A5036116908- Ear Surgery and Otitis Media
- Respiratory and Cough-Related Research
- Tracheal and airway disorders
- Head and Neck Cancer Studies
- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Pneumonia and Respiratory Infections
- Immune Cell Function and Interaction
- Streptococcal Infections and Treatments
- Sinusitis and nasal conditions
- Polyomavirus and related diseases
- Otolaryngology and Infectious Diseases
- Nasal Surgery and Airway Studies
- Peptidase Inhibition and Analysis
- Clostridium difficile and Clostridium perfringens research
- Blood groups and transfusion
- Head and Neck Surgical Oncology
- T-cell and B-cell Immunology
- Congenital Ear and Nasal Anomalies
- Bacterial Infections and Vaccines
- Microscopic Colitis
- Viral-associated cancers and disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
Helsinki University Hospital
2015-2024
University of Helsinki
2015-2024
Kainuun keskussairaala
2005-2012
Medicel (Switzerland)
2009
Sapporo Medical University
2005
Stanford University
1990-1992
Dana-Farber Cancer Institute
1990
Institute for Molecular Medicine Finland
1988
Proliferation and immunologic function of T lymphocytes are initiated by signals from the antigen receptor that inhibited immunosuppressant FK506 but not its structural analog, rapamycin. On other hand, interleukin 2 (IL-2)-induced blocked rapamycin FK506. Remarkably, these two drugs inhibit each other's actions, raising possibility both act means a common immunophilin (immunosuppressant binding protein). We find dissociation constant to protein FKBP (Kd = 0.2 nM) is close 0.4 their...
Stimulation of T lymphocytes through their antigen receptor leads to the appearance several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate activity a single factor individual viable cells, we have applied an assay that uses fluorescence-activated cell sorter quantitate beta-galactosidase (beta-gal). We analyzed distribution transcriptional among cells undergoing activation by using construct...
Glucocorticoid-dependent transcriptional enhancement is known to occur through the interaction of glucocorticoid receptor (GR) with specific DNA response elements. In contrast, negative regulation gene expression by this class hormone less well understood. Glucocorticoids are potent immunosuppressive agents acting primarily inhibiting T lymphocyte activation and lymphokine production. Interleukin 2 (IL-2) expression, a critical early event during activation, inhibited in...
Background. Clostridium difficile causes diarrhea that ranges from a benign, self-limiting antibiotic use–associated disease to life-threatening pseudomembranous colitis. has rarely been isolated in extraintestinal infections. Our objective was characterize clinical features and risk factors of these
The solid-phase radioimmunoassay for human antibodies was improved so that it gave the total concentration as well concentrations of IgM, IgA, IgG1, IgG2, IgG3 and IgG4 antibodies, all seven in same "L units"/ml. This accomplished by standardization monoclonal with monoisotypic rather than myelomas; myelomas were found unsatisfactory this purpose. For first time possible to determine isotype composition an antibody population percent terms. weight equivalent L-unit one hyperimmune tetanus...
Abstract Exercise‐induced bronchoconstriction (EIB) is a specific sign of active asthma, but its assessment in young children may be difficult with lung function techniques requiring cooperation. The aim the study was to assess normal pattern exercise‐induced responses respiratory impedance by using impulse oscillometry (IOS), and investigate how these discriminate wheezy from control subjects. IOS measurements were performed consecutive sample aged 3–7 years (n = 130) an matched group...
Trichodysplasia spinulosa (TS)-associated polyomavirus (TSV) was recently (in 2010) discovered in TS lesions. To investigate the seroprevalence and primary exposure time of this virus, we set up a virus protein (VP1) viruslike particle (VLP)-based immunoglobulin G enzyme immunoassay. The TSV 5%, among children aged 1-4 years, rising to 48% at 6-10 70% 149 adults. antibodies did not cross-react with corresponding Merkel cell VLPs, their reactivity appeared conformational. circulates widely...
