A5108632397- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- TGF-β signaling in diseases
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Radiomics and Machine Learning in Medical Imaging
- Circular RNAs in diseases
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Kruppel-like factors research
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Cancer Diagnosis and Treatment
- DNA Repair Mechanisms
- Immune Response and Inflammation
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Metastasis and carcinoma case studies
- Bone and Dental Protein Studies
- Metabolism, Diabetes, and Cancer
- Cerebrovascular and Carotid Artery Diseases
- Fibroblast Growth Factor Research
Soochow University
2015-2024
First Affiliated Hospital of Soochow University
2019-2024
PLA Rocket Force University of Engineering
2024
Second Affiliated Hospital of Xi'an Jiaotong University
2018-2024
Wuhan University
2022-2024
Zhongnan Hospital of Wuhan University
2022-2024
China Mobile (China)
2022-2024
China University of Geosciences
2024
Chinese Academy of Geological Sciences
2024
Ministry of Natural Resources
2024
TGF-β promotes tumor invasion and metastasis through inducing epithelial-mesenchymal transition (EMT) in non-small cell lung cancer (NSCLC). Circular RNAs (circRNAs) are recognized as functional non-coding involved human cancers. However, whether how circRNAs contribute to TGF-β-induced EMT NSCLC remain vague. Here, we investigated the regulation function of RNA hsa_circ_0008305 (circPTK2) metastasis, well a link between circPTK2 transcriptional intermediary factor 1 γ (TIF1γ) NSCLC. were...
Epithelial-mesenchymal transition (EMT), a key step in the early stages of cancer metastasis, is orchestrated by several signaling pathways, including IL-6/JAK/STAT3 and TGF-β/Smad signaling. However, an association between two pathways during EMT process largely unknown. Here, we focused on lung demonstrated that TGF-β1 induced phosphorylation Smad3 (p-Smad3), upregulation Snail, fibroblast-like morphology, downregulation E-cadherin as well vimentin cell lines. SIS3 (an inhibitor Smad3)...
CD73 (ecto-5′-nucleotidase) is implicated in the development of many types cancer. inhibitors are currently being tested clinical trials for treatment Understanding molecular and cellular actions key to improving this line therapy. Quantitative real-time PCR (qRT-PCR) was used detect expression miR-30a-5p; Western blot immunohistochemical assays were investigate levels other proteins. Flow cytometry determine cell cycle stage apoptosis. CCK-8 clonogenic proliferation. Wound healing,...
Acquired epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance limits the long-term clinical efficacy of kinase-targeting drugs. Although most mechanisms acquired EGFR-TKI have been revealed, mechanism ~ 15% cases has not yet elucidated. Cell viability was analysed using Counting Kit-8 (CCK-8) assay. Proteome profiler array analysis performed to find proteins contributing resistance. Secreted OPN detected by ELISA. Immunohistochemical conducted detect expression...
AlkB homolog 5 (ALKBH5) has been revealed as a key RNA N6-methyladenosine (m6A) demethylase that is implicated in development and diseases. However, the function of ALKBH5 TGF-β-induced epithelial-mesenchymal transition (EMT) tumor metastasis non-small-cell lung cancer (NSCLC) remains unknown. Here, we firstly show expression significantly reduced metastatic NSCLC. overexpression inhibits EMT invasion NSCLC cells, whereas knockdown promotes corresponding phenotypes. suppresses...
Abstract Effective detection of bio‐molecules relies on the precise design and preparation materials, particularly in laser desorption/ionization mass spectrometry (LDI‐MS). Despite significant advancements substrate performance single‐structured substrates remains suboptimal for LDI‐MS analysis complex systems. Herein, designer Au@SiO 2 @ZrO core‐shell are developed LDI‐MS‐based early diagnosis prognosis pancreatic cancer (PC). Through controlling Au core size ZrO shell crystallization,...
