A5052752944- Advanced Glycation End Products research
- Cardiac Valve Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac pacing and defibrillation studies
- Atrial Fibrillation Management and Outcomes
- Diabetic Foot Ulcer Assessment and Management
- Aortic aneurysm repair treatments
- Migration, Health and Trauma
- Electrospun Nanofibers in Biomedical Applications
- Blood Coagulation and Thrombosis Mechanisms
- Interprofessional Education and Collaboration
- Cardiac and Coronary Surgery Techniques
- S100 Proteins and Annexins
- Proteoglycans and glycosaminoglycans research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Surgical Sutures and Adhesives
- Chemokine receptors and signaling
- Long-Term Effects of COVID-19
- Retinoids in leukemia and cellular processes
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Fibrosis and Remodeling
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2023
Associazione Italiana Arbitri
2021
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2020
Vita-Salute San Raffaele University
2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
San Raffaele University of Rome
2004-2009
Istituto Dermopatico dell'Immacolata
2000-2007
Centro Cardiologico Monzino
2002-2007
Fondazione Roma
2007
New York Medical College
2000-2005
High mobility group box 1 (HMGB1) is an abundant chromatin protein that acts as a cytokine when released in the extracellular milieu by necrotic and inflammatory cells. Here, we show HMGB1 its receptor for advanced glycation end products (RAGE) induce both migration proliferation of vessel-associated stem cells (mesoangioblasts), thus may play role muscle tissue regeneration. In vitro, induces adult embryonic mesoangioblasts, disrupts barrier function endothelial monolayers. living mice,...
High-mobility group box 1 protein (HMGB1) is a chromatin that released by inflammatory and necrotic cells. Extracellular HMGB1 signals tissue damage, stimulates the secretion of proinflammatory cytokines chemokines, modulates stem cell function. The present study examined exogenous effect on mouse left-ventricular function myocyte regeneration after infarction. Myocardial infarction was induced in C57BL/6 mice permanent coronary artery ligation. After 4 hours animals were reoperated 200 ng...
Tissue damage is usually followed by healing, as both differentiated and stem cells migrate to replace dead or damaged cells. Mesoangioblasts (vessel-associated that can repair muscles) fibroblasts toward soluble factors released tissue. Two such are high mobility group box 1 (HMGB1), a nuclear protein undergoing unscheduled death (necrosis) but not apoptotic cells, stromal derived factor (SDF)–1/CXCL12. We find HMGB1 activates the canonical κB (NF-κB) pathway via extracellular...
High mobility group box 1 protein (HMGB1) is a chromatin component leaked out by necrotic cells and actively secreted activated myeloid cells. The extracellular potent mediator of tissue remodeling. We show here that human atherosclerotic plaques, but not normal arteries, produce HMGB1. Secreted HMGB1 originates from endothelial cells, neointimal foam also smooth muscle (SMCs). SMCs are an unexpected source for HMGB1, since they normally express much lower amounts than other types, do...
Inhibitor of NF-kappaB kinases beta (IKKbeta) and alpha (IKKalpha) activate distinct signaling modules. The IKKbeta/canonical pathway rapidly responds to stress-like conditions, whereas the IKKalpha/noncanonical controls adaptive immunity. Moreover, IKKalpha can attenuate IKKbeta-initiated inflammatory responses. High mobility group box 1 (HMGB1), a chromatin protein, is an extracellular signal tissue damage-attracting cells in inflammation, regeneration, scar formation. We show that IKKbeta...
Administration of angiogenic factors stimulates neovascularization in ischemic tissues. However, there is no evidence that angiogenesis can be induced normoperfused skeletal muscles. We tested the hypothesis adenovirus-mediated intramuscular (IM) gene transfer 121-amino-acid form vascular endothelial growth factor (AdCMV.VEGF(121)) could stimulate nonischemic muscle and consequently attenuate hemodynamic deficit secondary to surgically ischemia.Rabbits rats received IM injections...
