A5100733445- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Advanced Breast Cancer Therapies
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- BRCA gene mutations in cancer
- Cancer Treatment and Pharmacology
- DNA Repair Mechanisms
- Lung Cancer Treatments and Mutations
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- Genetic and phenotypic traits in livestock
- Estrogen and related hormone effects
- Reproductive Physiology in Livestock
- PARP inhibition in cancer therapy
Peking University
2016-2025
Peking University Cancer Hospital
2016-2025
Hainan Medical University
2017-2025
Chuzhou University
2024
Hebei Agricultural University
2024
Harbin Engineering University
2024
King University
2023-2024
Zhengzhou University
2024
Capital Medical University
2024
Xinjiang Medical University
2023
Abstract Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated expression profiles endometrial tissue on Days 9, 12 15 pregnancy Day non-pregnancy Yorkshire performed comprehensive analysis long non-coding RNAs (lncRNAs) samples using RNA sequencing. As result, 2805 novel lncRNAs, 2,376 (301 lncRNA 2075 mRNA) differentially expressed genes (DEGs) 2149 transcripts were obtained...
Hedgehog (Hh) signaling is crucially involved in tumorigenesis. This study aimed to assess the role of Hh regulation epithelial-mesenchymal transition (EMT), stemness properties and chemoresistance human pancreatic Panc-1 cancer stem cells (CSCs). were transfected with recombinant lentiviral vectors silence SMO serum-free floating-culture system was used isolate tumorspheres. The expression CSC EMT markers detected by flow cytometry, real-time RT-PCR Western blot analysis. Malignant...
The evolution of Large Language Models (LLMs) like ChatGPT and GPT-4 has sparked discussions on the advent Artificial General Intelligence (AGI). However, replicating such advancements in open-source models been challenging. This paper introduces InternLM2, an LLM that outperforms its predecessors comprehensive evaluations across 6 dimensions 30 benchmarks, long-context modeling, open-ended subjective through innovative pre-training optimization techniques. process InternLM2 is meticulously...
AimsEndothelial progenitor cells (EPCs) play a pivotal role in endothelial repair after artery injury. The chemokine receptor CXCR4 is key modulator of the homing EPCs to impaired and reendothelialization. In this study, we addressed hypothesis that gene transfer could enhance reendothelialization capacity EPCs.
The detection of circulating tumor cells (CTCs) with a specific antigen expression is necessary in therapeutic response monitoring and targeted therapy guidance. existence human epidermal growth factor receptor 2 (HER2) the concentration HER2-positive CTCs are strong indicators for patient diagnosis, prognosis, monitoring. Herein we report direct isolation by peptide-functionalized nanomaterials. We designed screened out peptide as an HER2 antibody alternative demonstrating high affinity...
Alterations in transcriptional regulators of glycolytic metabolism have been implicated brain tumor growth, but the underlying molecular mechanisms remain poorly understood.Knockdown and overexpression cells were used to explore functional roles HOXA3 cell proliferation, formation, aerobic glycolysis. Chromatin immunoprecipitation, luciferase assays, western blotting performed verify regulation HK2 PKM2 by HOXA3. PLA, Immunoprecipitation, GST-pull-down assays examine interaction KDM6A.We...
Background: MicroRNAs contribute to chemotherapy response in different types of cancer. We hypothesized that plasma miRNAs are potentially associated with patients metastatic breast Patients and Methods: Fourteen candidate microRNAs were chosen from the literature, their levels measured by quantitative polymerase chain reaction (PCR). Forty cancer as training groups. The potential significant validated another 103 samples. Results: In set, we identified 3 (miR-200a, miR-210, miR-451)...
Rationale: Considerable evidence suggests that breast cancer metastasis and recurrence occur due to emergence of stem cells (CSCs). In our previous study, we designed a high-throughput siRNA screening platform identifies inflammation genes involved in the regulation cell stemness. We reported CCL16 protein decreases OCT4 expression reduces ALDH+ subpopulation. However, mechanism by which maintains cell-like properties remains unclear. Methods: Tissue microarrays were used evaluate...
Recent clinical trials have suggested that solid cancers with mismatch repair (MMR) deficiency are highly responsive to immunotherapy, regardless of cancer types. Previous MMR-related studies on breast predominantly focused germline variants. However, the somatic MMR alterations not been comprehensively characterized in cancer. In this study, we integrated genomic, transcriptomic, and data from over 3000 cases across six public cohorts. Our findings revealed 1.2% harbored oncogenic...
Abstract Introduction: Hormone receptor (HR)-positive breast cancer is the most common subtype worldwide. While treatments with CDK4/6 inhibitors and endocrine therapies like aromatase (AIs) have improved patient outcomes, risk of disease progression persists. This study employs circulating tumor DNA (ctDNA) Next-Generation Sequencing (NGS) assays to profile gene variations quantify fraction in patients advanced HR-positive cancer, aiming identify relevant biomarkers enhance management....
Fibroblast-like synoviocytes (FLS) play a pivotal role in the pathogenesis of rheumatoid arthritis (RA) through aggressive proliferation and invasion, certain proinflammatory cytokines may affect synoviocyte proliferation. To evaluate whether interleukin-21 (IL-21) could promote cytokine production by RA-FLS, immunohistochemistry immunoblotting were performed to observe expression IL-21 receptor (IL-21R) synovial tissues FLS from RA osteoarthritis (OA) patients. The MTS assay was used...
Abstract Blood‐based detection and molecular phenotyping are highly desired for the early diagnosis dynamic monitoring of cancer. Extracellular vesicles (EVs) carry information from cells origin biomarkers However, analysis EVs has been challenging due to their nanoscaled size. Here, an assessment serum based on microbead‐assisted flow cytometry is established. The clinical utility this method validated in human epidermal growth factor receptor 2 (HER2) breast Good correlation between status...
The correlations between circulating tumour DNA (ctDNA)-derived genomic markers and treatment response survival outcome in Chinese patients with advanced breast cancer (ABC) have not been extensively characterized.Blood samples from 141 ABC who underwent first-line standard Peking University Cancer Hospital were collected. A next-generation sequencing based liquid biopsy assay (PredicineCARE) was used to detect somatic mutations copy number variations (CNVs) ctDNA. subset of matched blood...
Abstract Background Pyrotinib is currently approved for the treatment of HER2-positive advanced breast cancer in China. Data on overall survival (OS) and efficacy patients with brain metastasis (BM) remain scarce. This study evaluated effectiveness pyrotinib a real-world setting, especially BM. Methods We reviewed metastatic treated pyrotinib-based therapy between June 2018 2022. Progression-free (PFS), OS, objective response rate, safety were analyzed following administration pyrotinib....