To survive, plants must respond rapidly and effectively to various stress factors, including biotic abiotic stresses. Salinity triggers the increase of cytosolic free Ca2+ concentration ([Ca2+]i) via influx across plasma membrane, as well bacterial flg22 plant endogenous peptide Pep1. However, interaction between stress-induced [Ca2+]i increases is still not clear. Employing an aequorin-based imaging assay, in this work, we investigated changes response flg22, Pep1, NaCl treatments...

Pyroptosis was recently demonstrated to be an inflammatory form of gasdermin-regulated programmed cell death characterized by cellular lysis and the release several proinflammatory factors participates in tumorigenesis. However, effects pyroptosis-related long noncoding RNAs (lncRNAs) on hepatocellular carcinoma (HCC) have not yet been completely elucidated. Based regression coefficients ZFPM2-AS1, KDM4A-AS1, LUCAT1, NRAV, CRYZL2P-SEC16B, AL031985.3, SNHG4, AL049840.5, AC008549.1, MKLN1-AS,...

Abstract Lymph node metastasis ( LNM) is a common invasive feature of hepatocellular carcinoma (HCC) associated with poor clinical outcomes. Through microarray profiling and bioinformatic analyses, we identified the circ-0044539-miR-29a-3p-VEGFA axis as potential key factor in progression HCC LNM. In cells nude mice, circ-0044539 downregulation or miR-29a-3p upregulation was small tumor size, PI3K-AKT-mTOR pathway inactivation, LNM factors (HIF-1α CXCR4). Furthermore, also responsible for...

Most genes are alternatively spliced and increasing number of evidences show that alternative splicing (AS) is modified related to tumor progression. Systematic profiles AS signature in hepatocellular carcinoma (HCC) absent urgently needed. Here, differentially transcripts between HCC non-HCC tissues were compared, prognosis-associated events by using univariate Cox regression analysis selected. Our gene functional enrichment demonstrated the potential pathways enriched survival-associated...

Our previous study demonstrated that heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a key gene facilitates metastasis of hepatocellular carcinoma (HCC). However, the molecular mechanisms behind this relationship are not fully understood. In our study, we utilized long-noncoding RNA (lncRNA) microarrays to identify HNRNPAB-regulated lncRNA named lnc-ELF209. findings from chromatin immunoprecipitation assays indicate HNRNPAB represses lnc-ELF209 transcription by directly binding its...

Inflammatory bowel disease (IBD) greatly affects human quality of life. Mannose has been reported to be used treat IBD, but the mechanism is currently unknown. C57/BL mice were as research subjects, and mouse acute colitis model was induced using dextran sulfate sodium salt (DSS). After oral administration mannose, body weights activity index (DAI) scores observed. The colon lengths, histopathological sections, fecal content microbial sequencing, epithelial inflammatory genes, tight junction...

The emergence of sorafenib resistance has become a predominant impediment and formidable dilemma in the therapeutic approach for hepatocellular carcinoma (HCC). Although approval next-generation drugs as alternatives to is significant development, concurrent use inhibitors that target additional key molecular pathways remains an effective strategy mitigate acquisition resistance. Here, we identified Glutathione S-Transferase Alpha 1 (GSTA1) critical modulator (SR) (HCC) based on our findings...

Although it has been known that the tumor microenvironment affects angiogenesis, precise mechanism remains unclear. In this study, we simulated of human esophageal squamous cell carcinoma (ESCC) by conditioned medium (TCM) to assess influence on normal endothelial cells (NECs). We found TCM-induced NECs showed enhanced angiogenic properties, such as migration, invasion and tube formation. Moreover, expressed (TECs) markers at higher levels, which indicated TCM probably promoted angiogenesis...

Abstract Colon cancer is one of the most malignant cancers worldwide. Nearly 20% all colon patients are diagnosed at stage IV (metastasis). However, further study difficult due to a lack understanding its pathogenesis. In this study, we acquired high–throughput sequence data from TCGA datasets and performed integrated bioinformatic analysis including differential gene expression analysis, ontology KEGG pathways protein–protein survival multivariate Cox proportional hazards regression in...

Early recurrence after hepatectomy for intrahepatic cholangiocarcinoma (ICC) has a great effect on the long-term survival outcomes of patients. We conducted this study to identify risk factors early and clarify whether is related ICC patients' outcomes. 322 patients who underwent partial at Liver Cancer Institute Zhongshan Hospital between January 2005 December 2011 were included in retrospectively study. was defined as within two years hepatectomy. identified total 168 with 23 late From...

