A5004905094- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Neuroendocrine Tumor Research Advances
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Lung Cancer Research Studies
- Enhanced Recovery After Surgery
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gastrointestinal Tumor Research and Treatment
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Cell death mechanisms and regulation
- Gallbladder and Bile Duct Disorders
- Blood transfusion and management
- Gastrointestinal disorders and treatments
- Phagocytosis and Immune Regulation
- Intestinal and Peritoneal Adhesions
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Nanoparticle-Based Drug Delivery
- PARP inhibition in cancer therapy
Peking University
2014-2024
Peking University First Hospital
2014-2024
Sun Yat-sen University
2021
Third Affiliated Hospital of Sun Yat-sen University
2021
Capital Medical University
2007
BackgroundPancreatic ductal adenocarcinoma (PDAC) is most aggressive among all gastrointestinal tumors. The complex intra-tumor heterogeneity and special tumor microenvironment in PDAC bring great challenges for developing effective treatment strategies. We aimed to delineate dynamic changes of components during malignant progression utilizing single-cell RNA sequencing.MethodsA total 11 samples (4 I, 4 II, 3 III) were used construct expression matrix. After identifying distinct cell...
Purpose: Most patients with pancreatic ductal adenocarcinoma (PDAC) have vascular invasion and metastasis, leading to low surgical resection rate dismal prognosis. Tumor angiogenesis is related metastasis. However, anti-angiogenesis therapeutic effects in PDAC are limited. Therefore, it imperative explore molecular mechanism of PDAC. Experimental Design: scRNA-seq data were utilized delineatetranscriptional profiles endothelial cells The vitro vivo models used the role derived exosomes under...
Inter- and intra-tumor heterogeneity is a major hurdle in primary liver cancer (PLC) precision therapy. Here, we establish PLC biobank, consisting of 399 tumor organoids derived from 144 patients, which recapitulates histopathology genomic landscape parental tumors, reliable for drug sensitivity screening, as evidenced by both vivo models patient response. Integrative analysis dissects heterogeneity, regarding genomic/transcriptomic characteristics to seven clinically relevant drugs, well...
Pancreatic cancer (PCa) is one of the most lethal malignancies, with a 5 year survival rate less than 8%. Current treatment regiments have low response in unselected patients. However, subgroup PCa patients BRCA mutations may benefit from poly-ADP-ribose polymerase inhibitors (PARPi) due to their biological properties DNA repair. Dose-limiting toxicity normal tissues frequently observed when PARPi are combined other chemotherapies, and co-delivery two drugs tumor sites at an adequate...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers, characterized by rapid progression, metastasis, and difficulty in diagnosis. However, there are no effective liquid-based testing methods available for PDAC detection. Here we introduce a minimally invasive approach that uses machine learning (ML) lipidomics to detect PDAC. Through greedy algorithm mass spectrum feature selection, optimized 17 characteristic metabolites as detection features developed liquid...
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) has a complex tumor immune microenvironment (TIME), the clinical value of which remains elusive. This study aimed to delineate landscape PDAC and determine features in TIME. Methods Univariable multivariable Cox regression analyses were performed evaluate establish new prognostic model. We also conducted single-cell RNA sequencing (scRNA-seq) further characterize profiles explore cell-to-cell interactions. Results There was...
Nectin cell adhesion molecule 4 (nectin-4) is a transmembrane protein overexpressed on variety of cancers and plays an important role in oncogenic metastatic processes. The nectin-4–targeted antibody–drug conjugate enfortumab vedotin has been approved for treating locally advanced or urothelial cancer, but the efficacy other types cancer remains to be explored. aim this study was evaluate feasibility PET imaging with <sup>68</sup>Ga-N188 as noninvasive method quantify membranous nectin-4...
Abstract One major obstacle in the drug treatment of pancreatic ductal adenocarcinoma (PDAC) is its highly fibrotic tumor microenvironment, which replete with activated stellate cells (a‐PSCs). These a‐PSCs generate abundant extracellular matrix and secrete various cytokines to form biophysical biochemical barriers, impeding access tissues. Therefore, it imperative develop a strategy for reversing PSC activation thereby removing barriers facilitate PDAC treatment. Herein, by integrating...
