A5101601814- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Pharmacological Effects and Assays
- Cancer therapeutics and mechanisms
- Receptor Mechanisms and Signaling
- Phytochemistry and Biological Activities
- Synthesis and Biological Evaluation
- Flavonoids in Medical Research
- Cardiovascular Effects of Exercise
- Natural product bioactivities and synthesis
- Metabolism, Diabetes, and Cancer
- Macrophage Migration Inhibitory Factor
- Plant tissue culture and regeneration
- Bioactive natural compounds
- Autophagy in Disease and Therapy
- Ferroptosis and cancer prognosis
- Lung Cancer Research Studies
- Toxin Mechanisms and Immunotoxins
- Liver Disease Diagnosis and Treatment
- Cardiovascular and exercise physiology
- Essential Oils and Antimicrobial Activity
Affiliated Hospital of Hangzhou Normal University
2024-2025
Renji Hospital
2024
Hangzhou Normal University
2024
Feed Research Institute
2024
Chinese Academy of Agricultural Sciences
2024
Shanghai Jiao Tong University
2024
Zhejiang University
2018-2021
First Affiliated Hospital Zhejiang University
2018-2021
Second Military Medical University
2021
Shihezi University
2021
Abstract The present study was carried out to investigate the lipid‐lowering effect of luteolin by using a cell model steatosis induced palmitate. Incubation HepG2 cells with palmitate markedly increased lipid accumulation (Oil Red O staining), genes involved in lipogenesis, including fatty acid synthase (FAS) and its upstream regulator sterol regulatory element binding protein 1c (SREBP‐1c), reactive oxygen species (ROS) production. Luteolin enhanced phosphorylation AMP‐activated kinase α...
β-transducin repeat-containing protein (β-TrCP), one of the best-characterized substrate recognition components SKP1-CUL1-F-box (SCF) E3 ligase, has two distinct paralogs, β-TrCP1 and β-TrCP2, expressed in mammals. Through governing ubiquitination degradation numerous key regulators, β-TrCP1/2 regulates various cellular physiological pathological processes. However, whether how these proteins cross talk they regulate cell autophagy proliferation different manners is completely unknown....
Isatis indigotica (Ii) is a cruciferous herb that widely distributed in China, and its roots leaves have been used two renowned antipyretic detoxicate crude drugs Chinese Pharmacopoeia, Radix (R) Folium (F) Isatidis. However, the pharmacodynamic material basis underlying mechanisms of herbal efficacy remained to be elucidated.Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was adopted for chemical profiling R F The active ingredients were...
AMP-activated protein kinase (AMPK) plays an important role in mediating energy metabolism and is controlled mainly by two upstream kinases, LKB1 or Ca2+/calmodulin-dependent kinase-β (CaMKKβ). Previously, we found that baicalin, one of the major flavonoids a traditional Chinese herb medicine, Scutellaria baicalensis, protects against development hepatic steatosis rats feeding with high-fat diet activation AMPK, but, underlying mechanism for AMPK unknown. Here show LKB1-deficient cells, HeLa...
The ATR/CHK1 pathway is a key effector of cellular response to DNA damage and therefore critical regulator genomic stability. While the often inactivated by mutations, CHK1 itself rarely mutated in human cancers. Thus, levels likely play role maintenance stability preventing tumorigenesis. Glucose deprivation observed many solid tumors due high glycolytic rates cancer cells insufficient vascularization, yet have devised mechanisms survive conditions low glucose. Although degradation through...
Abstract Topoisomerase II (TOP2)-targeting anticancer chemotherapeutic drugs, termed TOP2 poisons, are widely used and effective in the clinic by stabilizing TOP2-DNA covalent complexes to induce DNA double-strand breaks (DSBs) ultimately, cause cell death. The stabilized complex is known be degraded proteasome, whereas underlying mechanism for instant TOP2β degradation response poisons subsequent biological consequence remain elusive. Here, we reported that poison-induced mediated SCF...