Summary Background Clostridium difficile can cause severe antibiotic‐associated colitis. Conventional treatments with metronidazole and vancomycin improve symptoms, but after discontinuation of treatment, C. infection ( CDI ) recurs in a number patients. Rifaximin is rifamycin‐based non‐systemic antibiotic that has effect against . Aim To assess the effectiveness rifaximin recurrent infection. Methods We retrospectively evaluated records 32 patients who were treated for The symptoms 12 weeks...
Objectives: Oropharyngeal squamous cell carcinoma (OPSCC) is divided in two different disease entities depending on HPV involvement. We investigated differences presenting symptoms and clinical findings patients with HPV-positive -negative OPSCC tumors.Methods: Altogether 118 consecutive diagnosed primary between 2012 2014 at the Helsinki University Hospital were included. HPV-status of tumors was assessed by PCR detection DNA immunostaining p16-INK4a antibody.Results: Fifty-one (47.7%) had...
Objective. To evaluate the efficacy of adenoidectomy in reducing incidence otitis media among children who are younger than 4 years and receive tympanostomy tubes. Methods. A randomized trial was conducted at a tertiary center clinic. total 217 were aged 12 to 48 months had recurrent acute (>3 episodes during past 6 months) or chronic with effusion, no obstructive symptoms as result adenoid enlargement, previous surgical intervention enrolled study. Adenoidectomy conjunction insertion...
The role of protein kinase C (PKC) in TNFα‐induced activation endothelial adhesion molecules ICAM‐1 and VCAM‐1 was analysed. Phorbol myristate acetate, which is known to activate PKC, able lo mimic up‐regulation partly also expression. Similarly a PKC inhibitor, H7, but not another inhibitor. HA1004, inhibited enhancement expression at both the mRNA level. Moreover we were measure transient peak 16 min after TNFα induction cells analysed by phorbol‐dibutyrate binding. These results indicate...
Antibody responses of young adults to Haemophilus influenzae type b polysaccharide (Hib) or its protein conjugate were studied with special attention the isotype composition antibodies. Three conclusions interest can be made: 1) Immunoglobulin G (IgG) antibodies in polysaccharide-immunized volunteers displayed subclass pattern previously found meningococcal A polysaccharide. IgG1 was predominant IgG some individuals, IgG2 others. Still others had two subclasses varying but more even...
The association between exercise-induced bronchoconstriction (EIB) and exhaled nitric oxide (FE(NO)) has not been investigated in young children with atopic or non-atopic wheeze, two different phenotypes of asthma the early childhood. Steroid naïve 3- to 7-yr-old recent wheeze (n = 84) age-matched control subjects without respiratory symptoms 71) underwent exercise challenge test, measurement FE(NO) skin prick testing (SPT). EIB was assessed by using impulse oscillometry, standard online...
Background: Fusobacterium species are anaerobic bacteria that relatively rarely cause sepsis with a variable clinical presentation. Methods: We reviewed the records of 52 consecutive patients who had bacteraemia over 10-y period. Results: The pictures could be classified into 4 groups: (1) Lemierre's syndrome necrophorum and internal jugular vein thrombosis, n = 5 (10%); (2) previously healthy F. without any signs macroscopic vascular thrombosis (but them abscesses), 14 (27%); (3) women...
Background Predisposition to childhood otitis media (OM) has a strong genetic component, with polymorphisms in innate immunity genes suspected contribute risk. Studies on several have been conducted, but most associations failed replicate independent cohorts. Methods We investigated 53 gene Finnish cohort of 624 cases and 778 controls. A positive association signal was followed up tagging approach tested an 205 cases, British 1269 trios, as well two cohorts from the United States (US); one...
This is the first controlled study of balloon sinuplasty's long-term efficacy with follow-up time over 5 years. The results are in accordance a previous 2-year-follow-up study. Both techniques retained and patient satisfaction on average 6 years after surgery.Endoscopic sinus surgery (ESS) sinuplasty considered as treatment for chronic rhinosinusitis (CRS) failure conservative therapy. High cost lack studies restrain use sinuplasty.The aim this was to compare CRS patients who had undergone...