High mobility group box 1 protein (HMGB1) is a cytokine released by necrotic and inflammatory cells in response to injury. We examined the role of HMGB1 skeletal muscle regeneration after hindlimb ischemia.Unilateral ischemia was induced mice femoral artery dissection. levels increased regenerating blockade endogenous administration its truncated form, BoxA, resulted reduction vessel density. In contrast, intramuscular enhanced perfusion number fibers. To separately study myogenic angiogenic...
After endovascular injury, smooth muscle cells (SMCs) may be exposed to hemodynamic shear stress (SS), and these forces modulate neointima accumulation. The effect of SS on SMC migration invasion is unknown, it was examined in the present study.Bovine aortic SMCs were laminar 12 dyne/cm(2) for 3 (SS3) or 15 (SS15) hours; control (C3 C15) kept under static conditions. Platelet-derived growth factor (PDGF)-BB-directed evaluated by a modified Boyden chamber assay with filters coated either...
HMGB1 is a nuclear protein that signals tissue damage, as it released by cells dying traumatically or secreted activated innate immunity cells. Extracellular elicits the migration to site of damage several cell types, including inflammatory and stem The identity signaling pathways extracellular not known completely: We reported previously ERK NF-kappaB are involved, we report here Src also activated. ablation inhibition with kinase inhibitor PP2 blocks toward HMGB1. associates mediates...
High mobility group B box (HMGB) proteins are a family of chromatin made up two basic DNA binding domains, HMG A and B, C-terminal acidic tail. HMGB have highly conserved sequence, but different expression pattern: HMGB1 is almost ubiquitous, whereas the others expressed in only few tissues adults. We previously demonstrated that released by necrotic cells has chemoattractant activity for inflammatory stem cells, via to receptor advanced glycation endproducts (RAGE). can be actively secreted...
In the present study, we analyzed effect of conditioned media (CM) from bovine aortic endothelial cells exposed to laminar shear stress (SS) 5 dyne/cm2 (SS5) or 15 (SS15) for 16 hours on smooth muscle cell (SMC) migration. response CM SS5 (CMSS5) and SS15 (CMSS15), migration was 45 +/- 5.5 30 1.5 per field, respectively (P<0.05). Similar results were obtained with SS 2 versus 20 also when lasted 24 hours. Platelet-derived growth factor (PDGF)-AA levels in CMSS5 CMSS15 9 7 18 ng/10(6) hours,...
Abstract —The effect of retinoic acid (RA) on endothelial cells is still controversial and was examined in the present study. In bovine aortic (BAECs), all-trans RA (ATRA) 9- cis (9CRA), but not 13- (13CRA), induced fibroblast growth factor-2 (FGF-2) production exhibited a biphasic dose-dependent to enhance BAEC proliferation differentiation into tubular structures reconstituted basement membrane proteins (Matrigel); both processes were inhibited by FGF-2–neutralizing antibody. The pan...
ABSTRACT In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast factor (bFGF) are released locally modulate vascular smooth muscle cells (SMC) proliferation migration within the wall. The aim of present in vitro study was determine how rat aorta SMC respond simultaneous exposure PDGF-BB bFGF. a modified Boyden chamber assay bFGF exhibited dose-dependent effect inhibit chemotactic action PDGF-BB. A comparable result observed assays. contrast,...
Background: The CTSN (Cardiothoracic Surgical Trials Network) recently reported no difference in left ventricular end-systolic volume index or survival at 2 years between patients with severe ischemic mitral regurgitation (MR) randomized to valve repair replacement. However, replacement provided more durable correction of MR and fewer cardiovascular readmissions. Yet, cost-effectiveness outcomes have not been addressed. Methods Results: We conducted a analysis the surgical treatment based on...
Toxoplasmosis is a parasitic disease affecting wide range of species, including humans, and can be responsible for important clinical manifestations such as abortion neurological signs. Sheep show remarkable susceptibility to its causative agent, Toxoplasma gondii, zoonotic transmission may occur in case consumption undercooked meat obtained from infected animals. gondii seroprevalence sheep significantly vary on geographical basis, shown by numerous surveys conducted worldwide. To...