A5075964309- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Cardiac Structural Anomalies and Repair
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Radiomics and Machine Learning in Medical Imaging
- Gastric Cancer Management and Outcomes
- Genetic factors in colorectal cancer
- Protein Tyrosine Phosphatases
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- Glycosylation and Glycoproteins Research
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Renin-Angiotensin System Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer Immunotherapy and Biomarkers
- Renal cell carcinoma treatment
- Marine Biology and Environmental Chemistry
- Mycobacterium research and diagnosis
Shanghai Sixth People's Hospital
2024
Shanghai Jiao Tong University
2007-2022
Tongren Hospital
2020-2022
Shanghai Institute of Hematology
2018-2021
State Key Laboratory of Oncogene and Related Genes
2016-2021
Ministry of Health of the Russian Federation
2018-2021
Shanghai Cancer Institute
2017-2021
Renji Hospital
2016-2021
Beijing Jiaotong University
2020
Rabin Medical Center
2008
Abstract Background Epigenetic alterations are involved in various aspects of colorectal carcinogenesis. N 6 -methyladenosine (m A) modifications RNAs emerging as a new layer epigenetic regulation. As the most abundant chemical modification eukaryotic mRNA, m A is essential for regulation mRNA stability, splicing, and translation. Alterations regulatory genes play important roles pathogenesis variety human diseases. However, whether this participates glucose metabolism cancer (CRC) remains...
Long non-coding RNAs (lncRNAs) contribute to colorectal cancer (CRC). However, the role of lncRNAs in CRC metabolism, especially glucose metabolism remains largely unknown. In this study, we identify a lncRNA, GLCC1, which is significantly upregulated under starvation cells, supporting cell survival and proliferation by enhancing glycolysis. Mechanistically, GLCC1 stabilizes c-Myc transcriptional factor from ubiquitination direct interaction with HSP90 chaperon further specifies modification...
Increasing evidence suggests long non-coding RNAs (lncRNAs) are frequently aberrantly expressed in cancers, however, few related lncRNA signatures have been established for prediction of cancer prognosis. We aimed at developing alncRNA signature to improve prognosis gastric (GC).Using a lncRNA-mining approach, we performed expression profiling large GC cohorts from Gene Expression Ominus (GEO), including GSE62254 data set (N = 300) and GSE15459 192). 24-lncRNAs that were significantly...
Abstract Long non-coding RNAs (lncRNAs) play key roles in colorectal carcinogenesis. Here, we aimed to identify the risk SNP-induced lncRNAs and investigate their First, identified rs6695584 as causative SNP 1q41 locus. The A>G mutation of created a protein-binding motif BATF, altered enhancer activity, subsequently activated lncSLCC1 expression. Further validation two independent CRC cohorts confirmed upregulation tissues, revealed that increased expression was associated with poor...
Objective: The E3 ubiquitin ligase RNF6 (RING-finger protein 6) plays a crucial role in carcinogenesis. However, the copy number and expression of were rarely reported colorectal cancer. We aimed to explore mechanical, biological, clinical cancer initiation progression.Design: analyzed from Tumorscape Cancer Genome Atlas (TCGA) datasets. Gene expressions examined by real-time PCR, Western blot, immunohistochemical staining. profiling studies performed identify pivotal genes regulated RNF6....
Current studies indicate that long non-coding RNAs (lncRNAs) are frequently aberrantly expressed in cancers and implicated with prognosis gastric cancer (GC). We intended to generate a multi-lncRNA signature improve prognostic prediction of GC. By analyzing ten paired GC adjacent normal mucosa tissues, 339 differentially lncRNAs were identified as the candidate biomarkers Then we used LASSO Cox regression method build 12-lncRNA validated it another independent GEO dataset. An innovative was...
Abstract Studies have shown that tumor microenvironment (TME) might affect drug sensitivity and the classification of colorectal cancer (CRC). Using TME-specific gene signature to identify CRC subtypes with distinctive clinical relevance has not yet been tested. A total 18 “bulk” RNA-seq datasets (total n = 2269) four single-cell were included in this study. We constructed a “Signature associated FOLFIRI resistant Microenvironment” (SFM) could discriminate both TME sensitivity. Further, SFM...
