A5051056178- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Retinopathy of Prematurity Studies
- Glaucoma and retinal disorders
- Ocular Oncology and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytokine Signaling Pathways and Interactions
- Extracellular vesicles in disease
- Ocular Diseases and Behçet’s Syndrome
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Retinal and Macular Surgery
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- Axon Guidance and Neuronal Signaling
- Cell Adhesion Molecules Research
- Zebrafish Biomedical Research Applications
- Immunotherapy and Immune Responses
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
University of Freiburg
2016-2025
University Medical Center Freiburg
2015-2025
Eye Center
2020-2024
Ophthalmology Clinic
2020-2024
Metropolitan University
2024
Scripps Research Institute
2015-2021
Daegu Gyeongbuk Institute of Science and Technology
2017-2018
ShanghaiTech University
2017
Cancer associated fibroblasts (CAFs) constitute an abundant stromal component of most solid tumors. Fibroblast activation protein (FAP) α is a cell surface protease that expressed by CAFs. We corroborate this expression profile immunohistochemical analysis colorectal cancer specimens. To better understand the tumor‐contextual role FAPα, we investigate how FAPα shapes functional and proteomic features CAFs using loss‐ gain‐of function cellular model systems. activity has strong impact on...
T cell-derived small extracellular vesicles (sEVs) exhibit anti-cancer effects. However, their potential should be reinforced to enhance clinical applicability. Herein, we generated interleukin-2-tethered sEVs (IL2-sEVs) from engineered Jurkat cells expressing IL2 at the plasma membrane via a flexible linker induce an autocrine effect. IL2-sEVs increased ability of CD8+ without affecting regulatory (Treg ) and down-regulated cellular exosomal PD-L1 expression in melanoma cells, causing...
Abstract Monitoring the density of corneal endothelial cells (CEC) is essential in management diseases. Its manual calculation time consuming and prone to errors. U-Net, a neural network for biomedical image segmentation, has shown promising results automated segmentation images healthy corneas good quality. The purpose this study was assess its performance “real-world” CEC (variable quality, different ophthalmologic diseases). outcome measures were: precision recall extraction CEC,...
Recent studies deciphering the transcriptional profile of choroidal neovascularization (CNV) in body donor eyes with neovascular age-related macular degeneration are limited by time span from death to preservation and associated 5′-RNA degradation. This study therefore used CNV control specimens that were formalin-fixed paraffin-embedded immediately after surgical extraction analyzed them a 3′-RNA sequencing approach. Transcriptome profiles estimate content immune stromal cells define...
Retinal neovascularization (RNV) membranes can lead to a tractional retinal detachment, the primary reason for severe vision loss in end-stage disease proliferative diabetic retinopathy (PDR). The aim of this study was characterize molecular, cellular and immunological features RNV order unravel potential novel drug treatments PDR.A total 43 patients undergoing vitrectomy PDR, macular pucker or hole (control patients) were included study. surgically removed epiretinal analyzed by RNA...
Purpose: Continuous vision loss due to vasoproliferative eye disease still represents an unsolved issue despite anti-vascular endothelial growth factor (VEGF) therapy. The impact of signal transducer and activator transcription 3 (STAT3) signaling on retinal angiogenesis its potential use as a therapeutic target remain controversial. In vitro, oncostatin M (OSM), strong STAT3 activator, possesses robust proangiogenic activity. This study investigated what extent the effects OSM translate in...
The retina consists of organized layers photoreceptors, interneurons, glia, epithelial cells, and endothelial cells. economic model supply demand used to appropriately determine cost is highly applicable the retina, in which extreme metabolic demands phototransduction are met by precisely localized designed vascular networks. Proper development maintenance these networks critical normal visual function; dysregulation characteristic several devastating human diseases, including but not...
Semaphorins are known modulators of axonal sprouting and angiogenesis. In the retina, we identified a distinct almost exclusive expression Semaphorin 3F in outer layers. Interestingly, these retinal layers physiologically avascular. Using functional vitro models, report potent anti‐angiogenic effects on both choroidal vessels. addition, human pigment epithelium isolates from patients with pathologic neovascularization retina displayed reduced 10 out 15 patients. Combined, results elucidate...
Significance The understanding of the molecular mechanisms activation and checkpoint processes has important therapeutic implications. Here, we show that interferon-γ is a master regulator for many cytokines. It operates partially by activating STAT1 signaling. However, most mechanism allows it to assume status. To do this, induces internalization gp130, common component heterodimeric cytokine receptors. Therefore, this could open whole new paradigm in cell biology.
Vascular abnormalities are a common component of eye diseases that often lead to vision loss. Vaso-obliteration is associated with inherited retinal degenerations, since photoreceptor atrophy lowers local metabolic demands and vascular support those regions no longer required. Given the degree neurovascular crosstalk in retina, it may be possible use one cell type rescue another face severe stress, such as hypoxia or genetically encoded cell-specific degenerations. Here, we show...
