A5073617706- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Gastric Cancer Management and Outcomes
- Circular RNAs in diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Reproductive System and Pregnancy
- Genetic factors in colorectal cancer
- Helicobacter pylori-related gastroenterology studies
- Orthopaedic implants and arthroplasty
- Gastrointestinal Tumor Research and Treatment
- Ectopic Pregnancy Diagnosis and Management
- Cancer-related gene regulation
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Mycobacterium research and diagnosis
- DNA Repair Mechanisms
- Colorectal Cancer Surgical Treatments
- PI3K/AKT/mTOR signaling in cancer
- Endometriosis Research and Treatment
- Signaling Pathways in Disease
- Pancreatic and Hepatic Oncology Research
- Spectroscopy Techniques in Biomedical and Chemical Research
Second Affiliated Hospital of Nanjing Medical University
2014-2024
Nanjing Medical University
2017-2020
State Council of the People's Republic of China
2017-2020
Wuhan University
2012-2020
Renmin Hospital of Wuhan University
2012-2020
Affiliated Hospital of Nantong University
2020
Nantong University
2020
Medical Technologies (Czechia)
2017
Zhangqiu City People's Hospital
2015
Nantong Science and Technology Bureau
2014
Emerging evidence has shown that dysregulation function of long non-coding RNAs (lncRNAs) implicated in gastric cancer (GC). However, the role differentially expressed lncRNAs GC not fully explained.LncRNA expression profiles were determined by lncRNA microarray five pairs normal and tissues, further validated another 75 paired tissues quantitative real-time PCR (qRT-PCR). Overexpression MT1JP was conducted to assess effect vitro vivo. The biological functions demonstrated luciferase...
Abstract The phenomenon of stress cracking Pellethane 2363‐80A (PEU) was investigated using the cage implant system. A cytotoxic polyvinylchloride (PVC) and a silicone rubber containing an anti‐inflammatory steroid were used to create inflammatory environments in which biostability pre‐stressed PEU tested. These coimplants provided alternative vivo study polymer interactions. responses implanted cages monitored by analyzing exudates aspirated from at different implantation times over 21...
Long non-coding RNA (lncRNA) is emerging as an critical regulator in multiple cancers, including pancreatic cancer (PC). Recently, lncRNA SNHG15 was found to be up-regulated gastric and hepatocellular carcinoma, exerting oncogenic effects. Nevertheless, the biological function regulatory mechanism of remain unclear In this study, we reported that expression also upregulated PC tissues, its overexpression remarkably associated with tumor size, node metastasis (TNM) stage lymph patients PC....
The first report of potential mechanisms IncRNA SNHG17 in CRC, prompting the development IncRNA-directed diagnosis and treatments.
Abstract Our previous genome-wide miRNA microarray study revealed that miR-107 was upregulated in gastric cancer (GC). In this we aimed to explore its biological role the pathogenesis of GC. Integrating silico prediction algorithms with western blotting assays inhibition enhanced NF1 (neurofibromin 1) mRNA and protein levels, suggesting is one targets Luciferase reporter assay suppressed expression by binding first potential site within 3′-UTR mRNA. stable indicated could result instability,...
Intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) activated by HCG in vitro is reported to improve implantation rates patients with repeated failure IVF-ET. In this article, the impact intrauterine PBMCs on embryo implantation, pregnancy rate and underlying mechanisms will be investigated.Pregnant mice were randomly divided into three groups, including control group; dysfunction (EID) EID group. Uterine horns excised determine number pregnant sites Day 7.5...
Background In the past decades, a good deal of studies has provided possibility circulating microRNAs (miRNAs) as noninvasive biomarkers for cancer diagnosis. The aim our study was to detect levels miRNAs in tissues and plasmas gastric (GC) patients evaluate their diagnostic value. Methods Tissue samples were collected from 85 GC patients. Plasma 285 matched controls. Differentially expressed filtered with by Agilent Human miRNA Microarray TaqMan low density array (TLDA) pooled samples,...
In our previous study, we demonstrated that four microRNAs (miRNAs) (miR-26a, miR-142-3p, miR-148a, and miR-195) were downregulated in both plasma tumor tissues confirmed to be promising non-invasive diagnostic biomarkers for gastric cancer (GC).We used the quantitative reverse transcription polymerase chain reaction assess expression levels of miRNAs from paraffin-embedded surgical specimens GC patients. Kaplan-Meier curves log-rank test applied predict correlation between cumulative...
