MicroRNAs (miRNA) can regulate cancer cell proliferation and metastasis. Here, we show that miR‐338‐3p is down‐regulated in metastatic tumor tissues compared to primary tumors, inhibit by inducing cycle arrest, as well restrain migration invasion. PREX2a confirmed a direct target of miR‐338‐3p. Knockdown inhibits proliferation, invasion through the PTEN/Akt pathway. miR‐338‐3p‐dependent inhibition be rescued PREXa. Overall, this study demonstrates affects pathway down‐regulating PREX2a. This...

Osteosarcoma is a malignant primary bone tumor. Bone marrow-derived mesenchymal stem cells-derived extracellular vesicles (BMSC-EVs) bear repair function for and cartilage. This study investigated the mechanism of BMSC-EVs in osteosarcoma cell proliferation, migration invasion.BMSC-EVs were isolated identified. The effects different concentrations EVs on invasion evaluated. LncRNA MALAT1 expression cells was detected. BMSCs transfected with si-MALAT1 or si-NC. binding relationships between...

Ceftriaxone (CTX) is widely used in pediatric infectious disease treatment, although the diagnostic and therapeutic management of CTX-induced gallbladder pseudolithiasis (PL) remains challenging. In this study, we investigated occurrence, clinical features, PL children. A retrospective case-control study was conducted on 185 patients receiving CTX at a single center. Data treatment regimens, imaging findings, serum biochemical parameters post-CTX therapy were analyzed. Patients classified...

Objective This study aims to identify potential biomarkers for Hepatoblastoma (HB) using bioinformatics and machine learning, explore their underlying mechanisms of action. Methods We analyzed the datasets GSE131329 GSE133039 perform differential gene expression analysis. Single-sample set enrichment analysis (ssGSEA) weighted co-expression network (WGCNA) were utilized modules linked activity. Protein-protein interaction (PPI) networks constructed hub genes, while random forest support...

Background Appendiceal Mucinous Neoplasms (AMNs) are a rare disease characterized by the accumulation of mucus within vermiform appendix and frequently misdiagnosed as appendicitis. Hence, it is crucial to consider AMNs because they have potential progress into peritoneal pseudomyxoma (PP), clinical syndrome distinguished buildup in peritoneum leading progressive abdominal pathology. Case report We present case involving 13-year-old male patient who was initially suspected having acute...

In this study, we found that the expression of miR-15a was positively correlated with neuroblastoma (NB) clinical pathological stage and negatively reversion-inducing cysteine-rich protein Kazal motifs (RECK) expression. Using enhanced green fluorescent (EGFP) reporter construct carrying 3'-UTR RECK, identified RECK as a direct target miR-15a. Suppression significantly decreased migration ability GI-LA-N SK-N-SH cell lines, whereas overexpression increased ability; these effects could be...

To explore a new model to predict the prognosis of liver cancer based on MRI and CT imaging data.A retrospective study 103 patients with histologically proven hepatocellular carcinoma (HCC) was conducted. Patients were randomly divided into training (n = 73) validation 30) groups. A total 1,217 radiomics features extracted from regions interest MR images each patient. Univariate Cox regression, Spearman's correlation analysis, Pearson's least absolute shrinkage selection operator analysis...

The management of hepatoblastoma (HB) becomes challenging when the tumor remains in close proximity to major liver vasculature (PMV) even after a full course neoadjuvant chemotherapy (NAC). In such cases, extreme resection can be considered potential option.

Abstract Background Pediatric inguinal hernia (PIH) is a common disease in children. Laparoscopic repair (LHR) has developed rapidly recent years, but there are still different opinions compared with traditional open (OHR). The purpose of this study was to compare the advantages and disadvantages LHR OHR treatment pediatric hernia. Methods We performed retrospective review all children (< 14 years) who underwent surgery center Affiliated Hospital Qingdao University from January 2015...

Maslinic acid is an active member of pentacyclic triterpenes predominantly found in dietary plants including hawthorn berries and olive fruit skins. It has been reported to show immense pharmacological biological importance anticancer property. This research was initiated explore the potential maslinic against human neuroblastoma. The effects on cellular apoptosis, ROS generation, cell migration invasion, caspase activation targeting MAPK/ERK signaling pathway were investigated....