High throughput gene expression profiling has showed great promise in providing insight into molecular mechanisms. Metastasis‐related mRNA s may potentially enrich genes with the ability to predict cancer recurrence, therefore we attempted build a recurrence‐associated signature improve prognostic prediction of colorectal ( CRC ). We identified 2848 differentially expressed by analyzing tissues or without metastasis. For selection genes, LASSO Cox regression model (least absolute shrinkage...
FAM83B (family with sequence similarity 83, member B) seems to emerge as a new class of players involved in the development variety malignant tumors. Yet molecular mechanisms are not well understood. The present study is intended investigate expression and function pancreatic ductal adenocarcinoma (PDAC). In this study, we found that was significantly increased both PDAC cell lines tumor tissues. positively related advanced clinical stage poor vital status. Higher predicted shorter overall...
Abstract Genome-wide association studies (GWAS) have identified several loci harboring variants that affected the risk of colorectal cancer; however, specific mechanisms by which germline variation influenced tumorigenesis cancer (CRC) remains unrevealed. We found T>C variant rs1317082, locating at exon 1 lncRNA RP11-362K14.5 (CCSlnc362), was predicted to be a protective locus for cancer. However, role CCSlnc362 and interaction between rs1317082 in its remain unclear. Here we explored...
Abstract Genome-wide association studies (GWASs) implicate 16q22.1 locus in risk for colorectal cancer (CRC). However, the underlying oncogenic mechanisms remain unknown. Here, through comprehensive filtration, we prioritized rs7198799, a common SNP second intron of CDH1, as putative causal variant. In addition, found an CRC-risk allele C rs7198799 with elevated transcript level biological plausible candidate gene ZFP90 via expression quantitative trait loci analysis. Mechanistically,...
AIM:To study the inhibitory effect of baculovirusmediated normal epithelial cell specific-1 (NES1 ) gene therapy on gastric cancer (GC) in vitro and vivo . METHODS: We f ir s t c o n r uc e d re m b ina nt baculovirus vectors then transfected them into cells (SGC-7901).Efficiency for transfer SGC-7901 growth curves were detected by fluorescence microscopy, Western blot 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay , respectively.The therapeutic this GC was...
Although several prognostic signatures have been developed for gastric cancer (GC), the utility of these tools is limited in clinical practice due to lack validation with large and multiple independent cohorts, or a statistical test determine robustness predictive models. Here, signature was constructed using least absolute shrinkage selection operator (LASSO) Cox regression model training dataset 300 GC patients. The verified three datasets total 658 tumors across multiplatforms. A nomogram...
Background: Epithelial-Mesenchymal Transition (EMT) is a major process in the initiation of tumor metastasis, where cancer cells lose sessile epithelial potential and gain mesenchymal phenotype. Large-scale cell identity shifts are often orchestrated on an epigenetic level interplay between factors EMT progression was still largely unknown. In this study, we tried to identify candidate that involved progression. Methods: Colorectal (CRC) were transfected with arrayed shRNA library targeting...
Autophagy has been associated with tumor progression, prognosis, and treatment response. However, an autophagy-related model their clinical significance have not yet fully elucidated. In the present study, through integrative analysis of bulk RNA sequencing single-cell sequencing, risk was identified. The capable distinguishing worse prognosis patients gastric cancer (GC), which validated in TCGA two independent Gene Expression Omnibus cohorts utilizing survival analysis, also other...
Degeneration of peripheral auditory neurons constitutes one the main causes sensorineural hearing loss. Gene delivery to inner ear is central development gene therapy for impairment. Thus we investigated effectiveness baculovirus-derived vectors transduce spiral ganglion neurons. We found that baculovirus could efficiently in vitro and highest transduced cell rate be over 75%. The level transgene expression exhibited viral dose dependence was enhanced by addition butyrate. Thus, a novel...
The management of stage II colorectal cancer is still difficult. We aimed to construct a new immune cell-associated signature for prognostic evaluation and guiding chemotherapy in cancer. used the "Cell Type Identification by Estimating Relative Subsets RNA Transcripts" (CIBERSORT) method estimate fraction 22 cells analyzing bulk tumor transcriptomes LASSO Cox regression model select cells. A 12-immune cell classifier, ISCRC, was built, which could successfully discriminate high-risk...