A5076285274- Radiation Therapy and Dosimetry
- Spectroscopy Techniques in Biomedical and Chemical Research
- DNA Repair Mechanisms
- Spectroscopy and Chemometric Analyses
- Effects of Radiation Exposure
- Boron Compounds in Chemistry
- Nuclear Physics and Applications
- Radiation Effects and Dosimetry
- Advanced Radiotherapy Techniques
- Bee Products Chemical Analysis
- Laser-Plasma Interactions and Diagnostics
- BRCA gene mutations in cancer
- Carcinogens and Genotoxicity Assessment
- Plant Genetic and Mutation Studies
- Radiation Detection and Scintillator Technologies
- Electromagnetic Fields and Biological Effects
- Laser-induced spectroscopy and plasma
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Nutrition, Genetics, and Disease
- High-pressure geophysics and materials
- Ion-surface interactions and analysis
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
University of Naples Federico II
2016-2025
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2014-2025
Istituto Nazionale di Fisica Nucleare
2005-2024
Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud
2017
Queen's University Belfast
2014
Sidney Kimmel Comprehensive Cancer Center
2014
Johns Hopkins University
2014
Queensland University of Technology
2014
Federico II University Hospital
2005-2014
University of Wales
2000
Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. functions maintain genomic stability through the assembly multiple protein complexes involved in DNA repair, cell-cycle arrest, transcriptional regulation. Here, we report identification damage-induced complex containing BCLAF1 other key components mRNA-splicing machinery. In response damage, this regulates pre-mRNA splicing number genes damage signaling thereby promoting these transcripts/proteins....
Germline mutations in BRCA1 predispose carriers to a high incidence of breast and ovarian cancers. functions maintain genomic stability through critical roles DNA repair, cell-cycle arrest, transcriptional control. A major question has been why loss or mutation leads tumors mainly estrogen-regulated tissues, given that essential all cell types. Here, we report estrogen metabolites can cause double-strand breaks (DSB) receptor-α-negative cells is required repair these DSBs prevent...
Abstract Protontherapy is hadrontherapy’s fastest-growing modality and a pillar in the battle against cancer. Hadrontherapy’s superiority lies its inverted depth-dose profile, hence tumour-confined irradiation. Protons, however, lack distinct radiobiological advantages over photons or electrons. Higher LET (Linear Energy Transfer) 12 C-ions can overcome cancer radioresistance: DNA lesion complexity increases with LET, resulting efficient cell killing, i.e. higher Relative Biological...
mRNA splicing and export plays a key role in the regulation of gene expression, with recent evidence suggesting an additional layer expression cellular function through selective genes within specific pathways. Here we describe for RNA processing factors THRAP3 BCLAF1 DNA damage response (DDR) pathway, pathway involved maintenance genomic stability prevention oncogenic transformation. We show that loss and/or leads to sensitivity damaging agents, defective repair instability. Additionally,...
The main direction proposed by the community of experts in field laser-driven ion acceleration is to improve particle beam features (maximum energy, charge, emittance, divergence, monochromaticity, shot-to-shot stability) order demonstrate reliable and compact approaches be used for multidisciplinary applications, thus, principle, reducing overall cost a laser-based facility compared conventional accelerator one and, at same time, demonstrating innovative more effective sample irradiation...
Protontherapy is a rapidly expanding radiotherapy modality where accelerated proton beams are used to precisely deliver the dose tumor target but generally considered ineffective against radioresistant tumors. Proton-Boron Capture Therapy (PBCT) novel approach aimed at enhancing biological effectiveness. PBCT exploits nuclear fusion reaction between low-energy protons and 11 B atoms, i.e. p+ B→ 3α (p-B), which supposed produce highly-DNA damaging α-particles exclusively across...
The aim of this preliminary investigation was to assess whether human peripheral blood lymphocytes which have been pre-exposed non-ionizing radiofrequency fields exhibit an adaptive response (AR) by resisting the induction genetic damage from subsequent exposure ionizing radiation. Peripheral four healthy donors were stimulated with phytohemagglutinin for 24 h and then exposed 20 1950 MHz (RF, dose, AD) at average specific absorption rate 0.3 W/kg. At 48 h, cells subjected a challenge dose...
The ability of Fourier transform infrared (FTIR) spectroscopy in analyzing cells at a molecular level was exploited for investigating the biochemical changes induced protein, nucleic acid, lipid, and carbohydrate content after irradiation by graded X-ray doses. Infrared spectra from vitro SH-SY5Y neuroblastoma following exposure to X-rays (0, 2, 4, 6, 8, 10 Gy) were analyzed using ratiometric approach evaluating ratios between absorbance significant peaks. spectroscopic investigation...
