Circulating exosomes in bodily fluids such as blood are being actively studied a rich source of chemical biomarkers for cancer diagnosis and monitoring. Although nucleic acid analysis is primary tool the discovery circulating exosomes, metabolomics holds potential expanding diversity that may be easy rapid to detect. However, only trace amounts can isolated from small volume patient blood, thus very sensitive technique required analyze metabolome exosomes. In this report, we present workflow...

A key step in metabolomics is to perform accurate relative quantification of the metabolomes comparative samples with high coverage. Hydroxyl-containing metabolites are an important class metabolome diverse structures and physical/chemical properties; however, many them difficult detect sensitivity. We present a high-performance chemical isotope labeling liquid chromatography mass spectrometry (LC-MS) technique for in-depth profiling hydroxyl submetabolome, which involves use acidic...

In cellular metabolomics, it is desirable to carry out metabolomic profiling using a small number of cells in order save time and cost. some applications (e.g., working with circulating tumor blood), only limited are available for analysis. this report, we describe method based on high-performance chemical isotope labeling (CIL) nanoflow liquid chromatography mass spectrometry (nanoLC-MS) high-coverage analysis numbers (i.e., ≤10000 cells). As an example, 12C-/13C-dansyl the metabolites...

Purpose To prospectively assess the feasibility of using virtual iron content (VIC) imaging at dual-energy computed tomography (CT) to evaluate liver (LIC) in patients suspected having overload and compare LIC grading performance VIC magnetic resonance (MR) imaging. Materials Methods This study was approved by institutional review board, informed consent obtained from all patients. Fifty-six (serum ferritin concentrations >500 μg/L) underwent unenhanced CT MR liver. imaging–measured LICs...

Plums are widely consumed, contain high levels of phenolic compounds, and have strong antioxidant activity. In this study, the main Sichuan cultivars 'Qiangcuili' 'Cuihongli' were used to study changes in appearance, internal quality, activities during fruit development expression phenolic-compound-related structural genes. The results showed that, two plums, total soluble solid sugar contents highest at mature stage. (total phenol content (TPC), flavonoid (TFC), flavanol (TFAC)) trended...

Abstract The most commercialized kiwifruit, Actinidia chinensis var. deliciosa ( Acd ), is an allohexaploid (2n = 6x 174), making high-quality assemblage genome challenging. We previously discovered a rare naturally occurring diploid plant. Here, chromosome-level de novo assembly for this was reported, reaching approximately 621.98 Mb in length with contig and scaffold N50 values of 10.08 21.09 Mb, respectively, 99.66% the bases anchored to 29 pseudochromosomes, 38,990 protein-coding genes...

Cadmium (Cd) contamination of paddy soil affects safe crop production. This study aimed to evaluate the effects plant biostimulant amino acid fertilizer on phytoremediation capability an emergent accumulator Nasturtium officinale R. Br. for Cd-contaminated soils. A pot was carried out different concentrations Cd accumulation N. grown in soil. The increased biomass officinale. concentration exhibited a quadratic polynomial regression relationship with root and shoot biomass. also...

To elucidate the biochemical mechanisms underlying Rlm1-mediated blackleg resistance, we performed a metabolomic analysis comparing susceptible DH line of Topas and its isogenic Rlm1-carrying resistant counterpart. Our results revealed significant differences in metabolite profiles post-inoculation. resistance was characterized by early sustained upregulation lysine degradation metabolites, particularly pipecolic acid (PA), which increased up to 326-fold, highlighting role resistance....

The dairy industry relies on the health and well-being of cows for optimal production milk products. Mastitis, a prevalent economically burdensome disease characterized by udder inflammation, poses significant challenges to farmers worldwide. In this study, we employed peptidomics explore peptide profiles dry secretions collected from at specific intervals during dry-off period. We hypothesized that alterations in composition period may influence pathogen proliferation immune cell...

Pomegranate (Punica granatum L.), with its abundant phenolic substances and strong antioxidant activity, holds significant research utilization potential across various organs. However, there have been few studies on the content activity of different parts pomegranate, especially placenta. This study investigated fruits, flowers, leaves two pomegranate varieties, 'Tunisia' 'Qingpi', throughout their growth development. Results indicated variations in among organs, petals exhibiting highest...

