Inflammatory processes induced by leukocytes are crucially involved in the pathophysiology of acute ischemic stroke. This study aimed to elucidate inflammatory mechanism long non-coding RNA (lncRNA) H19-mediated regulation C1q and tumor necrosis factor 6 (C1QTNF6) sponging miR-29b during stroke.H19 expression patients with stroke rats middle cerebral artery occlusion were measured real-time polymerase chain reaction. H19 siRNA antagomir used knock down miR-29b, respectively. We performed...

Background: Vitamin D (VD) deficiency has become a global public health problem, and published studies have demonstrated that patients with subacute thyroiditis (SAT) worse VD nutritional status supplementation may alleviate thyroid-related diseases by fighting against infections mediating autoimmunity. Objectives: This study explored the correlation between serum levels risk extent of disease in SAT. Design: A case-control study. Methods: We included SAT diagnosed at First People’s Hospital...

Hjm and Hel308a are novel, RecQ-like DNA helicases recently identified in the euryarchaeotes Pyrococcus furiosus Methanothermobacter thermautotrophicus, respectively. In this study, an Hjm/Hel308 homologue (designated StoHjm) from Sulfolobus tokodaii, a hyperthermophilic archaeon belonging to Crenarchaeota subdomain of archaea, was cloned, purified, characterized. Unlike Hel308a, which unwind 3'-to-5' direction, StoHjm unwound both 5'-to-3' directions. Remarkably, exhibited...

In this study, the regulator MalR (Saci_1161) of TrmB family from Sulfolobus acidocaldarius was identified and shown to be involved in transcriptional control maltose regulon (Saci_1660 Saci_1666), including ABC transporter (malEFGK), α-amylase (amyA), α-glycosidase (malA). The ΔmalR deletion mutant exhibited a significantly decreased growth rate on dextrin but not sucrose. expression genes organized induced only presence medium, indicating that MalR, contrast its TrmB-like homologues, is an...

Alendronate is commonly used for the treatment of postmenopausal osteoporosis; however, underlying pathological molecular mechanisms its action remain unclear. In present study, alendronate-treated signaling pathway in bone metabolism rats with ovariectomy induced by osteoporosis was investigated. Rats were orally administered alendronate or phosphate-buffered saline (control). addition, interferon-β (IFN-β)/signal transducer and activator transcription 1 (STAT1) investigated osteoblasts...

Following cerebral ischemia, microRNA- (miR-) 29b in circulating blood is downregulated. This study investigates the underlying mechanism and implications of miR-29b leukocyte induction.miR-29b from stroke patients rats with middle artery occlusion (MCAO) were assessed using real-time polymerase chain reaction (PCR). agomir was used to increase expression leukocytes via intravenous injection. C1q tumor necrosis factor (C1QTNF) 6, interleukin- (IL-) 1β, zonula occludens- (ZO-) 1, occludin,...

The ups operon of Sulfolobus species is highly induced upon UV stress. Previous studies showed that the pili encoded by this are involved in cellular aggregation, which essential for subsequent DNA exchange between cells, resulting homologous recombination. presence pilus system increases fitness cells under light-induced stress conditions, as transfer takes place order to repair UV-induced lesions via Four conserved genes (saci_1497 saci_1500) encode proteins with putative processing...

Abstract Background Disulfidptosis and Ferroptosis are two novel forms of cell death. Although their mechanisms differ, research has shown that there is a relationship between the two. Investigating connection these death can further deepen our understanding development progression cancer, provide better prediction models for accurate prognosis. Methods In this study, RNA sequencing (RNA-seq) data, clinical single nucleotide polymorphism (SNP) single-cell data were obtained from public...

To explore facilitators and barriers to self-management engagement of Chinese people with poorly controlled type 2 diabetes.Purposive sampling method was used for recruitment. Semi-structured interview thematic analysis data collection analysis.Twenty-six semi-structured interviews were conducted. Poor blood glucose control introduced awareness susceptibility complications, while mental disorders could be concomitant. General knowledge about healthy lifestyle unhealthy habits impeded...

Pharmacognosy Magazine,2012,8,32,300-307.DOI:10.4103/0973-1296.103659Published:November 2012Type:Original ArticleAuthors:Hao Cai, Zhiwei Xu, Sucai Luo, Wenwen Zhang, Gang Cao, Xiao Liu, Yajing Lou, Xiaoqing Ma, Kunming Qin, and Baochang Cai Author(s) affiliations:Hao Cai1, Xu2, Luo2, Zhang2, Cao2, Liu1, Lou3, Ma3, Qin3, Cai4 1Department of Chinese Materia Medica, College Pharmacy, Nanjing University Medicine, - 210046; Engineering Center State Ministry Education for Standardization Medicine...

To investigate the neuroprotective effect and mechanism of cerebroprotein hydrolysate-I (CH-I) on cerebral ischemia/reperfusion injury in rats.A total 100 adult healthy male SD rats were randomly divided into a sham group, model CH-I treated cerebrolysin (CBL) positive consisting 20 each group. The middle artery occlusion/reperfusion (MCAO/R) was built by inserting suture left external carotid (ECA) through internal (ICA). Treatment performed intraperitoneal injection (20 mg/kg)....

Purpose. We investigated whether a DDP-4 inhibitor, vildagliptin, alleviated ER stress induced by high fat diet and improved hepatic lipid deposition. Methods. C57BL/6 mice received standard chow (CD), (HFD), HFD administered with vildagliptin (50 mg/Kg) (V-HFD). After administration for 12 weeks, serum alanine aminotransferase, glucose, cholesterol, triglyceride, insulin levels were analyzed. Samples of liver underwent histological examination transmission electron microscopy, real-time PCR...

Abstract Background Lung cancer has the highest morbidity and mortality in world novel treatment strategies are still needed. Haimufang decoction (HMF) is a patented clinical prescription of traditional Chinese medicine for lung treatment. HMF composed four herbs been applied clinically advanced patients. However, its therapeutic mechanisms unclear. This study aims to elucidate possible cancer. Methods 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was evaluating...

A well-developed root system is crucial for the rapid growth, asexual reproduction, and adaptation to drought environments of watercress. After analyzing transcriptome watercress system, we found that a high concentration auxin key its dry conditions. For first time, obtained DR5::EGFP watercress, which revealed dynamic distribution in development under Via application naphthylphthalamic acid (NPA), 4-biphenylboronic (BBO), ethylene (ETH), abscisic (ABA), other factors, confirmed has...

Breast cancer remains the leading malignant neoplasm among women globally, posing significant challenges in terms of treatment and prognostic evaluation. The metabolic pathway polyamines is crucial breast progression, with a strong association to increased capabilities tumor cells for proliferation, invasion, metastasis. We used multi-omics approach combining bulk RNA sequencing single-cell (scRNA-seq) study polyamine metabolism. Data from Cancer Genome Atlas, Gene Expression Omnibus,...

Alcoholism can lead to low mineral density, compromised regenerative bone capacity and delayed osteointegration of dental implants. This may be partially attributed the inhibitive effect all-trans retinoic acid (ATRA), a metabolite alcohol, on osteoblastogenesis. Our previous studies demonstrated that heterodimeric morphogenetic protein 2/7 (BMP2/7) was more potent BMP than homodimeric BMP2 or BMP7, could antagonize ATRA rescue osteoblastogenesis.In this study, we compared effectiveness...