A5063605572- Plant-derived Lignans Synthesis and Bioactivity
- Fungal Plant Pathogen Control
- Synthesis and biological activity
- ATP Synthase and ATPases Research
- Oxidative Organic Chemistry Reactions
- Protein Tyrosine Phosphatases
- Catalytic C–H Functionalization Methods
- Phytochemistry and Biological Activities
- Sulfur-Based Synthesis Techniques
- Microbial Natural Products and Biosynthesis
- Adipose Tissue and Metabolism
- Biological Activity of Diterpenoids and Biflavonoids
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Cyclopropane Reaction Mechanisms
- Radical Photochemical Reactions
- Synthetic Organic Chemistry Methods
- Reproductive tract infections research
- Galectins and Cancer Biology
- Synthesis of heterocyclic compounds
- Peptidase Inhibition and Analysis
- Synthesis of Organic Compounds
- RNA modifications and cancer
- Chemical synthesis and alkaloids
- Synthesis and Characterization of Heterocyclic Compounds
Nantong University
2016-2025
Tianjin Normal University
2025
Harbin Medical University
2015-2025
Sun Yat-sen University
2010-2025
Changchun University of Chinese Medicine
2025
Qiqihar Medical University
2025
Tianjin University of Science and Technology
2017-2024
Hebei Academy of Agriculture and Forestry Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Liaoning University of Traditional Chinese Medicine
2024
The structure of the catalytically inactive mutant (C215S) human protein-tyrosine phosphatase 1B (PTP1B) has been solved to high resolution in two complexes. In first, crystals were grown presence bis-( para -phosphophenyl) methane (BPPM), a synthetic high-affinity low-molecular weight nonpeptidic substrate ( K m = 16 μM), and was refined an R -factor 18.2% at 1.9 Å resolution. second, saturating concentration phosphotyrosine (pTyr), 18.1% 1.85 Å. Difference Fourier maps showed that BPPM...
The extracellular signal-regulated protein kinase 2 (ERK2) is the founding member of a family mitogen-activated kinases (MAPKs) that are central components signal transduction pathways for cell proliferation, stress responses, and differentiation. MAPKs unique among Ser/Thr in they require both Thr Tyr phosphorylation full activation. dual Thr-183 Tyr-185 ERK2 catalyzed by MAPK/ERK 1 (MEK1). However, identity relative activity phosphatases inactivate less well established. In this study, we...
Background and Purpose Dimethyl fumarate ( DMF ) is a newly approved drug for the treatment of relapsing forms multiple sclerosis relapsing‐remitting sclerosis. Here, we investigated effects its metabolites mono‐methylfumarate MMF methanol) on different gastrointestinal cancer cell lines underlying molecular mechanisms involved. Experimental Approach Cell viability was measured by MTT or CCK 8 assay. Protein expressions were Western blot analysis. LDH release, live‐ dead‐cell staining,...
Background/Aims: Age-related cerebrovascular dysfunction contributes to stroke, cerebral amyloid angiopathy, cognitive decline and neurodegenerative diseases. One pathogenic mechanism underlying this effect is increased oxidative stress. Up-regulation of mitochondrial uncoupling protein 2 (UCP2) plays a crucial role in regulating reactive oxygen species (ROS) production. Dietary patterns are widely recognized as contributors cardiovascular disease. In study, we tested the hypothesis that...
A stereoselective synthesis of (E)-vinyl sulfones has been developed via electrochemical oxidative N–S bond cleavage aromatic sulfonylhydrazides, followed by cross-coupling reactions with cinnamic acids to form the C–S bond. The protocol proceeded smoothly afford in good yields wide substrate scope under metal-free and halogen-free conditions.
Abstract Background Diabetic cardiovascular complications are characterised by oxidative stress-induced endothelial dysfunction. Uncoupling protein 2 (UCP2) is a regulator of mitochondrial reactive oxygen species (ROS) generation and can antagonise stress, but approaches that enhance the activity UCP2 to inhibit ROS scarce. Our previous studies show activation transient receptor potential vanilloid 1 (TRPV1) capsaicin prevent cardiometabolic disorders. In this study, we conducted experiments...
Abstract Loss of the B2M gene is associated with tumour immune escape and resistance to immunotherapy. However, genetic alterations are rare. We performed an integrative analysis mutational transcriptional profiles large cohorts non‐small‐cell lung cancer (NSCLC) patients found that epigenetic downregulation common. B2M‐low tumours exhibit a suppressive microenvironment characterized by reduced infiltration cells various lineages; in B2M‐high tumours, more T natural killer present, but their...
Protein tyrosine phosphatase 1B (PTP1B) displays a preference for peptides containing acidic as well aromatic/aliphatic residues immediately NH(2)-terminal to phosphotyrosine. The structure of PTP1B bound with DADEpYL-NH(2) (EGFR(988)(-)(993)) offers structural explanation PTP1B's [Jia, Z., Barford, D., Flint, A. J., and Tonks, N. K. (1995) Science 268, 1754-1758]. We report here the crystal structures in complex Ac-ELEFpYMDYE-NH(2) (PTP1B.Con) Ac-DAD(Bpa)pYLIPQQG (PTP1B.Bpa) determined 1.8...
