A5039116026- Sphingolipid Metabolism and Signaling
- Adenosine and Purinergic Signaling
- Paraoxonase enzyme and polymorphisms
- Eicosanoids and Hypertension Pharmacology
- Neurological Complications and Syndromes
- Biomedical Research and Pathophysiology
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hormonal Regulation and Hypertension
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Renin-Angiotensin System Studies
- Nanoparticles: synthesis and applications
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Pomegranate: compositions and health benefits
- Pancreatitis Pathology and Treatment
- Nitric Oxide and Endothelin Effects
- Cholesterol and Lipid Metabolism
- Phytochemistry and biological activity of medicinal plants
- Caveolin-1 and cellular processes
- Vagus Nerve Stimulation Research
- Diet, Metabolism, and Disease
Instituto Nacional de Cardiología
2014-2024
Instituto Nacional de Cardiologia
2021
Universidad Nacional Autónoma de México
2004
Significance Statement The calcium-sensing receptor (CaSR) activates salt reabsorption through the sodium-chloride cotransporter (NCC) via WNK4-SPAK pathway. Glucose and other sugars are positive allosteric modulators of CaSR. This would be irrelevant for most cells that come into contact with glucose, except nephron segments beyond proximal tubule. Using in vitro vivo models, this work shows arrival glucose or fructose to distal modulates CaSR due a type II effect associated activation...
Food-grade titanium dioxide (E171) and zinc oxide nanoparticles (ZnO NPs) are common food additives for human consumption. We examined multi-organ toxicity of both compounds on Wistar rats orally exposed 90 days. Rats were divided into three groups: (1) control (saline solution), (2) E171-exposed, (3) ZnO NPs-exposed. Histological examination was performed with hematoxylin–eosin (HE) staining transmission electron microscopy (TEM). Ceramide (Cer), 3-nitrotyrosine (NT), lysosome-associated...
Unique situations in female physiology require volume retention. Accordingly, a dimorphic regulation of the thiazide-sensitive Na(+)-Cl(-) cotransporter (NCC) has been reported, with higher activity females than males. However, little is known about hormones and mechanisms involved. Here, we present evidence that estrogens, progesterone, prolactin stimulate NCC expression phosphorylation. The sex difference abundance, however, species dependent. In rats, phosphorylation males, while mice...
Metallic nanoparticles (NPs) are widely used as food additives for human consumption. NPs reach the bloodstream given their small size, getting in contact with all body organs and cells. have adverse effects on respiratory intestinal tract; however, few studies focused toxic consequences of orally ingested metallic cardiovascular system. Here, two food-grade system were analyzed.
High-density lipoproteins (HDLs) are known to enhance vascular function through different mechanisms, including the delivery of functional lipids endothelial cells. Therefore, we hypothesized that omega-3 (n-3) eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) content HDLs would improve beneficial effects these lipoproteins. To explore this hypothesis, performed a placebo-controlled crossover clinical trial in 18 hypertriglyceridemic patients without symptoms coronary heart disease who...
Deleterious effects of purinergic P2X1 and P2X7 receptors (P2XRs) in ANG II-dependent hypertension include increased renal vascular resistance, impaired autoregulation pressure natriuresis. However, their specific on the determinants glomerular hemodynamics remain incompletely delineated. To investigate P2XR contributions to altered hypertension, acute blockade P2X1R, P2X7R, P2X4R with NF449, A438079, PSB12054, respectively, were evaluated II-infused rats (435 ng·kg-1·min-1). P2X1R or P2X7R...
Nonalcoholic fatty liver disease (NAFLD) is considered a manifestation of metabolic syndrome (MS) and characterized by the accumulation triglycerides varying degree hepatic injury, inflammation, repair. Moreover, peroxisome-proliferator-activated receptors (PPARs) play critical role in pathophysiological processes liver. There extensive evidence beneficial effect polyphenols such as resveratrol (RSV) quercetin (QRC) on treatment pathology; however, mechanisms underlying their effects have...
The profile of sphingomyelin and its metabolites shows changes in the plasma, organs, tissues patients with cardiovascular, renal, metabolic diseases. objective this study was to investigate effect empagliflozin on levels metabolites, as well activity acid neutral sphingomyelinase (aSMase nSMase) ceramidase (nCDase) kidney, heart, liver streptozotocin-induced diabetic Angiotensin II (Ang II)-induced hypertension rats. Empagliflozin treatment decreased hyperglycemia rats whereas blood...
