A5011569846- Immune cells in cancer
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Heat shock proteins research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Asthma and respiratory diseases
- interferon and immune responses
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Blood Coagulation and Thrombosis Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Phosphodiesterase function and regulation
- SARS-CoV-2 and COVID-19 Research
- Inhalation and Respiratory Drug Delivery
- Signaling Pathways in Disease
- Mast cells and histamine
University of Brescia
2014-2024
Brescia University
2007-2021
Università Cattolica del Sacro Cuore
2020
University of Milan
1999
Sanofi (United States)
1995
Sterling Research Group
1992
The inflammatory and IFN pathways of innate immunity play a key role in the resistance pathogenesis coronavirus disease 2019 (COVID-19). Innate sensors SARS-CoV-2-associated molecular patterns (SAMPs) remain to be completely defined. Here, we identified single-stranded RNA (ssRNA) fragments from SARS-CoV-2 genome as direct activators endosomal TLR7/8 MyD88 pathway. same sequences induced human DC activation terms phenotype function, such cytokine production Th1 polarization. A bioinformatic...
Abstract: In the liver, CC1 4 induces cell necrosis followed by regeneration. Cell injury is caused free radical damage and may be due, at least in part, to oxidative stress subsequent formation of reactive oxygen intermediates (ROIs). a rat model acute CCl ‐induced hepatic injury, we examined expression genes involved cellular response different kinds stress, including (hsp70 family, heme oxygenase), detoxification (Mn superoxide dismutase Cu/Zn dismutase), iron homeostasis (H L ferritin...
Basic fibroblast growth factor (bFGF) induces a protein kinase C (PKC)-dependent mitogenic response in transformed fetal bovine aortic endothelial GM 7373 cells. A long-lasting interaction of bFGF with the cell is required to induce proliferation. bFGF-treated cells are fact committed proliferate only after they have entered phase S cycle, 12-14 h beginning treatment. Before that time, abolished by 1) removal extracellular suramin, 2) addition neutralizing anti-bFGF antibodies culture...
Angiogenesis plays a crucial role in tumor growth and progression. Low expression of mineralocorticoid receptor (MR) several malignant tumors correlates with disease recurrence overall survival. Previous studies have shown that MR is decreased colorectal cancer (CRC). Here we hypothesize can contribute to angiogenesis poor patient survival malignancies. In cohort CRC patients, analyzed expression, its correlation microvascular density impact on Subsequently, interrogated the an vitro model,...
Background: Cigarette smoke extract (CSE), a model for studying the effects of tobacco in vivo and vitro, induces cell oxidative stress affects antioxidative glutathione system. We evaluated impact CSE on airway epithelial cells possible cytoprotective effect mucolitic drug S-carboximethilcysteine lysine salt (S-CMC-Lys). Methods: Reduced (GSH) reactive oxygen species (ROS) intracellular levels were by fluorimetry human bronchial (16-HBE) expression activity enzymes GSH metabolic pathway...
COVID-19 disease is characterized by a dysregulation of the innate arm immune system. However, mechanisms whereby cells, including neutrophils, become activated in patients are not completely understood. Recently, we showed that GU-rich RNA sequences from SARS-CoV-2 genome (i.e., SCV2-RNA1 and SCV2-RNA2) activate dendritic cells. To clarify whether human neutrophils may also represent targets SCV2-RNAs, were treated with either SCV2-RNAs or, as control, R848 (a TLR7/8 ligand), then analyzed...
Abstract In vitro experiments performed by several investigators have demonstrated that IL-7 is a growth factor for immature B lymphocytes, thymocytes, and mature T lymphocytes. To evaluate the potential therapeutic use human rIL-7 (rhuIL-7) as hematopoietin, we studied in vivo hematopoietic effects of rhuIL-7 mice. these experiments, sublethally irradiated normal mice were treated with or without up to 26 days. Administration significantly increased white blood cell count peripheral spleen...
Two groups of prolactinoma cell lines were identified. One group (responder) expresses both D2 dopamine receptors and an autocrine loop mediated by nerve growth factor (NGF) one (nonresponder) lacks NGF production. receptor expression in these is dependent on NGF. Indeed, inactivation responder cells decreases density, while treatment induces nonresponders. Here we show that p75NGFR, but not trkA, resulted loss prevented induced the nonresponder. Analysis nuclear factor-κB (NF-κB)...
The contribution to airflow obstruction by the remodeling of peripheral airways in chronic obstructive pulmonary disease (COPD) patients has been well documented, but less is known about role played large airways. Few studies have investigated presence histopathological changes due COPD patients.The aim this study was verify airway central patients, quantifying smooth muscle (ASM) area and extracellular matrix (ECM) protein deposition, both subepithelial region ASM, possible above mentioned...
Patterns of receptors for chemotactic factors regulate the homing leukocytes to tissues. Here we report that CCRL2/chemerin/CMKLR1 axis represents a selective pathway natural killer (NK) cells lung. C-C motif chemokine receptor-like 2 (CCRL2) is nonsignaling seven-transmembrane domain receptor able control lung tumor growth. CCRL2 constitutive or conditional endothelial cell targeted ablation, deletion its ligand chemerin, were found promote progression in Kras/p53Flox cancer model. This...
Plasmacytoid dendritic cells (pDCs) are the major producers of type I interferons (IFNs), which essential to mount antiviral and antitumoral immune responses. To avoid exaggerated levels IFNs, pave way dysregulation autoimmunity, pDC activation is strictly regulated by a variety inhibitory receptors (IRs). In tumors, pDCs display an exhausted phenotype correlate with unfavorable prognosis, largely depends on accumulation immunosuppressive cytokines oncometabolites. This review explores...
HIV-1 matrix protein p17 activates a variety of cell responses which play critical role in viral replication and infection. Its activity depends on the expression receptors (p17R) surface target cells. Whether also plays stimulating human monocytes, major reservoir, is not known. Here we show that monocytes constitutively express p17Rs selectively triggers these cells to produce MCP-1. The effect MCP-1 was observed at transcriptional level primarily dependent activation transcription factor...
Alpha-1 antitrypsin is the main inhibitor of neutrophil elastase in lung. Although it principally synthesized by hepatocytes, alpha-1 also secreted bronchial epithelial cells. Gene mutations can lead to deficiency, with Z variant being most clinically relevant due its propensity polymerize. The ability cells produce Z-variant protein and polymers unknown. We investigated expression, accumulation, secretion Z-alpha-1 cultures transfected originating from antitrypsin-deficient patients....
C-C chemokine receptor-like 2 (CCRL2) is a non-signaling seven-transmembrane domain (7-TMD) receptor related to the atypical (ACKR) family. ACKRs bind chemokines but do not activate G protein-dependent signaling or cell functions. were shown regulate immune functions in vivo by their ability scavenge from local environment. This study was performed investigate whether CCRL2 shares two of main characteristics ACKRs, namely internalize and ligands. Cell membrane analysis CCRL2-transfected...