A5100373236- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Reconstructive Facial Surgery Techniques
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Silk-based biomaterials and applications
- Nasal Surgery and Airway Studies
- Immune cells in cancer
- Bone Tissue Engineering Materials
- Functional Brain Connectivity Studies
- Cancer, Lipids, and Metabolism
- Proteoglycans and glycosaminoglycans research
- Hepatocellular Carcinoma Treatment and Prognosis
- TGF-β signaling in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Neural dynamics and brain function
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2025
Peking Union Medical College Hospital
2014-2025
Dana-Farber Cancer Institute
2012-2024
Guangxi University
2022-2024
Qinghai Normal University
2012-2024
Chongqing Three Gorges University
2024
Hunan University of Traditional Chinese Medicine
2024
Ocean University of China
2023
Weifang Medical University
2023
University of Kentucky
2023
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spread mainly through droplets or direct contact. However, the infection condition of genital system unknown. Our aim in this study was to determine if SARS-CoV-2 present vaginal fluid women with disease 2019 (COVID-19). Methods Ten confirmed severe COVID-19 pneumonia admitted Tongji Zhongfa Hospital intensive care unit from 4 February 2020 24 were included. Clinical records, laboratory results, and computed...
Abstract Despite recent advances in extrusion bioprinting of cell‐laden hydrogels, using naturally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge. Here, we address this challenge precisely designed tough hydrogel featuring a double‐network structure. The hydrogels consisted energy‐dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid...
Adipose-derived stem cells (ASCs) can be applied extensively in the clinic because they easily isolated and cause less donor-site morbidity; however, their application complicated by patient-specific factors, such as age harvest site. In this study, we systematically evaluated effects of on quantity quality human adipose-derived mesenchymal (hASCs) from excised chest subcutaneous adipose tissue investigated underlying molecular mechanism. hASCs were donors 3 different age-groups (i.e.,...
The microRNA miR-138 is dysregulated in several human cancers, but the underlying mechanism remains largely unknown. Here, we report that commonly underexpressed nasopharyngeal carcinoma (NPC) specimens and NPC cell lines. ectopic expression of dramatically suppressed proliferation colony formation vitro inhibited tumorigenesis vivo. Moreover, identified cyclin D1 (CCND1) gene as a novel direct target miR-138. In consistent with knocked-down CCND1, overexpression growth cycle progression...
TGFβR1 plays an important role in TGF-β signaling transduction and serves as a tumor suppressor. Our previous studies show that reduced expression of is common non-small cell lung cancer (NSCLC) variants confer risk NSCLC. However, the epigenetic mechanisms underlying NSCLC carcinogenesis are still elusive. We investigated function regulation signaling-based miRNAs Computational algorithms predicted 3′-untranslated region (3′-UTR) target miR-142-3p. Here luciferase reporter assay confirmed...
Certain microRNAs (miRNAs) play a key role in cancer cell chemoresistance. However, the pleiotropic functions of exosome-derived miRNAs on developing chemoresistance remain unknown. In present study, we aimed to construct potential networks miRNAs, which derived from exosome chemoresistant prostate (PCa) cells, with their known target genes using miRNA expression profiling and bioinformatic tools. Global profiles were measured by microarray. Twelve initially selected validated qRT-PCR. Known...
Abstract The regeneration of tissue-engineered cartilage in an immunocompetent environment usually fails due to severe inflammation induced by the scaffold and their degradation products. In present study, we compared tissue remodeling inflammatory responses engineered constructed with bone marrow mesenchymal stem cells (BMSCs), chondrocytes, or both group pigs. cartilage-forming capacity constructs vitro vivo was evaluated histological, biochemical, biomechanical analyses, response...
Purpose: Hypoxia-inducible factor-2α (HIF2α) is regarded as a preferential target for individualized hepatocellular carcinoma (HCC) treatment and sorafenib resistance. Our study aimed to identify the regulatory mechanisms of HIF2α activity under hypoxic conditions. We sought determine whether COX-2/PGE2 axis involved in resistance HCC cells.Experimental Design: The cell viability, migration, invasion abilities were measured analyze effects on cells. Both vitro vivo models used driver level...