Objective: To evaluate the usefulness of Hisense Computer Assisted Surgery System (Hisense CAS) in pre-operative surgical planning and intra-operative navigation for resection pediatric giant hepatic mesenchymal hamartoma (HMH).Methods: Five children with HMH underwent hepatectomy our hospital. Pre-operative abdominal enhanced CT was performed diagnosis treatment planning. Using DICOM files, three-dimensional reconstruction three cases operation navigation, SID carrying out precise liver...

Background: Standard liver volume (SLV) is important in risk assessment for major hepatectomy. We aimed to investigate the growth patterns of normal with age and body weight (BW) summarize formulae calculating SLV children. Methods: Overall, 792 Chinese children (<18 years age) were enrolled. Liver volumes measured using computed tomography. Correlations between BW, height (BH), surface area (BSA) analyzed. New selected from different regression models; they assessed by multicentral...

Persistent pancreaticobiliary reflux (PBR) is associated with a high risk of biliary malignancy. This study aimed to evaluate the proportion PBR in tract diseases and mechanisms by which promoted cholangiocarcinoma progression. Overall 227 consecutive patients primary disease participated this study. The amylase levels collected bile were analyzed. underlying effect high-amylase on duct epithelial cells progression source interleukin-8 (IL-8) its effects biological functions investigated....

Background Mutations in PKHD1 cause autosomal recessive Caroli disease, which is a rare congenital disorder involving cystic dilatation of the intrahepatic bile ducts. However, mutational spectrum and phenotype-genotype correlations have not yet been fully established. Methods Whole exome sequencing (WES) was performed on one twin sample with disease from Chinese family Shandong province. Routine Sanger used to validate WES carry out segregation studies. We also described mutation associated...

MicroRNAs (miRNAs or miRs) can participate in the development and progression of neuroblastoma. Many studies have indicated that miR-429 tumor development. However, mechanism underlying miR-429-mediated neuroblastoma remains largely unclear.Colony formation apoptosis assays were used to determine effect on cell proliferation. Its impact migration was determined using wound-healing Transwell assays. The target gene confirmed via western blotting luciferase reporter A nude mouse xenograft...

Neuroblastoma (NB) is considered a highly prevalent extracranial solid tumor in young children, and the upregulation of N‑myc proto‑oncogene (MYCN) closely associated with late stages NB poor prognostic outcomes. The current study was designed to evaluate effects exosomal microRNA (miRNA/miR)‑17‑5p from MYCN‑amplified cells on proliferative migratory potential non‑MYCN amplified cells. miR‑17‑5p found activate PI3K/Akt signaling cascade by targeting PTEN, overexpression promote cellular...

Necrotizing enterocolitis (NEC) is a gastrointestinal disease that tends to occur in premature infants. Some features may be associated with an increased probability preterm infants NEC will require surgical treatment. This study aimed identify the factors of treatment NEC.We retrospectively analyzed data who were hospitalized at The Affiliated Hospital Qingdao University from April 2011 2021. According treatments received, these patients divided into medical group and group. perinatal...

Computer-assisted Surgery system (CAS) is an effective medical imaging simulation tool, which widely used in preoperative planning of surgery. The objective this study to investigate the clinical application CAS pediatric mediastinal tumor resection.This retrospective investigated 74 children who underwent resection between June 2008 and 2022 at surgical center Affiliated Hospital Qingdao University Women Children's Hospital. Preoperative chest computed tomography was performed on all...

The purpose of this study was to investigate and discuss imaging methods management strategies for congenital choledochal cyst with co-existing intrahepatic dilation aberrant bile duct as well other complicated biliary anomalies.In we reviewed analyzed 72 patients cyst, ranging in age from 15 days 12 years old who were seen at our hospital during the past years, January 1993 October 2005.The image manifestation clinical significance coxisting carefully examined operation via MRCP,...

Abstract Objective Neuroblastoma (NBL) is an extra‐cranial solid tumor in children. This study was attempted to investigate the regulatory mechanism of long noncoding RNA LINC01410 (LINC01410) on NBL. Methods The expression LINC01410, miR‐506‐3p, and WEE1 NBL evaluated by quantitative real time polymerase chain reaction. proliferation colony formation ability cells were analyzed MTT assay. Flow cytometry assay executed measure apoptosis cell cycle. Dual‐luciferase reporter used detect...