A5078571293- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Retinal and Optic Conditions
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Intraocular Surgery and Lenses
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Angiogenesis and VEGF in Cancer
- Vascular Malformations Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Apelin-related biomedical research
- MicroRNA in disease regulation
- Blood Coagulation and Thrombosis Mechanisms
- Extracellular vesicles in disease
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Lipid metabolism and disorders
- Circular RNAs in diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Atherosclerosis and Cardiovascular Diseases
- Ocular and Laser Science Research
- Immune responses and vaccinations
- Neuropeptides and Animal Physiology
- Retinopathy of Prematurity Studies
Osaka University
2014-2025
Scripps Research Institute
2015-2020
ShanghaiTech University
2017
Osaka National Hospital
2007-2009
Functional interactions between neurons, vasculature, and glia within neurovascular units are critical for maintenance of the retina other CNS tissues. For example, architecture neurosensory is a highly organized structure with alternating layers neurons blood vessels that match metabolic demand neuronal activity an appropriate supply oxygen perfused blood. Here, using murine genetic models cell ablation strategies, we have demonstrated subset retinal interneurons, amacrine horizontal cells,...
Outer retinal and renal glomerular functions rely on specialized vasculature maintained by VEGF that is produced neighboring epithelial cells, the pigment epithelium (RPE) podocytes, respectively. Dysregulation of RPE- podocyte-derived associated with neovascularization in wet age-related macular degeneration (ARMD), choriocapillaris degeneration, thrombotic microangiopathy (TMA). Since complement activation genetic variants inhibitory factor H (CFH) are also features both ARMD TMA, we...
Anti-angiogenic drugs targeting vascular endothelial cell growth factor receptor have provided modest clinical benefit, in part, owing to the actions of additional angiogenic factors that stimulate tumour neoangiogenesis parallel. To overcome this redundancy, approaches these other signalling pathways are required. Here we show, using cell-targeted mice, small GTPase Arf6 is required for hepatocyte (HGF)-induced and growth. deletion from cells abolishes HGF-stimulated β1 integrin recycling....
Introduction: Proliferative vitreoretinopathy (PVR) is a condition in which proliferation forms after rhegmatogenous retinal detachment (RRD) surgery or old RRD, and sometimes, PVR can occur under silicone oil (SO). Here, we report 2 cases of evaluated preoperative B-scan images SO using ultra-widefield swept-source OCT (UWF-SS-OCT). Case Presentation: The first case was 61-year-old male who presented with tamponade multiple pars plana vitrectomies (PPVs) at the previous hospital, second...
Interactions between astrocytes and endothelial cells (ECs) are crucial for retinal vascular formation. Astrocytes induce migration proliferation of ECs via their production growth factor (VEGF) and, conversely, maturation possibly by the secretion leukemia inhibitory (LIF). Together with astrocytes, this finalizes angiogenesis. Thus far, mechanisms triggering LIF in unclear. Here we show that apelin, a ligand receptor APJ, induces mediated from ECs. APJ (Aplnr)- Apln-deficient mice delayed...
The retina consists of organized layers photoreceptors, interneurons, glia, epithelial cells, and endothelial cells. economic model supply demand used to appropriately determine cost is highly applicable the retina, in which extreme metabolic demands phototransduction are met by precisely localized designed vascular networks. Proper development maintenance these networks critical normal visual function; dysregulation characteristic several devastating human diseases, including but not...
Vascular abnormalities are a common component of eye diseases that often lead to vision loss. Vaso-obliteration is associated with inherited retinal degenerations, since photoreceptor atrophy lowers local metabolic demands and vascular support those regions no longer required. Given the degree neurovascular crosstalk in retina, it may be possible use one cell type rescue another face severe stress, such as hypoxia or genetically encoded cell-specific degenerations. Here, we show...
Abnormal subretinal neovascularization is a characteristic of vision-threatening retinal diseases, including macular telangiectasia (MacTel) and angiomatous proliferation (RAP). Subretinal neovascular tufts photoreceptor dysfunction are observed in very-low-density lipoprotein receptor (Vldlr–/–) mutant mice. These changes mirror those patients with MacTel RAP, but the pathogenesis largely unknown. In this study, we show that microglia were closely associated Vldlr–/– mice tissue from...
Purpose To investigate the effect of an intravitreally administered CCR2 antagonist, INCB3344, on a mouse model choroidal neovascularization (CNV). Methods CNV was induced by laser photocoagulation Day 0 in wild type mice. INCB3344 or vehicle immediately after application. On 14, areas were measured retinal pigment epithelium (RPE)-choroid flat mounts and histopathologic examination performed 7 µm-thick sections. Macrophage infiltration evaluated immunohistochemistry RPE-choroid quantified...
