A5037255370- Neuroinflammation and Neurodegeneration Mechanisms
- CNS Lymphoma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Solid-state spectroscopy and crystallography
- Electron Spin Resonance Studies
- Muon and positron interactions and applications
- Immune cells in cancer
- Photonic and Optical Devices
- Peripheral Neuropathies and Disorders
- Cytomegalovirus and herpesvirus research
- Advanced NMR Techniques and Applications
- Ocular Diseases and Behçet’s Syndrome
- Luminescence Properties of Advanced Materials
- Systemic Lupus Erythematosus Research
- interferon and immune responses
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Schizophrenia research and treatment
- Electrochemical Analysis and Applications
- Surface Roughness and Optical Measurements
- Fungal Infections and Studies
- Myasthenia Gravis and Thymoma
- Semiconductor materials and devices
University Hospital Münster
2018-2025
University of Münster
1981-2023
Schott (Germany)
1992-1996
University of Zurich
1986-1989
University of Houston
1985
Abstract Cerebrospinal fluid (CSF) protects the central nervous system (CNS) and analyzing CSF aids diagnosis of CNS diseases, but our understanding leukocytes remains superficial. Here, using single cell transcriptomics, we identify a specific location-associated composition transcriptome leukocytes. Multiple sclerosis (MS) – an autoimmune disease increases transcriptional diversity in blood, type including higher abundance cytotoxic phenotype T helper cells. An analytical approach, named...
B-cell maturation antigen (BCMA), expressed in plasmablasts and plasma cells, could serve as a promising therapeutic target for autoimmune diseases. We reported here chimeric receptor (CAR) T cells targeting BCMA two patients with highly relapsed refractory myasthenia gravis (one AChR-IgG, one MuSk-IgG). Both exhibited favorable safety profiles persistent clinical improvements over 18 months. Reconstitution of lineages sustained reduced pathogenic autoantibodies might underlie the efficacy....
Although glucocorticoids (GC) represent the most frequently used immunosuppressive drugs, their effects are still not well understood. In our previous studies, we have shown that treatment of monocytes with GC does cause a global suppression monocytic effector functions, but rather induces differentiation specific anti-inflammatory phenotype. The role peroxisome proliferator-activated receptor (PPAR)-γ has been extensively studied during recent years. However, relationship between and PPAR-γ...
Peripheral nerves contain axons and their enwrapping glia cells named Schwann (SCs) that are either myelinating (mySCs) or nonmyelinating (nmSCs). Our understanding of other in the peripheral nervous system (PNS) remains limited. Here, we provide an unbiased single cell transcriptomic characterization nondiseased rodent PNS. We identified independently confirmed markers previously underappreciated nmSCs nerve-associated fibroblasts. also found characterized two distinct populations...
Inflammation triggers secondary brain damage after stroke. The meninges and other CNS border compartments serve as invasion sites for leukocyte influx into the thus promoting tissue However, post-ischemic immune response of compared to parenchyma remains poorly characterized. Here, we deeply characterize tissue-resident leukocytes in discover that respond differently stroke depending on their site residence. We thereby a unique phenotype myeloid cells exclusive These stroke-associated...
Abstract Alemtuzumab is a monoclonal antibody that causes rapid depletion of CD52-expressing immune cells. It has proven to be highly efficacious in active relapsing–remitting multiple sclerosis; however, the high risk secondary autoimmune disorders greatly complicated its use. Thus, deeper insight into pathophysiology autoimmunity and potential biomarkers urgently needed. The most critical time points decision-making process for alemtuzumab therapy are before or at Month 12, where ability...
Chimeric antigen receptor (CAR) T cell immunotherapy for the treatment of neurological autoimmune diseases is promising, but CAR kinetics and immune alterations after are poorly understood. Here, we performed single-cell multi-omics sequencing paired cerebrospinal fluid (CSF) blood samples from patients with neuromyelitis optica spectrum disorder (NMOSD) treated anti-B maturation (BCMA) cells. Proliferating cytotoxic-like CD8
Myasthenia gravis (MG) is an autoimmune disease most frequently caused by autoantibodies (auto-Abs) against the acetylcholine receptor (AChR) located at neuromuscular junction. Thymic follicular hyperplasia present in of patients with early-onset AChR-Ab+ MG (EOMG), but its cellular and molecular drivers development remain poorly understood. We constructed a single cell-based transcriptional profile lymphoid cell types thymi from 11 immunotherapy-naïve EOMG. Multiplex histology ELISA were...