(1) Background: the composition of high-density lipoproteins (HDL) becomes altered during postprandial state, probably affecting their functionality vis-à-vis endothelium. Since acute coronary syndrome (ACS) in women is frequently associated with endothelial dysfunction, it likely that HDL are unable to improve artery vasodilation these patients. Therefore, we characterized from ACS fasting and conditions. We also determined whether microencapsulated pomegranate (MiPo) reverts abnormalities,...
Purinergic receptors play a central role in the renal pathophysiology of angiotensin II-induced hypertension, since elevated ATP chronically activates P2X7 this model. The changes induced by P2X antagonist Brilliant blue G (BBG) glomerular hemodynamics and tubulointerstitial inflammation resulting from II infusion were studied. Rats received (435 ng kg−1 min−1, 2 weeks) alone or combination with BBG (50 mg/kg/day intraperitoneally). did not modify hypertension (214.5 ± 1.4 vs. 212.7 0.5...
The possibility that angiotensin II (ANG II) exerts its effects through the activation of neutral sphingomyelinase (nSMase) has not been tested in kidneys. results present study provide evidence for activity and expression nSMase rat In isolated perfused kidney, ANG II-induced renal vasoconstriction was inhibited by GW4869, an inhibitor nSMase. We used investigating signal transduction downstream ceramide. constricted vasculature. An ceramidase (CDase), N-oleoylethanolamine (OEA), enhanced...
Sphingolipids (sphingomyelin, glycolipids, and gangliosides) are located in cell membranes, plasma, lipoproteins.In patients with cardiovascular, renal, metabolic diseases, the profile of sphingolipids their metabolites (ceramide, sphingosine, sphingosine-1-phosphate) is modified, these changes may explain alterations some cellular responses such as apoptosis.Furthermore, sphingosine sphingosine-1-phosphate have been suggested to prevent COVID-19.This review also briefly mentions techniques...
Abstract The catabolism and structure of high‐density lipoproteins (HDL) may be the determining factor their atheroprotective properties. To better understand role kidney in HDL catabolism, here we characterized subclasses catabolic rates apo A‐I a rabbit model proteinuria. Proteinuria was induced by intravenous administration doxorubicin New Zealand white rabbits ( n = 10). size subclass lipids were assessed electrophoresis isolated lipoproteins. rate HDL‐apo evaluated exogenous...
It has been suggested that spinach methanolic extract (SME) inhibits the formation of advanced glycation end products (AGEs), which are increased during diabetes progression, so it is important to know if SME beneficial effects in diabetic retina. In this study, vitro assays showed glycation, carbonyl groups formation, and reduced-thiol depletion bovine serum albumin incubated either reducing sugars or methylglyoxal. The effect retinas streptozotocin-induced rats (STZ) was also studied (n =...
In this study, we investigated whether severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein may modify angiotensin-converting enzyme 2 (ACE2) activity in the plasma, heart, kidney, liver, lung, and six brain regions (amygdala, stem, cortex, hippocampus, hypothalamus, striatum) of diabetic hypertensive rats. We determine ACE2 plasma lysates regions. MLN-4760 inhibits all organs. On other hand, soluble (sACE2) increased rats, there was no change augmented striatum, while...
Previous studies demonstrated that pomegranate, which is a source of several bioactive molecules, induces modifications high-density lipoproteins (HDL) lipid composition and functionality. However, it remains unclear whether the beneficial effects pomegranate are related to improvement in components HDL. Therefore, this placebo-controlled study, we characterized size HDL subclasses assessed functionality these after 30 days supplementation with microencapsulated (MiPo) New Zealand white...
Abstract The potential cause–effect relationship between uric acid plasma concentrations and HDL functionality remains elusive. Therefore, this study aimed to explore the effect of oxonic (OA)‐induced hyperuricemia on size distribution, lipid content subclasses, apo AI turnover, as well in New Zealand white rabbits. Experimental animals received OA 750 mg/kg/day by oral gavage during 21 days. HDL‐apo fractional catabolic rate (FCR) was determined exogenous labeling with 125 I, subclasses...