A5018233043- Rheumatoid Arthritis Research and Therapies
- Cytokine Signaling Pathways and Interactions
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Autoimmune and Inflammatory Disorders Research
- Monoclonal and Polyclonal Antibodies Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- SARS-CoV-2 and COVID-19 Research
- Signaling Pathways in Disease
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Chemokine receptors and signaling
- Bone Metabolism and Diseases
- Vasculitis and related conditions
- Adipokines, Inflammation, and Metabolic Diseases
- Systemic Sclerosis and Related Diseases
- Salivary Gland Disorders and Functions
- Immune cells in cancer
- T-cell and B-cell Immunology
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Immunodeficiency and Autoimmune Disorders
- Intramuscular injections and effects
- Peptidase Inhibition and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biosimilars and Bioanalytical Methods
Hospital Universitario de La Princesa
2019-2024
Universidad Autónoma de Madrid
2020-2021
Abstract B and T cell responses were evaluated in patients with rheumatoid arthritis (RA) or psoriatic (PsA) after 1 2 weeks of methotrexate (MTX) withdrawal following each COVID-19 vaccine dose compared those who maintained MTX. Adult RA PsA treated MTX recruited randomly assigned to 3 groups: MTX-maintenance ( n = 72), MTX-withdrawal for week 71) 73). Specific antibodies several SARS-CoV-2 antigens interferon (IFN)-γ interleukin (IL)-21 assessed. without previous was associated higher...
Rheumatoid Arthritis-associated Interstitial Lung Disease (RA-ILD) significantly reduces life quality and survival, necessitating improvements in its understanding clinical management. We addressed these goals using DNA methylation analysis, which has not been done RA-ILD samples, by comparing 32 RA patients with ILD diagnosed less than one year before (cases) matched without (controls). This analysis identified 6679 differentially methylated positions (DMPs) Δβ ≥ 2% FDR < 0.05, 576 regions...
Primary Sjögren's syndrome (pSS) is an inflammatory autoimmune disorder largely mediated by type I and II interferon (IFN). The potential contribution of innate immune cells, such as natural killer (NK) cells dendritic (DC), to the pSS pathology remains understudied. Here, we identified enriched CD16+ CD56hi NK cell subset associated with higher cytotoxic function, well elevated proportions CD64+ conventional (cDC2) subtype that expresses increased levels MICa/b, ligand for activating...
The individual contribution of specific myeloid subsets such as CD1c+ conventional DC (cDC) to perpetuation rheumatoid arthritis (RA) pathology remains unclear. In addition, the innate sensors driving pathogenic activation cDC in patients with RA and their functional implications have not been characterized. Here, we assessed phenotypical, transcriptional, characteristics CD141+ monocytes from blood synovial fluid RA. Increased levels CCR2 IgG receptor CD64 on circulating was associated...
The aim of this study was to identify single-nucleotide polymorphisms (SNPs) in bone remodeling-related genes associated with disease severity and mineral density (BMD) early arthritis (EA) patients. For purpose, the genotyping 552 SNPs located gene regions semaphorins 4b, 4d, 4f, DKK1, 2 3, sclerostin, OPG, RANK RANKL performed using Immunochip from Illumina Inc. 268 patients Princesa Early Arthritis Register Longitudinal (PEARL) study. Measurements BMD activity were chosen as outcome...
Galectin 1 (Gal1) is a lectin with wide cellular expression that functions as negative regulator of the immune system in several animal models autoimmune diseases. Identification patients rheumatoid arthritis (RA) has improved during last decade, although there still need for biomarkers allowing an early diagnosis. In this regard, it been recently proposed Gal1 serum levels are increased RA compared to general population. However, topic controversial literature. work, we provide additional...
The identification of “trained immunity/tolerance” in myeloid cells has changed our perception the performance monocytes and macrophages during inflammatory immune responses. Pemetrexed (PMX) methotrexate (MTX) are blockers one-carbon metabolism (OCM) commonly used therapeutic agents cancer rheumatoid arthritis (RA). We have previously showed that MTX promotes trained immunity human macrophages. In present manuscript, we assessed anti-inflammatory effects PMX found OCM alter functional gene...