Oxygen-induced retinopathy (OIR) is a widely used model to study ischemia-driven neovascularization (NV) in the retina and serve proof-of-concept studies evaluating antiangiogenic drugs for ocular, as well nonocular, diseases. The primary parameters that are analyzed this mouse include percentage of with vaso-obliteration (VO) NV areas. However, quantification these two key variables comes great challenge due requirement human experts read images. Human readers costly, time-consuming,...
Purpose: To evaluate early detection of retinal hemangioblastomas (RHs) in von Hippel–Lindau disease (VHLD) with widefield optical coherence tomography angiography (wOCTA) compared to the standard care ophthalmologic VHLD screening a routine clinical setting. Methods: We conducted prospective comparisons three methods: wOCTA, ophthalmoscopy, and fluorescein (FA), which was performed only uncertain cases. The numbers detected RHs were among methods. underlying causes for lack investigated....
Limbal epithelial progenitor cells (LEPC) rely on their niche environment for proper functionality and self-renewal. While extracellular vesicles (EV), specifically small EVs (sEV), have been proposed to support LEPC homeostasis, data sEV derived from limbal like mesenchymal stromal (LMSC) remain limited, there are no studies sEVs melanocytes (LM). In this study, we isolated conditioned media of LMSC LM using a combination tangential flow filtration size exclusion chromatography...
Abstract Background To characterize different growth patterns of retinal hemangioblastomas (RHs) in patients with von Hippel-Lindau disease (VHLD) using swept-source optical coherence tomography angiography (SS-OCTA). Methods Single-center observational cross-sectional study. SS-OCTA B-scans were centered over primary treatment-naïve RHs to display the perfusion inner, middle, and outer areas (IA, MA, OA). Distinctive characterized K-means cluster analysis flow signal. Results Annual...
Abstract Background/Aims Retinal haemangioblastomas (RH) remain a major cause of visual impairment in patients with von Hippel‐Lindau (VHL) disease. Identification genotype–phenotype correlation is an important prerequisite for better management, treatment and prognosis. Methods Retrospective, single‐centre cohort study 200 VHL patients. Genetic data date onset RH, central nervous system (CNSH), pheochromocytoma/paraganglioma (PPGL), clear cell renal carcinoma (ccRCC) pancreatic...
Intravitreal injections (IVI) are the most commonly used procedure worldwide in treatment of retinal vascular diseases. This study investigates endophthalmitis rate, frequency subjective complaints and potential risk factors associated with IVI. In first part this monocentric number cases were analyzed based on routine hospital documentation 123,373 IVI procedures from 2013 to 2022. second part, following investigated through a survey involving 584 patients. From 2022 total 27 occurred...
Hereditary tumor predisposition syndromes pose a challenge for early detection and timely treatment of tumors. In von Hippel-Lindau disease, desirable personalized surveillance programs are lacking due to insufficient data on genotype-specific risk profiles individual mutations. To describe neoplastic carriers pathogenic likely VHL germline mutations, our observational study recruited 1350 participants from 40 centers worldwide. 432 different mutations were observed, with p.Asn78Ser,...
Abstract IL2 is known to induce anticancer effects by activating immune cells expressing the receptor complex (IL2R). However, potential impact of on cancer IL2R, such as melanoma cells, has been understudied. Here, we demonstrate that significantly reduces secretion small extracellular vesicles (sEVs) and expression programmed death ligand 1 (PD-L1) in while increasing their sensitivity CD8+ T cell-mediated cytotoxicity. These are mediated through activation IL2R-MEK/ERK signaling cells. To...
Aberrant angiogenesis is a hallmark of cardiovascular and retinal neovascular disease. The STAT3 signaling pathway represents potential pharmacological target for these diseases due to its impact on angiogenesis. Surprisingly, some activators, such as the IL-6 cytokine family member oncostatin M (OSM), enhance angiogenesis, whereas others, ciliary neurotropic factor (CNTF), reduce it. This study aimed clarify conflicting effects. In contrast anti-angiogenic CNTF, pro-angiogenic OSM was able...
To develop a generative adversarial network (GAN) to segment major blood vessels from retinal flat-mount images oxygen-induced retinopathy (OIR) and demonstrate the utility of these GAN-generated vessel segmentations in quantifying vascular tortuosity.
Abstract Purpose To characterize the choriocapillaris (CC) structure in relation to subretinal fluid (SRF) as a possible systematic error source using spectral domain (SD-OCTA) compared swept-source optical coherence tomography angiography (SS-OCTA). Methods This is prospective case-control study of 23 eyes. Ten patients with acute central serous chorioretinopathy (CSC), three partial macular-off retinal detachment (RD) and ten healthy, age-matched controls were included. Abnormal CC...
Purpose: Proliferative vitreoretinopathy (PVR) remains an unresolved clinical challenge and can lead to frequent revision surgery blindness vision loss. The aim of this study was characterize the microenvironment epiretinal PVR tissue, in order shed more light on complex pathophysiology unravel new treatment options. Methods: A total 44 tissue samples were analyzed study, including 19 PVRs, 13 membranes (ERMs) from patients with macular pucker, as well 12 internal limiting (ILMs). cellular...