A5102761571- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Malaria Research and Control
- Mosquito-borne diseases and control
- Advanced Proteomics Techniques and Applications
- Organ Donation and Transplantation
- Neonatal and Maternal Infections
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Aortic Disease and Treatment Approaches
- Pneumonia and Respiratory Infections
- Aortic aneurysm repair treatments
- Cardiac Ischemia and Reperfusion
- Mass Spectrometry Techniques and Applications
- Cardiac, Anesthesia and Surgical Outcomes
- Transplantation: Methods and Outcomes
- Mitochondrial Function and Pathology
- Neonatal Respiratory Health Research
- Advanced MRI Techniques and Applications
- Protease and Inhibitor Mechanisms
- Computational Drug Discovery Methods
- Glycosylation and Glycoproteins Research
- Anesthesia and Neurotoxicity Research
- Protein purification and stability
- Respiratory viral infections research
University of Oxford
2015-2024
Zhongnan University of Economics and Law
2024
Oxford BioMedica (United Kingdom)
2018-2024
Fujian Medical University
2023
NHS Blood and Transplant
2021
Nuffield Orthopaedic Centre
2014-2020
Centre for Human Genetics
2012-2019
National Heart Institute
2019
John Radcliffe Hospital
2017
Churchill Hospital
2015
Tregs can adopt a catabolic metabolic program with increased capacity for fatty acid oxidation–fueled oxidative phosphorylation (OXPHOS). It is unclear why this form of metabolism favored in and, more specifically, whether represents an adaptation to the environment and developmental cues or "hardwired" by Foxp3. Here we show, using analysis unbiased mass spectroscopy–based proteomics approach, that Foxp3 both necessary sufficient Treg-increased respiratory Tregs' ability utilize acids fuel...
Abstract In the present study, we used 2‐D differential gel electrophoresis (2‐D DIGE) and MS to screen biomarker candidates in serum samples obtained from 39 patients with breast cancer 35 controls. First, pooled matched age menopausal status. Then, depleted two most abundant proteins albumin IgG by immunoaffinity chromatography under partly denaturing conditions order enrich low‐abundance low molecular weight. Concentrated desalted were labeled three different CyDyes including one internal...
The voltage-gated sodium channel Na
Differentiating between self-resolving viral infections and bacterial in children who are febrile is a common challenge, causing difficulties identifying which individuals require antibiotics. Studying the host response to infection can provide useful insights lead identification of biomarkers with diagnostic potential. This study aimed identify protein for future development into an accurate, rapid point-of-care test that distinguish infections, by recruiting presenting health-care settings...
Two-dimensional gas chromatography mass spectrometry (GCxGC-MS) is utilized to an increasing extent in biomedical metabolomics. Here, we established and adapted metabolite extraction derivatization protocols for cell/tissue biopsy, serum urine samples according their individual properties. GCxGC-MS analysis revealed detection of ~600 molecular features from which 165 were characterized representing different classes such as amino acids, fatty lipids, carbohydrates, nucleotides small polar...
Lipocalin 2 distinguishes severe and bacterial pneumonia from nonsevere nonbacterial with a high level of precision. The clinical impact this biomarker requires large-scale evaluation.
Ischaemia and reperfusion injury (IRI) is the leading cause of acute kidney (AKI), which contributes to high morbidity mortality rates in a wide range injuries as well development chronic disease. The cellular molecular responses IRI are complex not fully understood. Here, we used an integrated proteomic metabolomic approach investigate effects on protein abundance metabolite levels. Rat kidneys were subjected 45 min warm ischaemia followed by 4 h 24 reperfusion, with contralateral separate...
Surgical trauma induces a complex inflammatory stress response, associated with postoperative morbidity. Patients’ physiological reserve, comorbidity, underlying disease and type of surgery will interrelate the response. In addition, different anaesthetic agents are shown to have differential effects on this We explored molecular an intermediate surgical intervention together effect either propofol or sevoflurane based anaesthesia in unique cohort healthy living kidney donors (n=36, 19, 17)...
Within this study, the potential of three clinically relevant microproteins (SE–AG–AZ, SE–EM and SE–EP) with cystine-knot architecture as pharmacophoric scaffolds for oral peptide delivery was investigated. Cystine-knot (CKM) were analysed regarding their stability towards most important gastrointestinal secreted membrane bound proteases in physiological concentrations. In addition, permeation behaviour through freshly excised rat intestinal mucosa well parameters such aggregation behaviour,...
Neuropathic pain may arise following peripheral nerve injury though the molecular mechanisms associated with this are unclear. We used proteomic profiling to examine changes in protein expression formation of hyper-excitable neuromas derived from rodent saphenous nerves. A two-dimensional difference gel electrophoresis (2D-DIGE) strategy was employed between developing and normal nerves whole tissue lysates. found around 200 proteins which displayed a >1.75-fold change neuroma identified 55...