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by an autoimmune response in the joints and exacerbation of cytokine responses. A minority patients with RA experience spontaneous remission, but most will show moderate/high activity, aggressive joint damage multiple systemic manifestations. There thus great need to identify prognostic biomarkers for risk improve diagnosis prognosis, inform on appropriate therapy. Here we focused suppressor signaling 1 (SOCS1),...
Abstract The presence of rheumatoid factor (RF) or anti-cyclic citrullinated peptide (anti-CCP) autoantibodies contributes to the current arthritis (RA) classification criteria. These criteria involve stratification on antibody levels, which limits reproducibility, and underperform in RA patients without RF anti-CCP. Here, we have explored if two anti-acetylated antibodies (AAPA), lysine (AcLys) ornithine (AcOrn), could improve performance analysis was done 1062 prospectively-followed early...
Few studies have considered immune-mediated inflammatory disorders (IMID) together, which is necessary to adequately understand them given they share common mechanisms. Our goal was investigate the expression of vasoactive intestinal peptide (VIP) and its receptors VPAC1 VPAC2 in selected IMID, analyze effect biological therapies on them, identify miRNA signatures associated with their expression. Serum VIP levels mRNA VPAC peripheral blood mononuclear cells were analyzed from 52 patients...
Galectin-1 (Gal1) plays a regulatory role in the immune system. We have recently validated that Gal1 serum (sGal1) levels are increased rheumatoid arthritis (RA) patients compared to healthy donors (HDs); however, there is no information on spondyloarthritis (SpA). Objective: To compare with SpA versus RA as diagnostic biomarker. Methods: studied sGal1 HD (n = 52), 80) and 64) who were randomly divided into discovery validation sets. Synovial fluid (SF) from osteoarthritis (OA) 28),...
Pro-inflammatory CD4+CD28− T cells are characteristic of immunosenescence, but also several autoimmune/inflammatory diseases. Vasoactive intestinal peptide (VIP) acts as an anti-inflammatory and immunomodulatory mediator on these cells. Our objective was to study the mutual influence between senescent Th VIP axis in early arthritis (EA), comparing with non-EA donors. We characterized correlation clinic parameters EA well behavior biomarkers by real-time PCR. Clinical data were systematically...
Objective: To compare the capacity of various disease activity indices to evaluate changes in function, IL-6 levels, and radiographic progression early established rheumatoid arthritis (RA). Methods: Secondary data analysis a clinical trial assessing efficacy tocilizumab patients with RA (ACT-RAY) longitudinal prospective register (PEARL). Targeted outcomes were physical measured health assessment questionnaire (HAQ), serum progression. The “Hospital Universitario La Princesa Index” (HUPI),...
Galectin 1 (Gal1) exerts immunomodulatory effects leading to therapeutic in autoimmune animal models. Patients with rheumatoid arthritis have been reported show higher Gal1 serum levels than the healthy population. Our study aimed find genetic variants on gene (LGALS1) modulating its expression and/or clinical features patients early (EA). LGALS1 was sequenced 53 EA characterize all variants. Then, we genotyped rs9622682, rs929039, and rs4820293, which covered main variation LGALS1, 532...
<h3>Background:</h3> At the beginning of COVID-19 pandemic there were controversial opinions regarding possibility temporary interrupting immunomodulatory treatments in rheumatic patients to enhance immune response vaccine. Previous published works have raised concern that methotrexate (MTX) might a negative effect on upon vaccine administration this population. In context, we postulated MTX interruption after could be beneficial for rheumatoid arthritis (RA) and psoriatic (PsA) terms...
Abstract The initial management of rheumatoid arthritis (RA) has a high impact on disease prognosis. Therefore, we need to select the most appropriate treatment as soon possible. This goal requires biomarkers severity and One such biomarker may be presence anti-carbamylated protein antibodies (ACarPA) because it is associated with adverse long term outcomes radiographic damage mortality. Here, have assessed ACarPA short-term prognostic biomarkers. study was conducted in 978 prospective early...
CD40, BLK and BANK1 genes involved in the development signaling of B-cells are identified as susceptibility loci for numerous inflammatory diseases. Accordingly, we assessed potential influence on pathogenesis immunoglobulin-A vasculitis (IgAV), predominantly a B-lymphocyte condition. Three genetic variants within CD40 (rs1883832, rs1535045, rs4813003) (rs2254546, rs2736340, rs2618476) well two polymorphisms (rs10516487, rs3733197), previously associated with diseases, were genotyped 382...