This work focused on the metabolomic profiling of conditioned medium (FS03CM) produced by an anaerobic bacterium closely related to Terrisporobacter spp. identify potential antimicrobial metabolites. The metabolome was profiled two-channel Chemical Isotope Labelling (CIL) LC-MS. detected metabolites were identified or matched conducting a library search using different confidence levels. Forty-eight significantly changed with high after growth isolate FS03 in cooked meat glucose starch...

Information about how yeast metabolism is rewired in response to internal and external cues can inform the development of metabolic engineering strategies for food, fuel, chemical production this organism. We report a new metabolomics workflow characterization such rewiring. The combines efficient cell lysis without using chemicals that may interfere with downstream sample analysis differential isotope labeling liquid chromatography mass spectrometry (CIL LC–MS) in-depth metabolome...

Bi-allelic mutations in the dedicator of cytokinesis 8 (DOCK8) are responsible for a rare autosomal recessive primary combined immunodeficiency syndrome, characterized by atopic dermatitis, elevated serum Immunoglobulin E (IgE) levels, recurrent severe cutaneous viral infections, autoimmunity, and predisposition to malignancy. The molecular link between DOCK8 deficiency skin inflammation remains unknown. Severe dermatitis (AD) share some clinical symptoms, including eczema, eosinophilia,...

The selenium (Se) deficiency is threatening the human health, and increase of Se content in food can prevent body. To fruit trees alleviate stress to trees, effects gibberellic acid (GA) on growth accumulation Cyphomandra betacea under were studied. Although GA increased biomass C. betacea, it did not significantly affect root/shoot ratio. root shoot had a quadratic polynomial regression relationship with concentration. Furthermore, photosynthetic pigment content, parameters, antioxidant...

Abstract Background Oral melanoma (OM) and oral squamous cell carcinoma (OSCC) are frequently diagnosed in dogs, presenting a challenge distinguishing them from benign tumors (BN). Salivary metabolomic biomarkers offer practical solution because of saliva's direct contact with the noninvasive nature collection. Objective Assess diversity abundance salivary metabolome dogs BN, OM, OSCC using amine/phenol submetabolome analysis high‐performance chemical isotope labeling liquid...

Jujube is a characteristic light-demanding fruit tree, but quality frequently degraded because of the weak light in protected cultivation facilities south China. In this study, black shade net was used to set three intensities artificially: no shading (0%, control, NS), moderate (45%, MS), and severe (76%, SS) investigate effect low on sugar metabolism jujube. Compared with NS, chlorophyll carotenoid contents shaded leaves were increased, while specific leaf weight decreased. A decrease...

Pre-existing and gestationally-developed diabetes mellitus have been linked with impairments in placental villous trophoblast cell metabolic function, that are thought to underlie the development of diseases early lives exposed offspring. Previous research using lines ex vivo preparations highlighted hyperglycemia is an important independent regulator function. However, it poorly understood if directly influences aspects including nutrient storage mitochondrial respiration, altered term...

Plums are good sources of various bioactive phytochemical compounds such as vitamins, anthocyanins, and carotenoids, whereby all which noted for multiple potential health benefits. However, knowledge regarding plum carotenoid profiles remains limited. Hence, the total individual carotenoids in edible parts (skin flesh) ten cultivars were determined using a spectrophotometer high-performance liquid chromatography-diode array detection, respectively. Total contents skin significantly higher (

The placenta resides in a physiologically low oxygen microenvironment of the body. Hypoxia induces wide range stem cell cellular activities. Here, we report workflow for exploring role physiological (hypoxic, 5% oxygen) and original culture (normoxic, 21% concentrations regulating metabolic status human placenta-derived mesenchymal cells (hPMSCs). general biological characteristics hPMSCs were assessed via variety approaches such as counts, flow cytometry differentiation study. A sensitive...

Background: Glucagon-like peptide-1 receptor (GLP-1R) agonists are able to inhibit bone resorption a certain extent and improve formation. GLP-1R single nucleotide polymorphism (SNP) is related its activity, but the relationship between SNP osteoporosis in postmenopausal women was still unclear. This study investigate association mineral density (BMD) Shanghai. Methods: Eight SNPs of were detected (rs3765467, rs1042044, rs2268657, rs6923761, rs2268641, rs2295006, rs4714210 rs10305420) 884...