Embryo quality is crucial to the outcome of in vitro fertilization (IVF); however, ability precisely distinguish embryos with higher reproductive potential from others poor. Morphologic evaluation used play an important role assessing embryo quality, but it somewhat subjective. The culture medium immediate environment vitro, and a change substances possibly related quality. Thus, present study aims determine whether metabolomic profiling using Raman spectroscopy adjunct morphology correlates...
Inactivation of tumor suppressor genes by promoter hypermethylation plays a key role in the tumorgenesis. It is necessary to uncover detailed pattern whole genome-wide abnormal DNA methylation during development gastric cancer (GC). We performed detection using 12 paired GC tissues and their corresponding normal tissues. Methylation-specific PCR (MSP) bisulphite sequencing (BSP) were used measure status specific CpG site. Based on bioinformatic analysis, cell phenotypes mouse model...
Environmental stress cracking (ESC) in polyetherurethanes has been demonstrated animal models. However, duplication of this phenomenon vitro a challenge. The glass wool-H2O2/CoCl2 test system was designed to provide oxidation components found vivo, i.e., hydroxyl radical (HO.), molecular oxygen (O2), and superoxide (O2-.) required for auto-oxidation polyetherurethanes. is not only reliable duplicating the characteristics vivo at 37 degrees C, but also accelerates ESC as much seven times....
As an imperative part of PI3K/Akt/mTOR pathway, mammalian target rapamycin (mTOR) has been demonstrated to increase in gastric cancer cells and tumors. Our research explored the relationship between single nucleotide polymorphism (SNP) rs2295080 mTOR promoter region risk (GC).Seven hundred fifty-three (753) adenocarcinoma patients 854 matched healthy subjects were recruited association study 60 tissues used test expression mTOR. Unconditional logistic regression was selected evaluate T>G GC...
Aim: Natural killer (NK) cells, as key regulatory accumulate at the maternal-fetal interface in large numbers. This study explored effect of miR-30e on regulating activity and function peripheral blood NK cells (PB-NK cells) decidua (D-NK by targeting PRF1 immune tolerance interface. Methods: Expressions PB tissues from 49 patients with recurrent spontaneous abortion 52 normal pregnant women were measured using PCR. isolated identified flow cytometry (FCM). In PB-NK D-NK activated IFN-α,...
Background Previous genome-wide association studies (GWASs) have identified that several single nucleotide polymorphisms (SNPs) are implicated in gastric cancer (GC) risk. However, the multiple statistical comparisons of GWASs may reject some true biological positives with subthreshold P values. Methods This study annotated genomic locations all CpG islands genome using Encyclopedia DNA Elements (ENCODE). The SNPs regions were then genotyped Illumina 660W Quad chip. effects prominent...
In spite of the achievement in treatment, gastric cancer (GC) mortality still remains high. MicroRNAs (miRNAs) are a group small noncoding RNAs that play crucial part tumor progression. this study, we explored expression and function microRNA-501-5p (miR-501-5p) GC cell lines. Quantitative real-time polymerase chain reaction assay results suggested miR-501-5p was significantly upregulated tissues And, Cell Counting Kit-8 colony formation migration showed downregulation decreased...
The integrated analytical strategy identified both genetic and epigenetic biomarkers for gastric cancer etiology.
Abstract Endometriosis is considered a benign gynaecological disease with cancer‐like characterizations, which has high incidence among women of reproductive age. However, this so far lacked timely diagnosis and effective treatment owing to its unclear aetiology. In study, we identified aberrant expression circ_0007331 in ectopic endometrial cells by comparing the samples from patients without endometriosis. Further functional experiments revealed that knock‐down effectively suppressed...
Gastric cancer (GC) is one of the most common human malignancies worldwide, but molecular mechanism GC has not been fully elucidated. Tetraspanin 31 (TSPAN31) rarely studied in malignant tumors. This study aimed to investigate effects TSPAN31 on GC. We analyzed tissues through high-throughput sequencing technology and chose with high expression. The expression was bioinformatics website qRT-PCR. protein level determined by western blot immunochemistry. proliferation, migration, apoptosis...
Single nucleotide polymorphisms (SNPs) in the 3′-untranslated regions targeted by putative mircoRNA can change its binding strength, affecting susceptibility and prognosis of cancer. We aimed to investigate associations between SNPs within miR-148a sites gastric cancer (GC) risk prognosis. Using bioinformatics tools, we selected two (SCRN1 rs6976789 PDYN rs2235749) located target sites. genotyped a case-control study comprising 753 GC patients 949 cancer-free subjects. found significantly...