Abstract Yes-associated protein (YAP) is a central player in cancer development, with functions extending beyond its recognized role cell growth regulation. Recent work has identified link between YAP/transcriptional coactivator PDZ-binding motif (TAZ) and the DNA damage response. Here, we investigated mechanistic underpinnings of cross-talk repair YAP activity. Ku70, key component nonhomologous end joining pathway to damage, engaged dynamic competition TEAD4 for binding YAP, limiting...
TGFβR1 plays an important role in TGF-β signaling transduction and serves as a tumor suppressor. Our previous studies show that reduced expression of is common non-small cell lung cancer (NSCLC) variants confer risk NSCLC. However, the epigenetic mechanisms underlying NSCLC carcinogenesis are still elusive. We investigated function regulation signaling-based miRNAs Computational algorithms predicted 3′-untranslated region (3′-UTR) target miR-142-3p. Here luciferase reporter assay confirmed...
Abstract Background Artemin (ARTN) is a neurotrophic factor belonging to the glial cell-derived family of ligands. To develop potential therapy targeting ARTN, we studied roles miR-223 in migration and invasion human esophageal carcinoma. Methods ARTN expression levels were detected carcinoma cell lines KYSE-150, KYSE-510, EC-9706, TE13, cancer tissues paired non-cancerous by Western blot. siRNA vectors constructed knockdown artemin mitigated invasiveness KYSE150 cells. Monolayer wound...
Significant evidence has shown that the miRNA pathway is an important component in downstream signaling cascades of TGF-β1 pathway. Our previous study indicated miR-335-5p expression was significantly down-regulated and acted as a vital player metastasis non-small cell lung cancer (NSCLC), however underlying mechanism remained unclear.The differential level ROCK1 were determined by qRT-PCR IHC analysis human tissue samples with or without lymph node metastasis. Transwell assay conducted to...
Article26 March 2021Open Access Source DataTransparent process Quaking 5 suppresses TGF-β-induced EMT and cell invasion in lung adenocarcinoma Shengjie Wang orcid.org/0000-0003-0320-0377 Soochow University Laboratory of Cancer Molecular Genetics, Medical College University, Suzhou, China Department School Biology Basic Sciences, Medicine, Kangda Nanjing Lianyungang, ChinaThese authors contributed equally to this work Search for more papers by author Xin Tong Chang Li Thoracic Surgery, The...
Abstract Regulator of G-protein signaling 6 (RGS6) is a newly discovered tumor suppressor that has been shown to be protective in development various cancers such as breast cancer and bladder cancer. But the mechanisms underlying these tumor-suppressing functions RGS6 are not fully understood. Here, we discover novel function suppressing TGF-β-induced epithelial–mesenchymal transition (EMT) non-small cell lung (NSCLC) cells vivo NSCLC metastasis. Using both bioinformatics experimental tools,...
The TGF-β/Smad signaling pathway plays important roles in cancer cell proliferation, apoptosis, differentiation, angiogenesis and epithelial-mesenchymal transition (EMT), which is the key event early stages of metastasis enhances capability migration invasion. Smad4 acts as only Co-Smad TGF/Smad role TGF-β-mediated EMT. Nevertheless, mRNA regulation mechanisms human non-small lung (NSCLC) remains largely unclear. Computational algorithms predicted that 3'-UTR a target miR‑205. Here, we...
// Xiangguang Shi 1,2,* , Lei Zhan Can Xiao 3,* Zhe 1,2 Haiping Yang Longqiang Wang Jun Zhao 2,3 and Hong-Tao Zhang 1 Soochow University Laboratory of Cancer Molecular Genetics, Medical College University, Suzhou, China 2 Suzhou Key for 3 The First Affiliated Hospital * These authors have contributed equally to this work Correspondence to: Zhang, email: Keywords : NSCLC, miR-1238, LHX2, cell proliferation Received January 30, 2015 Accepted May 13, Published 22, Abstract In human cancers,...