The ELIMAIA-ELIMED beamline, powered by the L3 HAPLS petawatt laser, enables irradiation of biological samples with intermediate-energy laser-driven protons (LDP) in a multi-shot regime. In pilot radiobiological experiment, mean energy ∼24 MeV and doses up to ∼14 mGy per shot, ∼4 ns bunch duration, were used irradiate AG01522 normal human skin fibroblasts. shortest time achieved was down ∼17 min/Gy, while peak dose rates reached ∼1 × 10 −3 3.5 6 Gy/s, respectively. cells exposed ranging from...
Evidence has accumulated that radiation induces a transmissible persistent destabilization of the genome, which may result in effects arising progeny irradiated but surviving cells. An enhanced death rate among cells irradiation persists for many generations form reduced plating efficiency. Such delayed reproductive is correlated with an increased occurrence micronuclei. Since it been suggested radiation-induced chromosomal instability might depend on quality, we investigated alpha particles...
Durante, M., George, K., Gialanella, G., Grossi, La Tessa, C., Manti, L., Miller, J., Pugliese, Scampoli, P. and Cucinotta, F. A. Cytogenetic Effects of High-Energy Iron Ions: Dependence on Shielding Thickness Material. Radiat. Res. 164, 571–576 (2005).We report results for chromosomal aberrations in human peripheral blood lymphocytes after they were exposed to high-energy iron ions with or without shielding at the HIMAC, AGS NSRL accelerators. Isolated energies between 200 5000 MeV/ nucleon...
This work aimed at measuring cell-killing effectiveness of monoenergetic and Spread-Out Bragg Peak (SOBP) carbon-ion beams in normal tumour cells with different radiation sensitivity. Clonogenic survival was assayed human cell lines exhibiting radiosensitivity to X- or γ-rays following exposure (incident LET 13-303 keV/μm) various positions along the ionization curve a therapeutic beam, corresponding three dose-averaged (LETd) values (40, 50 75 keV/μm). Chinese hamster V79 were also used....
Shielding is the only practical countermeasure for exposure to cosmic radiation during space travel. It well known that light, hydrogenated materials, such as water and polyethylene, provide best shielding against radiation. Kevlar Nextel are two materials of great interest spacecraft because their ability protect human infrastructures from meteoroids debris. We measured response simulated heavy-ion these compared it Lucite (PMMA), aluminum. As proxy galactic nuclei we used 1 GeV n iron or...
A micro-Raman spectroscopy investigation has been performed in vitro on single human mammary epithelial cells after irradiation by graded x-ray doses. The analysis principal component (PCA) and interval-PCA (i-PCA) methods allowed us to point out the small differences Raman spectra induced irradiation. This experimental approach enabled delineate radiation-induced changes protein, nucleic acid, lipid, carbohydrate content. In particular, dose dependence of PCA i-PCA components analyzed. Our...
Accelerated proton beams have become increasingly common for treating cancer. The need cost and size reduction of particle accelerating machines has led to the pioneering investigation optical ion acceleration techniques based on laser-plasma interactions as a possible alternative. Laser-matter interaction can produce extremely pulsed bursts ultra-high dose rates (⩾ 109 Gy/s), largely exceeding those currently used in conventional therapy. Since biological effects ionizing radiation are...
Plant polyphenols are important components of human diet and a number them considered to possess chemo-preventive therapeutic properties against cancer. They recognized as naturally occurring antioxidants, but also pro-oxidant, pro-apoptotic, or chromosomal aberrations inducers, depending on their concentration and/or the stage cell-cycle cells with which they interact. For these reasons, particular interest is devoted knowing total effects cell cycle metabolism. Fourier-Transform Infrared...
ELI-Beamlines is one of the pillars pan-European project ELI (Extreme Light Infrastructure). It will be an ultra high-intensity, high repetition-rate, femtosecond laser facility whose main goal generation and applications high-brightness X-ray sources accelerated charged particles in different fields. Particular care devoted to potential applicability laser-driven ion beams for medical treatments tumors. Indeed, such kind show very interesting peculiarities and, moreover, based accelerators...
Valerian salad and lettuce are edible species that easy to grow rapidly, have traits useful for commercial purposes. The consumption of these is increasing worldwide their nutritional properties. Seed germination seedling development critical stages in the life cycle plants. priming, including use high-energy radiation, a set techniques based on idea low stress levels stimulate plant responses, thereby improving seed growth. In this study, we evaluated hydroponic culture (i) performance;...
Manti, L., Braselmann, H., Calabrese, M. Massa, R., Pugliese, M., Scampoli, P., Sicignano, G. and Grossi, Effects of Modulated Microwave Radiation at Cellular Telephone Frequency (1.95 GHz) on X-Ray-Induced Chromosome Aberrations in Human Lymphocytes In Vitro. Radiat. Res. 169, 575–583 (2008).The case for a DNA-damaging action produced by radiofrequency (RF) signals remains controversial despite extensive research. With the advent Universal Mobile Telecommunication System (UMTS) number...