Due to the phenotypic and molecular diversity of hepatocellular carcinomas (HCC), it is still a challenge determine patients' prognosis. We aim identify new prognostic markers for resected HCC patients. 274 patients were retrospectively identified samples collected from Zhongshan hospital, Fudan University. analyzed gene expression patterns tumors compared with patient survival times. "9-gene signature" associated by using coefficient regression formula multivariate Cox model. This signature...

Colorectal cancer (CRC) is the most common malignancy in gastrointestinal tract. The liver location of CRC metastases, which are main causes CRC-related death. However, mechanisms underlying metastasis to have not been characterized, resulting therapeutic challenges.The effects hepatic stellate cells (HSCs) on T were evaluated using vitro mixed lymphocyte reactions (MLRs) and cytokine production assays. HSC-induced CT26 cell migration proliferation vivo.HSCs induced hypo-responsiveness,...

In paraquat (PQ)‑induced acute lung injury (ALI)/<br />acute respiratory distress syndrome, PQ disrupts endothelial cell function and vascular integrity, which leads to increased pulmonary leakage. Anthrahydroquinone‑2,6‑disulfonate (AH2QDS) is a reducing agent that attenuates the extent of renal improves survival in PQ‑intoxicated Sprague‑Dawley (SD) rats. The present study aimed explore beneficial role AH2QDS PQ‑induced ALI its related mechanisms. A model was established using gavage SD...

Some patients with low rectal cancer experience anorectal and urogenital dysfunctions after surgery, which can influence the long-term quality of life. In this study, we aimed to protect nerve function in such scenarios by performing intraoperative monitoring pelvic autonomic nerves (IMPAN).We retrospectively investigated a series 87 undergoing laparoscopic anterior resection cancer. Nerve-sparing was evaluated both visually electrophysiologically. IMPAN performed stimulating under processed...

The study aimed to investigate the role of Long non-coding RNA (LncRNA) H19 in pathogenesis Diarrhea Irritable Bowel Syndrome (IBS-D), and further regulatory effect LncRNA on AQP1, 3 intestinal mucosa IBS-D patients, so as seek a new way elucidate mechanism IBS clinic.The levels H19, AQP3 were detected colonic tissues compared with that healthy controls. Through gene interference activation methods, small activating (saRNA) interfering (siRNA) transfered into Caco-2 cells vitro experiment,...

Background and aim: To investigate the value of aspartate aminotransferase-to-platelet ratio index (APRI) build a new nomogram for hepatocellular carcinoma (HCC) patients undergoing postoperative adjuvant transarterial chemoembolization (PATACE). Methods: We retrospectively reviewed 351 with HCC PATACE. collected baseline patient characteristics to obtain risk factors determining poor disease-free survival (DFS) early time recurrence (TTR) after The multivariate Cox proportional hazards...

Locally recurrent rectal cancer (LRRC) leads to a poor prognosis and appears as clinically predominant pattern of failure. In this research, whole-exome sequencing (WES) was performed on 21 samples from 8 patients search for the molecular mechanisms LRRC. The data analyzed by bioinformatics. Gene Expression Profiling Interactive Analysis (GEPIA) Human Protein Atlas (HPA) were validate candidate genes. Immunohistochemistry used detect protein expression LEF1 CyclinD1 in LRRC, primary (PRC),...

Emphysematous cystitis (EC) refers to a rare form of complicated urinary tract infection (UTI), which is typically observed in elderly females with severe diabetes mellitus. EC was triggered by bacteria, producing gas filled cysts the bladder wall and lumen, most common are Escherichia coli Klebsiella pneumoniae . Since could be potentially life-threatening, (like septic shock) can cause gangrenous (GC). This case report describes an 85-year-old female patient type 2 mellitus (DM) who...

Abnormal angiogenesis is one of the important hallmarks colorectal cancer as well other solid tumors. Optimally, anti-angiogenesis therapy could restrain malignant to control tumor expansion. PELP1 a scaffolding oncogenic protein in variety types, but its involvement unknown. In this study, was found be abnormally upregulated and highly coincidental with increased MVD CRC. Further, treatment conditioned medium (CM) from knockdown CRC cells remarkably arrested function human umbilical vein...