The targeted delivery of hydrophobic therapeutic drugs to tumors is one the major challenges in drug development. use natural proteins as vehicles holds great promise due various functionalities proteins. In current study, we exploited a protein, GroEL, which possesses double layer cage structure, container, switchable by ATP binding hydrophilic status, design novel and intelligent molecular machine with controlled release profile. When loaded antitumor drug, Doxorubicin (Dox), GroEL was...
Objective: This multicenter randomized controlled trial was designed to test the hypothesis that early drain removal (EDR) could decrease incidence of grade 2 4 complications for patients undoing pancreaticoduodenectomy (PD) with low or intermediate risk postoperative pancreatic fistula (POPF). Background: The safety and effects EDR on after PD are still controversial. Methods: A at 6 tertiary referral hospitals carried out (NCT03055676). Patients who met inclusion criteria, including...
To assess the predictive capability of CT radiomics features for early recurrence (ER) pancreatic ductal adenocarcinoma (PDAC). Postoperative PDAC patients were retrospectively selected, all whom had undergone preoperative imaging and surgery. Both with resectable or borderline-resectable cancer met eligibility criteria in this study. However, owing to differences treatment strategies such, research mainly focused on cancer. All subject follow-up assessments a minimum 9 months. A total 250...
The incidence of small (≤2 cm), non-functioning pancreatic neuroendocrine tumors (NF-pNETs) increased in the last decades. Before making appropriate strategy for patients with NF-pNETs ≤2 cm, pathological confirmation is vital. Incidentally diagnosed, sporadic may bring aggressive behavior and poor prognosis, such as extrapancreatic extension, lymph nodal metastasis, distant metastasis recurrence, even causing disease-related death. Understanding formulating an small, have been controversial...
There are no reliable biomarkers for early diagnosis or prognosis evaluation in pancreatic ductal adenocarcinoma (PDAC). Multiple scRNA-seq datasets PDAC were retrieved from online databases and combined with results our previous study. The malignant cells identified through calculating copy number variation (CNV) scores. robust markers of found. Five immune-related signatures, including SPP1, LINC00683, SNHG10, LINC00237, CASC19, used to develop a risk score formula predict the overall...
Single-cell RNA sequencing (scRNA-seq) analysis have provided an unprecedented resolution for the studies on diabetic retinopathy (DR). However, early changes in retina diabetes remain unclear. A total of 8 human and mouse scRNA-seq datasets, containing 276,402 cells were analyzed individually to comprehensively delineate retinal cell atlas. The neural retinas isolated from type 2 (T2D) control mice, was conducted evaluate effects retina. Bipolar (BC) heterogeneity identified. We found some...
Single-cell RNA sequencing has paved the way for delineating pancreatic islet cell atlas and identifying hallmarks of diabetes. However, pathological alterations type 2 diabetes (T2D) remain unclear. We isolated islets from control T2D mice single-cell (scRNA-seq) retrieved multiple datasets open databases. The complete landscape robust marker genes transcription factors each endocrine were identified. GLRA1 was restricted to beta cells, cells exhibited obvious heterogeneity. subcluster in...
Mesopancreas is a controversial structure.This study aimed to explore the anatomical characteristics of mesopancreas, define range total mesopancreas excision (TMpE), and evaluate feasibility, safety effectivity TMpE in treatment pancreatic head cancer.The clinical pathological data 58 consecutive patients undergoing for carcinoma from January 2013 December 2015 were analyzed prospectively.The perioperative morbidity, mortality outcomes compared with conventional pancreaticoduodenectomy.The...
The 5-year overall survival (OS) of pancreatic ductal adenocarcinoma (PDAC) is only 10%, partly owing to the lack reliable diagnostic and prognostic biomarkers. raw gene-cell matrix for single-cell RNA-seq (scRNA-seq) analysis was downloaded from GSA database. We drew cell atlas PDAC normal tissues. inferCNV used distinguish tumor cells cells. identified differential expression genes (DEGs) by comparing common DEGs were conduct model using univariate multivariate Cox or logistic regression...
Clinically relevant postoperative pancreatic fistula (CR-POPF) is a severe complication which may be caused by perioperative nutrition problem. We aimed to study whether patients with high nutritional risk (NRS2002 score ≥ 5) might benefit from preoperative support regarding the of CR-POPF after open pancreaticoduodenectomy.Consecutive undergoing pancreaticoduodenectomy complete record NRS2002 at two Chinese institutions between 2013 and 2018 were analysed. was diagnosed following 2016 ISGPS...