Abstract Background Primary central nervous system lymphoma (PCNSL) is a rare of the system, usually diffuse large B cell phenotype. Stereotactic biopsy followed by histopathology diagnostic standard. However, limited material available from CNS biopsies, thus impeding an in-depth characterization PCNSL. Methods We performed flow cytometry, single-cell RNA sequencing, and receptor sequencing PCNSL cells released material, blood, cerebrospinal fluid (CSF), spatial transcriptomics samples....
Objective Utilize immune cell profiles in the cerebrospinal fluid (CSF) to advance understanding and potentially support diagnosis of inflammatory neuropathies. Methods We analyzed CSF flow cytometry data patients with definite Guillain-Barré syndrome (GBS, n = 26) chronic demyelinating polyneuropathy (CIDP, 32) based on established diagnostic criteria comparison controls relapsing-remitting multiple sclerosis (RRMS, 49) idiopathic intracranial hypertension (IIH, 63). Results Flow revealed...
Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration leukocytes into the uveal tissues. associated with HLA haplotype B27 (HLA-B27) is common subtype uveitis and prototypical ocular immune-mediated disease. Local immune mechanisms driving human are poorly mainly due to limited available biomaterial subsequent technical limitations. Here, we provide first high-resolution characterization intraocular in HLA-B27-positive (n = 4) -negative 2)...
Microglia, the resident immune cells of central nervous system (CNS), are derived from yolk-sac macrophages that populate developing CNS during early embryonic development. Once established, microglia population is self-maintained throughout life by local proliferation. As a scalable source microglia-like (MGLs), we here present forward programming protocol for their generation human pluripotent stem (hPSCs). The transient overexpression PU.1 and C/EBPβ in hPSCs led to homogenous mature...
Abstract 5q-associated spinal muscular atrophy (SMA) is a motoneuron disease caused by mutations in the survival motor neuron 1 (SMN1) gene. Adaptive immunity may contribute to SMA as described other diseases, yet mechanisms remain elusive. Nusinersen, an antisense treatment, enhances SMN2 expression, benefiting patients. Here we have longitudinally investigated and nusinersen effects on local immune responses cerebrospinal fluid (CSF) - surrogate of central nervous system parenchyma....
Abstract Psychotic disorders are common and disabling mental conditions. The relative importance of immune-related mechanisms in psychotic remains subject debate. Here, we present a large-scale retrospective study blood cerebrospinal fluid (CSF) immune cell profiles psychosis spectrum patients. We performed basic CSF analysis multi-dimensional flow cytometry cells from 59 patients with primary (F20, F22, F23, F25) comparison to inflammatory (49 RRMS 16 NMDARE patients) non-inflammatory...
Significance The meninges protect the central nervous system but also host lymphocytes in neuroinflammation. In human multiple sclerosis, preferentially B cells accumulate meninges. By generating a compartment-specific transcriptional map of meningeal versus parenchymal leukocytes experimental neuroinflammation, we found follicular phenotype and corresponding helper-like Th17 cells. thus instructed site-specific local to proinflammatory autoreactive T We identified transcription factor Bcl6...
Abstract For the rate constant of addition tert ‐butyl radicals to acrylonitrile at T = 300 K in solution modulated ESR spectroscopy and muon spin rotation yield 10 6 M −1 s 2.4 × . The pivaloyl radical proceeds with Arrhenius parameters log A /M 7.7 E a 11.5 kJ/ mol. results are discussed terms polar effects reactions.
Distinguishing neurosarcoidosis (NS) from multiple sclerosis (MS) remains challenging and available parameters lack discriminatory power. Comprehensive flow cytometry data of blood CSF leukocytes patients with NS (n = 24), MS 49) idiopathic intracranial hypertension (IIH, n 52) were analyzed by machine learning algorithms. featured a specific immune cell pattern increased activated CD4+ T cells in plasma blood. Combining improved the differentiation. We thereby identify independently...
Neurological manifestations of autoimmune connective tissue diseases (CTD) are poorly understood and difficult to diagnose. We here aimed address this shortcoming by studying immune cell compositions in CTD patients with without neurological manifestation.Using flow cytometry, we retrospectively investigated paired cerebrospinal fluid (CSF) blood samples 28 manifestation, 38 manifestation (N-CTD), non-inflammatory controls, multiple sclerosis (MS) patients, a paradigmatic primary...