Drug-induced liver and kidney damage is an emergent clinical issue that should be addressed. Rosmarinic acid (RA) has obvious anti-inflammatory antioxidant effects, so we evaluated the effects of RA pretreatment on serum tissues cisplatin (CP)-treated mice explored possible mechanisms. The results showed effectively downregulated serum, liver, levels ALT, AST, BUN, CRE inflammatory factors IL-1β, IL-6, TNF-α, simultaneously enhanced total capacity kidney. significantly reduced MPO, MDA, NO...
Background Loss of moisture is the primary cause skin ageing and dysfunction. The skin’s hydration largely depends on hyaluronan (HA) its ability to retain water. Ultraviolet (UV) irradiation, which accounts for 80% (commonly referred as photoaging), gradually disrupts balance HA metabolism, leading a reduction in levels, dehydration, and, ultimately, formation wrinkles. Methods In this study, we develop an RNAi-based strategy treat aged by modulating endogenous metabolism. Hyaluronidase 2...
We recently demonstrated that Ski is a novel wound healing-related factor promotes fibroblast proliferation and inhibits collagen secretion. Here, we show increasing local expression by gene transfer not only significantly accelerated healing relieving inflammation, accelerating re-epithelialization formation of granulation tissue, but also reduced scar decreasing production in rat dermal wounds. Similarly, ski healing, the protuberant height volume scars increased maturity hypertrophic...
Abstract The advent of mesenchymal stem cell (MSC)-based therapies has been an exciting innovation for the treatment degenerative and inflammatory diseases. However, surface markers that accurately reflect self-renewal differentiation potential MSCs their sensitivity to environmental cues remain poorly defined. Here, we studied role CD49f in bone marrow (BMSCs) mechanism by which it regulates behavior BMSCs under conditions. We found is preferentially expressed fetal cells rather than adult...
Background: We have previously shown that 15-hydroxyeicosatetraenoic acid (15-HETE) plays a critical role in pulmonary hypertension (PH)-associated vascular remodeling. However, the signaling mechanisms remain unclear. The purpose of this study was to investigate 15-lipoxygenase-2 (15-LO-2)/15-HETE-mitogen-activated protein kinases (MAPKs) pathway hypoxia-induced remodeling and underlying mechanisms. Methods: arterial wall thickness measured by hematoxylin eosin (HE) staining distal arteries...
MicroRNA-148b (miR-148b) has been detected in various types of tumors, and is generally viewed as a tumor suppressor. Our previous study found the decreased expression miR-148b human non small cell lung cancer (NSCLC) specimens lines. However, underlying mechanisms regulating progression remain unclear. Firstly animal experiments were performed to verify whether could inhibit growth. Then, studied by transfecting recombinant plasmids containing mimic or negative control (NC) (shRNA control)...
Cyanidin-3-O-glucoside (C3G) is a kind of anthocyanin which shows strong anti-inflammation, anti-tumor and anti-oxidant properties. This paper was designed to explore the potential effects C3G on diabetic retinopathy (DR). C57BL/6 mice were administrated with streptozotocin (STZ) or vehicle control for establishment models. To simulate hyperglycemia hypoxia, D-glucose (30 mM) CoCl2 (200 μm/l) utilized treat HRECs, respectively. The migration, invasion, inflammation tube formation abilities...
The goal of this study is to determine the effects Insulin-Transferrin-Selenium (ITS) on proliferation auricular chondrocytes and formation engineered cartilage in vitro. Pig monolayer chondrocyte pellets were cultured media containing 1% ITS at different concentrations fetal bovine serum (FBS, 10%, 6%, 2%, 0%), or 10% FBS alone as a control for four weeks. Parameters including cell monolayer, wet weight, collagen type I/II/X (Col I, II, X) glycosaminoglycan (GAG) expression, GAG content...
Abstract Human dermal fibrotic disease keloid has been a clinical challenge because of its tumour-like growth and the lack effective therapy. Dysregulated alternative splicing events have demonstrated in tumours fibrosis. In current study, for first time, it was that regulator polypyrimidine tract-binding protein (PTB), which plays pivotal role tumour proliferation, invasion metastasis, is overexpressed tissues fibroblasts. Additionally, TGF-β1 upregulated expressions PTB upstream regulator,...