A5100620188- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Calcium signaling and nucleotide metabolism
- Connexins and lens biology
- Virus-based gene therapy research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Innovative Educational Techniques
- Plant-derived Lignans Synthesis and Bioactivity
- Bacillus and Francisella bacterial research
- Higher Education and Teaching Methods
- Ferroptosis and cancer prognosis
- Educational Technology and Assessment
- Herpesvirus Infections and Treatments
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Sphingolipid Metabolism and Signaling
- Ginseng Biological Effects and Applications
- Cancer-related Molecular Pathways
- Immune responses and vaccinations
- Innovative Teaching Methods
- 14-3-3 protein interactions
- Technology and Security Systems
- Educational Technology and Pedagogy
Guangzhou University of Chinese Medicine
2013-2025
Markey Cancer Center
2016
Jinggangshan University
2009
Sun Yat-sen University
2009
Sun Yat-sen University Cancer Center
2009
Ningde Normal University
2008
Abstract Autophagy inhibition has been demonstrated to increase the efficacy of conventional chemotherapy. In this study, we identified hederagenin, a triterpenoid derived from Hedera helix , as potent inhibitor autophagy and then hypothesized that hederagenin might synergize with chemotherapeutic drugs (e.g., cisplatin paclitaxel) kill lung cancer cells. Firstly, observed induced increased autophagosomes in cells concomitantly upregulation LC3-II p62, which indicated impairment autophagic...
Cancer cells are characterized by malignant proliferation and aberrant metabolism thereby liable to the depletion of nutrients accumulation metabolic waste. To maintain cellular homeostasis, cancer prone upregulating canonical autophagy pathway. Here, we identified paroxetine hydrochloride (Paxil) as a late inhibitor investigated its killing effect on lung with xenograft mouse model in vivo. Upregulated LC3-II p62 expression indicated that Paxil inhibited autophagy. Acid-sensitive dyes...
Abstract Colorectal cancer (CRC) is one of the most common cancers worldwide. The tumor microenvironment exerts crucial effects in driving CRC progression. Cancer‐associated fibroblasts (CAFs) serve as important components promoting This study aimed to elucidate novel molecular mechanisms CAF‐secreted insulin‐like growth factor (IGF) 2 colorectal carcinogenesis. Our results indicated that IGF2 was a prominent upregulated CAFs compared with normal fibroblasts. CAF‐derived conditioned media...
Abstract MHC-I-mediated antigen presentation is pivotal in antitumor immunity, enabling the recognition and destruction of tumor cells by CD8 + T cells. Both proteasome autophagy serve as essential cellular degradation mechanisms that regulate stability functionality MHC-I molecules. In melanoma, modulating pathways affect can profoundly influence therapeutic outcomes immunotherapy. Our initial effort this study was a screening process to identify natural compounds capable amplifying surface...
Chemoresistance is a major limiting factor that impairs the outcome of non-small cell lung cancer (NSCLC) chemotherapy. Paclitaxel (Tax) induces protective autophagy in NSCLC cells, leading to development drug resistance. We recently identified new inhibitor (alpha-hederin) and hypothesized it may promote killing effect Tax on cells. found alpha-hederin (α-Hed) could block late autophagic flux cells by altering lysosomal pH inhibiting cathepsin D maturation. Combination treatment α-Hed...
Dioscin is a natural steroidal saponin that can be isolated from Chinese medicine, such as Dioscoreae rhizoma. It has wild range of pharmacological activities hepatoprotection, lipid-lowering effect, and anti-inflammation. Recently, mounting studies reported the anticancer effect dioscin on variety tumor cells. However, potential epithelial-mesenchymal transition (EMT) HepG2 cells unclear. In present study, was identified to inhibit transforming growth factor-β1 (TGF-β1) induced invasive...
The protein EPHB4 plays a vital role in various tumor types. However, few studies into the function of circ‐EPHB4 (hsa_circ_0001730) tumors have been conducted. This study aimed to investigate functions and underlying mechanism regulating hepatocellular carcinoma (HCC). expression was found be downregulated HCC tissues, whereas overexpression suppressed cell viability, induced apoptosis, inhibited migration invasion Huh7 HepG2 cells. levels were negatively correlated with weight, size,...
Abstract Cancer cells have developed chemoresistance and improved their survival through the upregulation of autophagic mechanisms that protect mitochondrial function. Here, we report traditional Chinese anticancer agent tubeimoside I (Tub), which is a potent inhibitor autophagy, can promote mitochondria-associated apoptosis in lung cancer cells. We found Tub disrupted both lysosomal pathways. One its was induction DRP1-mediated fragmentation. Another mechanism blocking late-stage flux via...
