A5057534134- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Pancreatic and Hepatic Oncology Research
- Telomeres, Telomerase, and Senescence
- Acute Lymphoblastic Leukemia research
- Lymphoma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cell Adhesion Molecules Research
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Viral-associated cancers and disorders
- Extracellular vesicles in disease
- Organometallic Compounds Synthesis and Characterization
- Chronic Myeloid Leukemia Treatments
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Metal complexes synthesis and properties
- Renal cell carcinoma treatment
Universidade de Brasília
2016-2025
Memorial Sloan Kettering Cancer Center
2016-2023
Japanese Foundation For Cancer Research
2020-2021
Laboratory of Molecular Genetics
2017
Université Paris Cité
2014
Inserm
2014
Délégation Paris 5
2014
The University of Tokyo
2004-2007
We previously reported that upregulation of SMYD3, a histone H3 lysine‐4‐specific methyltransferase, plays key role in the proliferation colorectal carcinoma (CRC) and hepatocellular (HCC). In present study, we reveal SMYD3 expression is also elevated great majority breast cancer tissues. Similarly to CRC HCC, silencing by small interfering RNA this gene resulted inhibited growth cells, suggesting increased essential for cells. Moreover, show here could promote carcinogenesis directly...
Abstract We previously identified SMYD3 as a histone methyltransferase and showed that its expression was elevated in colorectal, hepatocellular, breast carcinomas. In the investigation of activity SMYD3, we have found vascular endothelial growth factor receptor 1 (VEGFR1) also methylated by SMYD3. further residue at VEGFR1 lysine 831, which is located kinase domain conserved among orthologues. followed serine, some methylation targets methyltransferases. Furthermore, enhanced cells. These...
Blood-based analysis of circulating tumor DNA (ctDNA) is a promising tool for cancer screening, monitoring relapse/recurrence and evaluating response to treatment. Although plasma widely used obtain ctDNA, biorepositories worldwide possess huge number serum samples comparative studies on the use vs as ctDNA sources are essential.We analyzed cell-free (cfDNA) from matched EDTA-plasma healthy donors patients with colorectal or lung cancer, targeted next-generation sequencing evaluate mutation...
In the search for new metal complexes with antitumor potential, two dithiocarbazate ligands derived from 1,1,1-trifluoro-2,4-pentanedione (H 2 L 1 ) and four Ni(II) complexes, [Ni(L )PPh 3 ] (1), )Py] (2), (3), (4), were successfully synthesized investigated by physical-chemistry spectroscopic methods. The crystal structure of H has been elucidated single-crystal X-ray diffraction. obtained confirms cyclization reaction formation pyrazoline derivative. results showed square planar geometry...
Elucidating the conformational heterogeneity of proteins is essential for understanding protein function and developing exogenous ligands. With rapid development experimental computational methods, it great interest to integrate these approaches illuminate landscapes target proteins. SETD8 a lysine methyltransferase (PKMT), which functions in vivo via methylation histone nonhistone targets. Utilizing covalent inhibitors depleting native ligands trap hidden states, we obtained diverse X-ray...
SMYD4 is a member of the SMYD family that has lysine methyltransferase function. Little known about roles in cancer. The aim this study to investigate genetic alterations gene across most prevalent solid tumors and determine its potential as biomarker. We performed an integrative multi-platform analysis common mutations, copy number (CNAs), mRNA expression levels genes using cohorts available at Cancer Genome Atlas (TCGA), cBioPortal, Catalogue Somatic Mutations (COSMIC). displayed lower...
Approximately two-thirds of patients with colorectal cancer (CRC) undergo resection curative intent; however, 30% to 50% these experience recurrence. The concentration cell-free DNA (cfDNA) before and after surgery may be related the prognosis CRC, but there is limited information regarding cfDNA levels at time surgery. Here, we analyzed surgical release using plasma samples from 30 three key points during surgery: preoperative (immediately surgery), intraoperative (during postoperative (at...
<title>Abstract</title> Background Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy worldwide. Despite a good rate of treatment success, poor prognosis underscores urgent need for new prognostic markers and effective therapeutic strategies. The SET family lysine methyltransferases (KMTs) has been implicated in several cancers. While <italic>SMYD2</italic> identified as marker ALL, <italic>SETD4</italic> member that still poorly characterized. Methods In present...
Pancreatic cancer is one of the cancers with very poor prognosis; there an urgent need to identify novel biomarkers improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for detection and surveillance. However, it known that ctDNA rate in resected pancreatic low compared other types cancer. In this study, we collected paired plasma samples 145 patients. Plasma were 71 patients treatment-naïve status 74 after neoadjuvant therapy...
Cancer is comprised of a multitude epigenetic abnormalities, including the global loss and regional gain DNA methylation as well alterations in histone methylation.Here, we characterize new methyltransferase, SET domain-containing protein 4 (SETD4), which involved breast carcinogenesis.Quantitative real-time PCR (qPCR) showed elevated expression levels SETD4 several cancer cell lines.SETD4 overexpression was confirmed by western blot analysis suggesting correlation between high lack estrogen...
Selective inhibitors of protein lysine methyltransferases, including SET domain-containing 8 (SETD8), are highly desired, as only a fraction these enzymes associated with high-quality inhibitors. From our previously discovered SETD8 inhibitor, we developed more potent analog and solved cocrystal structure, which is the first crystal structure in complex small-molecule inhibitor. This allowed design covalent inhibitor (MS453), specifically modifies cysteine residue near binding site, has an...
The histone lysine methyltransferases contain a SET domain, which catalyzes the addition of methyl groups to specific residues. MLL family genes encodes histone-modifying enzymes with 3-lysine 4 methyltransferase activity that can regulate gene transcription. exists in multi-protein complexes and has been implicated variety processes including normal development cell growth. Although some members have already described be involved cancer, clear relationship these breast cancer is not...
SET and MYND domain containing 2 (SMYD2) the 3 (SMYD3) are most studied well-characterized members of SMYD family. It has been demonstrated that their altered expression is associated with progression several solid tumors. Nevertheless, whether these methyltransferases exert any impact in chronic lymphocytic leukemia (CLL) remains unknown. Here, we investigated gene profile SMYD2 SMYD3 59 samples CLL 10 normal B cells. The obtained results were white blood cells (WBC) platelet counts, ZAP-70...
Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm whose pathogenesis linked to the Philadelphia chromosome presence that generates BCR-ABL1 fusion oncogene. Tyrosine kinase inhibitors (TKI) such as imatinib mesylate (IM) dramatically improved treatment efficiency and survival of CML patients by targeting BCR-ABL tyrosine kinase. The disease shows three distinct clinical-laboratory stages: chronic phase, accelerated phase blast crisis. Although in respond well treatment,...
In an attempt to disclose mechanisms of hepatocarcinogenesis and discover novel target molecules for the diagnosis treatment hepatocellular carcinomas (HCCs), we previously analyzed expression profiles HCC tissues by means human cDNA microarray. Among genes upregulated in tumor compared with their nontumor counterparts, focused on a gene, termed WDRPUH, characterized its biologic function. WDRPUH encodes predicted 620-amino acid protein containing 11 highly conserved WD40-repeat domains....
Acute Lymphoblastic Leukemia (ALL) is the predominant hematological malignancy in pediatric populations, originating from B- or T-cell precursors within bone marrow. The disease exhibits a high degree of heterogeneity, both at molecular level and terms clinical presentation. A complex interplay between inherited acquired genetic alterations contributes to pathogenesis, often resulting disruption cellular functions integral leukemogenic process. advent CRISPR/Cas9 as gene editing tool has...