Protein-tyrosine phosphatases can exhibit stringent substrate specificity in vivo, although the molecular basis for this is not well understood. The three-dimensional structure of catalytically inactive protein-tyrosine phosphate 1B (PTP1B)/C215S complexed with an optimal substrate, DADEpYL-NH2, reveals specific interactions between amino acid residues and PTP1B. goal work to rigorously evaluate functional significance Tyr46, Arg47, Asp48, Phe182, Gln262 binding catalysis, using...
Genetic variability of the nontranscribed spacer (NTS) region within ribosomal DNA repeating units in various subspecies Mus musculus was determined. Mice belonging to several laboratory mouse strains were examined by means Southern blot hybridization with a probe. This probe encompasses 3' end 28S RNA (rRNA) gene and following spacer. Restriction enzyme digestions liver DNAs from wild mice revealed that each has unique pattern encompassing distance approximately 10 kb downstream gene. These...
The synthesis of ribosomes in Saccharomyces cerevisiae consumes a prodigious amount the cell's resources and, consequently, is tightly regulated. rate ribosome responds not only to nutritional cues but also signals dependent on other macromolecular pathways cell, e.g., defect secretory pathway leads severe repression transcription both rRNA and ribosomal protein genes. A search for mutants that interrupted this revealed, surprisingly, inactivation RPL1B, one pair genes encoding 60S L1,...
IntroductionThe positive-to-resected lymph node ratio (LNR) predicts survival in many cancers, but little information is available on its value for patients with N2 NSCLC who receive postoperative radiotherapy (PORT) after resection. We tested the applicability of prognostic scoring models and heat mapping to predict overall (OS) cancer-specific (CSS) resected PORT.MethodsOur test cohort comprised identified from Surveillance, Epidemiology, End Results database received resection PORT...
Asporin (ASPN), a member of small leucine-rich repeat proteoglycan (SLRP) family proteins, serves important roles in diverse biological responses and disease conditions. We tested the hypothesis that ASPN regulated proliferation gastric cancer (GC) cells identified its down-stream regulators. promoted GC cells. effector this effect as proteasome 26S subunit non-ATPase 2 (PSMD2) which is known to regulate through suppression DUSP7, WIP1 PTEN then inducing phosphorylation ERK, P38 AKT. PSMD2...
Intermolecular carboselenenylation of easily accessible alkenes by utilizing diselenides and N-fluorobenzenesulfonimide (NFSI) under metal-free mild conditions is reported. Preliminary mechanistic studies indicate that the oxidation diselenide NFSI through a single-electron-transfer process produces an active selenenyl cationic radical species initiates intermolecular olefins, forming key Se-C C-C bonds. Under optimized conditions, broad spectrum functionally structurally diverse selenoether...
Recent advancements in contemporary therapeutic approaches have increased the survival rates of lung cancer patients; however, long-term benefits remain constrained, underscoring pressing need for novel biomarkers. Surfactant-associated 3 (SFTA3), a long non-coding RNA predominantly expressed normal epithelial cells, plays crucial role development. Nevertheless, its function adenocarcinoma (LUAD) remains inadequately understood. Single-cell sequencing data were utilized to identify...
Abstract Accumulating evidence indicates that metabolic enzymes can directly couple signals to transcriptional adaptation and cell differentiation. Glycogen synthase 1 (GYS1), the key enzyme for glycogenesis, is a nucleocytoplasmic shuttling protein compartmentalized in cytosol nucleus. However, spatiotemporal regulation biological function of nuclear GYS1 (nGYS1) microcompartments remain unclear. Here, we show dynamically reorganizes into condensates under conditions glycogen depletion or...
Acetylpyranuloses and β-oxodithioesters, recognized as notable electrophiles trinucleophiles, are frequently employed in intricate cyclization reactions. Nevertheless, there is a lack of literature documenting the products derived from these two reagents. This paper unveils base-mediated sequential Michael addition SN2-type reactions involving acetylpyranuloses β-oxodithioesters. A total 23 functionalized bicyclic molecules featuring thieno[2,3-b] pyran scaffold have been obtained...
We have developed, for the first time, a visible light-induced radical cascade difluoromethylation/cyclization reaction of 1-acryloyl-2-cyanoindoles with [bis(difluoroacetoxy)iodo]benzene under catalyst-free conditions. The use shelf-stable and easily accessible difluoromethylation reagents, combined environmentally friendly mild conditions, makes this method an alternative strategy synthesis difluoroalkylated pyrrolo[1,2-a]indolediones.
Alterations in liver metabolism play a pivotal role the development and progression of metabolic dysfunction-associated steatotic disease (MASLD). Asprosin is reported to be released from white adipose tissue during fasting targets liver. However, asprosin, especially organs other than tissue, MASLD remains poorly understood. These findings demonstrate that plasma asprosin levels are significantly elevated patients animal models. Additionally, expression increased mice. Hepatocyte-specific...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic function of the conserved hydroxyl group in protein tyrosine phosphatase signature motifZhong-Yin Zhang, Bruce A. Palfey, Li Wu, and Yu ZhaoCite this: Biochemistry 1995, 34, 50, 16389–16396Publication Date (Print):December 19, 1995Publication History Published online1 May 2002Published inissue 19 December 1995https://pubs.acs.org/doi/10.1021/bi00050a020https://doi.org/10.1021/bi00050a020research-articleACS PublicationsRequest reuse...