenetic mechanisms underlying bone formation are recognized, their specific roles and regulatory pathways remain largely unexplored. In this study, we unveil PRMT7 as a novel modulator of MSCs’ osteogenic commitment. The conditional knockout Prmt7 in mice reveals significantly impaired osteogenesis regeneration exclusively females, affecting both long bones craniofacial structures, with no discernible impact males. Our findings demonstrate that orchestrates...