To characterize wide-field optical coherence tomography angiography (OCTA) features of retinal nonperfusion in eyes with branch vein occlusion (BRVO).Automated scanning five 12 × 12-mm areas swept-source OCTA and fluorescein (FA) images was performed a consecutive case series 27 patients BRVO this institutional cross-sectional study. The correlation between the detected by both examinations assessed. Panoramic obtained scans were binarized or skeletonized, associations vascular parameters...
Microglia are the resident immune cells of central nervous system (CNS). They govern immunogenicity retina, which is considered to be part CNS; however, it not known how microglia develop in eye. Here, we studied human-induced pluripotent stem (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) partially mimics human eye development. Our results indicated microglia-like cells, have characteristics yolk-sac-like linage naturally 2D eye-like SEAM...
Ischemia-induced angiogenesis contributes to various neuronal and retinal diseases, often results in neurodegeneration visual impairment. Current treatments involve the use of anti-VEGF agents but are not successful all cases. In this study we determined that miR-30a-5p is another important mediator angiogenesis. Using a rodent model ischemic retinopathy, show inhibiting reduces neovascularization promotes tissue repair, through modulation microglial endothelial cell cross-talk. inhibition...
Purpose.: To assess the morphology of areas complete retinal nonperfusion in eyes with branch vein occlusion (BRVO) by en face images optical coherence tomography (OCT). Methods.: Forty-six BRVO that underwent swept-source OCT (SS-OCT) and fluorescein angiography were enrolled. Depth-integrated neural retina delineated automated segmentation algorithm obtained using SS-OCT. The findings a 6 × 6-mm area on SS-OCT scans at evaluated. Results.: Retinal was detected 25 eyes. Of these, 20 (80%)...
Anti-angiogenic biologicals represent an important concept for the treatment of vasoproliferative diseases. However, need continued treatment, presence nonresponders, and risk long-term side effects limit success existing therapeutic agents. Although Tspan12 has been shown to regulate retinal vascular development, nothing is known about its involvement in neovascular disease potential as a novel target diseases.Rodent models disease, including mouse model oxygen-induced retinopathy very low...
To compare the anatomic and functional outcomes of pars plana vitrectomy (PPV) for treating rhegmatogenous retinal detachments (RRDs) between two groups with without postoperative prone positioning.This retrospective cohort study included 142 eyes patients a primary RRD. All underwent PPV 20% sulfur hexafluoride gas tamponade were divided into groups: that did not maintain position postoperatively. followed more than 3 months. The main outcome measures best-corrected visual acuity (BCVA),...
We evaluated regression of iris neovascularization (INV) using en-face anterior-segment optical coherence tomography angiography (AS-OCTA) after anti-vascular endothelial growth factor (VEGF) therapy. Seven consecutive eyes with INV were examined before and anti-VEGF therapy, all AS-OCTA scans obtained a swept-source OCTA system an lens adapter. Slit-lamp microscopy photography anterior indocyanine green also performed. Quantitative analyses the vascular density, lacunarity, fractal...
Abstract We report to evaluate if disorganization of the retinal inner layers (DRIL) obtained by swept-source optical coherence tomography (SS-OCT) predicts postoperative best-corrected visual acuity (BCVA) treat proliferative diabetic retinopathy (PDR). Twenty-one eyes 21 patients who underwent vitrectomy for PDR were studied retrospectively. BCVA and SS-OCT images until 6 months postoperatively. The associations between parameters measured in a 1-mm central foveal area evaluated. DRIL...
Disruption of the neurovascular unit (NVU) underlies pathophysiology various CNS diseases. One strategy to repair NVU dysfunction uses stem/progenitor cells provide trophic support NVU's functionally coupled and interdependent vasculature surrounding parenchyma. A subset endothelial progenitor cells, colony-forming (ECFCs) with high expression CD44 hyaluronan receptor (CD44hi), provides such neurovasculotrophic via a paracrine mechanism. Here, we report that bioactive extracellular vesicles...
To investigate the role of apelin-APJ system in development choroidal neovascularization (CNV).Experimental CNV was induced by laser photocoagulation wild-type (WT), apelin-deficient (apelin-KO), and apelin receptor (APJ)-deficient (APJ-KO) mice. The gene expression levels angiogenic or inflammatory factors were determined quantitative real-time reverse transcription-polymerase chain reaction. APJ lesions examined immunohistochemistry. sizes three mouse models measured compared...