Our previous studies have demonstrated that Mito-Tempol (also known as 4-hydroxy-Tempo), a mitochondrial reactive oxygen species scavenger, alleviates oxidized low-density lipoprotein (ox-LDL)-triggered foam cell formation. Given the effect of oxidative stress on activating NOD-, LRR-, and pyrin domain-containing 3 (NLRP3) inflammasome, which promotes formation, we aimed to explore whether Mito-Tempo inhibits ox-LDL-triggered formation by regulating NLRP3 inflammasome. The results revealed...
Physiologically Based Pharmacokinetic (PBPK) models can provide forecasts of the drug residues within organism. Ractopamine (RAC) is a typical β-agonist. In this study, we developed PBPK model for RAC in goats. The goal was to predict distribution after multiple oral administrations. preliminary goats performed well predicting drug’s most tissues. our sensitivity analysis, found that parameter Qclu (Blood Flow Volume through Lungs) had greatest impact on concentrations plasma, liver, and...
Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been identified as a regulatory molecule in angiogenesis. The goal of this study was to illustrate how MEG3 affects the angiogenesis vascular endothelial cells. Expression MEG3, miR-147 and intracellular cell adhesion molecule-1 (ICAM-1) human microvascular line (HMEC-1) altered by transfection, then viability, apoptosis, migration, tube formation, well correlation among ICAM-1 were explored. down-regulated during formation...
Abstract Differences between isogenic mouse strains in cellular expression of the neuronal nicotinic acetylcholine (ACh) receptor subunit alpha4 (nAChRα4) by dorsal hippocampus are well known. To investigate further genetic basis these variations, nAChRα4 was measured congenic lines derived from two exhibiting notable divergence this subunit: C3H and C57BL/6. Congenic carrying reciprocally introgressed regions (quantitative trait loci; QTL) chromosomes 4, 5, 12 each retained phenotype most...
Congenic mapping is a powerful strategy to identify genomic loci regulating quantitative traits. Generating congenic lines an iterative process of refinement that both time and resource intensive. Here we report alternative traditional microsatellite marker analysis or costly high-density oligonucleotide single nucleotide polymorphism (SNP) arrays for genotyping. The identification inherited SNP variability in using high resolution melting (HRMA) represents novel application the method....
In solid tumors, cancer cells have devised multiple approaches to survival and proliferate in response glucose starvation that is often observed tumor microenvironments. However, the precise mechanisms are far less known. Herein, we report deprivation activates 90-kDa ribosomal S6 kinase (p90 RSK), a highly conserved Ser/Thr kinase, activated p90 RSK promotes cell survival. Mechanistically, by phosphorylates checkpoint 1 (CHK1), key transducer signaling pathways, at Ser280 triggers CHK1...
For non-professionally trained athletes, the hyperphysiological stress response induced by marathon exercise may lead to heart fatigue and even cause organic changes such as abnormal ventricular septal thickening. Early detection of cardiac function in athletes can help prevent adverse cardiovascular events. The purpose this study was analyze left systolic (LVSF) amateur runners using a novel technique called (LV) pressure-strain loop (PSL).
Aronia melanocarpa berries and their extracts, have become well known for notable health benefits.The present study investigated the anti-inflammatory effect of anthocyanin-rich phenolic extracts Ame (Aronia melanocarpa) in LPS (lipopolysaccharide)-stimulated RAW 264.7 murine macrophage cells.The results showed that pre-treatment significantly ameliorated oxidative stress inflammatory biomarker activities, as evidenced by reductions production ROS (reactive oxygen specie), MDA...
Objective To screen out the plasma miRNA which may be differentially expressed in colorectal cancer.Methods Differential expression of was detected by using TaqMan(R)MicroRNA Array from 7 patients with cancer and 6 healthy volunteers.The results were validated samples 29 20 volunteers real-time polymerase chain reaction (Real-time PCR).Results Sixteen plasma-specific miRNAs identified cancer,miR-125b,miR-375,miR-150 miR-206 PCR consistent TaqMan(R) MicroRNA had statistical significant...
Abstract Lyme Disease is caused by infection with the spirochete Borrelia burgdorferi, and most common vector-borne disease in United States. Often, leads to acute arthritis humans. Our lab others have exploited mouse model system elucidate mechanical mechanisms of development. Because miRNAs been shown be important regulators inflammatory immune responses, we performed a microarray screen for expression joint tissue from three infected strains. This identified upregulation miR-146a, key...