The cyclin-dependent kinase inhibitor p21(WAF1/CIP1) is a critical regulator of cell cycle, and it easily degraded by proteasome through ubiquitin-dependent -independent pathway. mechanism the post-translational regulation p21 stability remains to be further clarified. In present study, we have identified nucleophosmin (NPM)/B23, multifunctional protein that bound contributed its stability. direct interaction between NPM was confirmed reciprocal co-immunoprecipitation GST pull-down assay....
Suicide gene therapy is a promising strategy against melanoma. However, the low efficiency of transfer technique can limit its application. Our preliminary data showed that dioscin, glucoside saponin, could upregulate expression connexins Cx26 and Cx43, major components gap junctions, in melanoma cells. We hypothesized dioscin may increase bystander effect herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) through increasing formation junctions. Further analysis indeed junctional...
The main mechanistic function of most chemotherapeutic drugs is mediated by inducing mitochondria-dependent apoptosis. Tumor cells usually respond to upregulate autophagy eliminate impaired mitochondria for survival. Hypothetically, inhibiting might promote apoptosis, thus enhancing the efficacy therapies. We previously identified N-methylparoxetine (NMP) as an inducer mitochondrial fragmentation with subsequent apoptosis in non-small cell lung cancer (NSCLC) cells. discovered that ROS was...
Abstract Melanoma is the most lethal type of skin cancer. Despite breakthroughs in clinical treatment melanoma using tumor immunotherapy, many patients do not benefit from these immunotherapies because multiple immunosuppressive mechanisms. Therefore, there an urgent need to determine mechanisms tumor-immune system interactions and their molecular determinants improve cancer immunotherapy. In this study, combined analysis microarray data single-cell RNA sequencing revealed key between immune...
Despite its low transfer efficiency, suicide gene therapy with HSV-TK is known for bystander killing effect. The connexin-based gap junction believed to mediate the Recently, we found that resveratrol, a polyphenol compound, increased expression of Cx26 and Cx43, which are connexins important constituents junctions, in murine hepatoma cells. Hypothetically, resveratrol-induced upregulation junctions may improve effect HSV-TK/GCV has on Our present investigation revealed resveratrol could...
Abstract Systemic or local inflammation drives the pathogenesis of various human diseases. Small compounds with anti-inflammatory properties hold great potential for clinical translation. Over recent decades, many have been screened their action against inflammation-related targets. Databases that integrate physicochemical and bioassay results these are lacking. We created an “Anti-Inflammatory Compounds Database” (AICD) to deposit anti-inflammation activities. A total 232 targets were...
The bystander effect is an intriguing phenomenon by which adjacent cells become sensitized to drug treatment during gene therapy with herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV). This reported be mediated gap junctional intercellular communication (GJIC), and therefore, we postulated that upregulation of genes facilitate GJIC may enhance the HSV-tk/GCV effect. Previous findings have shown Tanshinone IIA (Tan IIA), a chemical substance derived from Chinese medicine herb,...
Objectives: This study aimed to investigate the precise role of miR-34b in nasopharyngeal carcinoma (NPC). Materials and methods: The expression transcription ubiquitin-specific peptidase 22 (USP22) were examined using quantitative reverse transcription-polymerase chain reaction. Western blot analysis was used measure protein USP22. A dual-luciferase assay interaction between Cell viability determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. cell cycle...
Background: The risk of co-epidemic between COVID-19 and influenza is very high, so it urgent to find a treatment strategy for the co-infection. Previous studies have shown that phillyrin can not only inhibit replication two viruses, but also has good anti-inflammatory effect, which expected become candidate compound against influenza. Objective: To explore possibility as co-infection speculate its potential regulatory mechanism. Methods: We used series bioinformatics network pharmacology...
Clinically, although chemotherapy is one of the most commonly used methods treating tumors, chemotherapeutic drugs can induce autophagic flux and increase tumor cell resistance, leading to drug tolerance. Therefore, theoretically, inhibiting autophagy may improve efficacy chemotherapy. The discovery regulators their potential application as adjuvant anti-cancer substantial importance. In this study, we clarified that Fangjihuangqi Decoction (FJHQ, traditional Chinese medicine) an inhibitor,...
Hepatocellular carcinoma (HCC) is the fifth most common cancer in world. Up to now, many genes associated with HCC have not yet been identified. In this study, we screened HCC-related through integrated analysis of TCGA database, which potential biomarkers were also further validated by clinical specimens. The discovery for provides more opportunities diagnostic indicators or gene-targeted therapies.Cancer-related Cancer Genome Atlas (TCGA) database a random forest (RF) classifier based on...