The study of protein phosphorylation has grown exponentially in recent years, as it became evident that important cellular functions are regulated by and dephosphorylation proteins on serine, threonine tyrosine residues. use immobilized metal affinity chromatography (IMAC) to enrich phosphopeptides from peptide mixtures been shown be useful especially prior mass spectrometric analysis. For the selective enrichment applying solid-phase extraction (SPE) phosphorylated peptides, we introduce...
The successful application of-omics technologies in the discovery of novel biomarkers and targets therapeutic interventions is facilitated by large collections well curated clinical samples stored bio banks. Mining plasma proteome holds promise to improve our understanding disease mechanisms may represent a source biomarkers. However, major confounding factor for defining disease-specific proteomic signatures variation handling processing leading protein degradation. To address this, we...
Machine perfusion is rapidly becoming the standard for graft preservation and provides new opportunities organ salvage, reconditioning, repair. Based on their immune-modulatory regenerating properties,1 arguable mesenchymal stromal cells (MSCs) are most promising cell therapy repair pump, though proof of concept still lacking.2 Here, we show feasibility delivering clinically relevant numbers human MSCs (hMSCs; 5–10 × 106/kg) during 30-minute hypothermic oxygenated machine in porcine liver...
We present a simple protocol for affinity depletion to remove the two most abundant serum proteins, albumin and immunoglobulin G (IgG). Under native conditions, albumin/IgG were efficiently removed several proteins enriched as shown by two-dimensional electrophoresis (2-DE). Besides that, partly denaturing conditions established adding 5 or 20% acetonitrile (ACN) in order disrupt binding of low-molecular-weight (LMW) carrier albumin/IgG. 2-DE results showed that total number detected LMW...
Introduction: Spontaneous preterm birth (sPTB), which predominantly presents as spontaneous labor (sPTL) or premature rupture of fetal membrane (PPROM), is a syndrome that accounts for 5-10% live births annually. The long-term morbidity in surviving infants significantly higher than full-term neonates. causes sPTB are complex and not fully understood. Human placenta, the maternal interface, an environmental core intrauterine life, mediates oxygen exchange, nutrient uptake, waste elimination...
We describe a proteomics analysis to determine the molecular differences between normothermically perfused (normothermic machine perfusion, NMP) human kidneys with urine recirculation (URC) and replacement (UR). Proteins were extracted from 16 kidney biopsies URC (n = 8 donors after brain death [DBD], n circulatory [DCD]) three UR 2 DBD, 1 DCD), followed by quantitative mass spectrometry. Damage-associated patterns (DAMPs) decreased in tissue 6 hours NMP URC, suggesting reduced inflammation....
Silicone has been used in medical practice as a paradigmatic implant material for decades despite significant detrimental side effects. Our targeted proteomics approach was aimed at identification of the proteins adsorbed to surface silicone because they have characterized key components onset and perpetuation local immune reactions silicone. The composition proteinacious film, dynamics protein deposition, modifications after adsorption were analyzed both vivo vitro. Differential analysis...
Background Acute malarial anemia remains a major public health problem. Hepcidin, the hormone controlling availability of iron, is raised during acute and asymptomatic parasitemia. Understanding role mechanism hepcidin so reduced iron infection critical to establish evidence-based guidelines for management malaria anemia. Our recent clinical evidence suggests potential IL-10 in regulation patients with P. falciparum malaria. Methods We have measured secretion by primary macrophages hepatoma...
IntroductionDelayed graft function (DGF) is often defined as the need for dialysis treatment in first week after a kidney transplantation. This definition, though readily applicable, generic and unable to distinguish between "types" of DGF or time needed recover that may also significantly affect longer-term outcomes. We aimed profile biological pathways DCD donors correlate with different durations.MethodsN=30 biopsies were selected from UK Quality Organ Donation biobank stratified...
Detailed characterization of phosphoproteins as well other post-translationally modified proteins such glycoproteins, is required to fully understand protein function and regulatory events in cells organisms. Therefore, an experimental strategy for the isolation using a new immobilized metal ion affinity chromatograph (IMAC) material on basis cellulose has been developed characterized. Different approaches have used test material. Recovery rates were determined by 32P labelling myelin basic...
In an era where we are becoming more reliant on vulnerable kidneys for transplantation from older donors, there is urgent need to understand how brain death leads kidney dysfunction and, hence, this can be prevented. Using a rodent model of hemorrhagic stroke and next-generation proteomic metabolomic technologies, aimed delineate which key cellular processes perturbed in the after death. Pathway analysis signature brain-dead donors revealed large-scale changes mitochondrial proteins that...
As biocompatible matrices, porcine dermal scaffolds have limited application in tissue engineering due to rapid degradation following implantation. This study compared the physical, chemical and biomechanical changes that occurred when genipin quercetin were used crosslink determine whether could be as an alternative genipin. Physicochemical collagen assessed using spectroscopic methods [X-ray diffraction analysis (XRD) nuclear magnetic resonance (NMR) analysis]. The crosslinking reaction...