A5101982160- Calcium Carbonate Crystallization and Inhibition
- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Bone Tissue Engineering Materials
- MicroRNA in disease regulation
- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Parasite Biology and Host Interactions
- Paleontology and Stratigraphy of Fossils
- Algal biology and biofuel production
- Aquatic Invertebrate Ecology and Behavior
- 14-3-3 protein interactions
- melanin and skin pigmentation
- Marine Ecology and Invasive Species
- Plant Virus Research Studies
- Ocean Acidification Effects and Responses
- FOXO transcription factor regulation
- Magnesium Alloys: Properties and Applications
- Calpain Protease Function and Regulation
- Circular RNAs in diseases
- Calcium signaling and nucleotide metabolism
- Corrosion Behavior and Inhibition
Guangdong Ocean University
2015-2025
Zhanjiang Experimental Station
2015-2024
Hainan Marine Monitoring and Forecasting Center
2023
Institute of Oceanology
2012-2017
Chinese Academy of Sciences
2012-2017
University of Chinese Academy of Sciences
2017
The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, Mollusca. Here we report sequencing and assembly genome using short reads a fosmid-pooling strategy, along with transcriptomes development stress response proteome shell. is highly polymorphic rich in repetitive sequences, some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set genes responding environmental stress. expansion...
Abstract Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process matrix-assisted biomineralization. Despite recent advances, many aspects biomineralization its evolutionary origin remain unknown. The pearl oyster Pinctada fucata martensii a well-known master biomineralization, but molecular mechanisms that underlie production are not fully understood. We sequenced highly polymorphic genome conducted multi-omic biochemical studies to...
Abstract Pearl oysters are found in the marine ecosystem. Due to their frequent exposure microbial pathogens and environmental perturbations, they have developed a multifaceted innate immune system with differentially expressed immune‐related genes response range of stress via vast coordinated reactions, such as recognition, signal transduction, synthesis antimicrobial peptides, encapsulation phagocytosis circulating haemocytes. The species farmed produce pearls. With increasing adoption...
Mollusk shell is one kind of potential biomaterial, but its vague mineralization mechanism hinders further application. matrix proteins are important functional components that embedded in the shell, which play roles formation. The proteome oyster had been determined based on genome sequence by our group and gives chance for deep study this area. classical model formation posits mantle-secreted. But, study, we analyzed data combination with organ transcriptome found may be produced multiple...
Biomineralization-controlled exo-/endoskeleton growth contributes to body and size diversity. Molluscan shells undergo ectopic biomineralization form the exoskeleton biocalcified "pearl" involved in invading defence. Notably, exo-/endoskeletons have a common ancestral origin, but their regulation are largely unknown. This study employed pearl oyster, Pinctada fucata marntensii, widely used experimental model for invertebrates, perform whole-genome resequencing of 878 individuals from wild...
miR-29a is a conserved miRNA that participates in bone formation and immune response vertebrates. of Pinctada martensii (Pm-miR-29a) was identified the previous research though deep sequencing. In this report, precise sequence mature Pm-miR-29a validated using rapid amplification cDNA ends (miR-RACE) technology. The precursor predicted to have 87 bp. Stem loop qRT-PCR analysis showed easily detected all tissues, although expressions mantle gill were low. microstructure disrupted growth nacre...
MicroRNAs (miRNAs) are a class of non-coding RNA molecules with presumed post-transcriptional regulatory activity in various biological processes, such as development and biomineralization. Pinctada martensii is one the main species cultured for marine pearl production China Japan. In our previous research, 258 pm-miRNAs had been identified by solexa deep sequencing P. martensii, while it far from number miRNAs found other species. this study, based on transcriptome database sac, we 30...
Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) 294 an open reading fragment 1290 bp encoding 429 amino acid polypeptide and 3'-UTR 1388 bp. The deduced sequence Pm-BMP7 contained signal peptide, pro-domain mature peptide. peptide consisted 135 acids included transforming growth factor β family domain...
MicroRNAs (miRNAs) are noncoding RNA molecules that function as negative regulators of target genes. In our previous research, 258 pm-miRNAs were identified in Pinctada martensii by Solexa deep sequencing. Pm-miR-2305 was one the with a potential biomineralization. present study, precursor pm-miR-2305 predicted 96 bp, containing characteristic hairpin structure. Stem-loop qRT-PCR analysis indicated constitutively expressed all tissues (adductor muscle, gill, mantle, hepatopancreas, foot, and...
Heterodimeric PEBP2/CBFs are key regulators in diverse biological processes, such as haematopoietic stem-cell generation, bone formation and cancers. In this work, we cloned runt-like transcriptional factor (designated PmRunt) CBF β PmCBF) gene, which comprise the heterodimeric Pinctada martensii. PmRunt was identified with an open reading frame that encodes 545 amino acids has typical Runt domain. Phylogenetic analysis results speculated factors (RDs) vertebrates invertebrates separated...
The paired-box 3 (Pax3) is a transcription factor and it plays an important part in melanin synthesis. In this study, new Pax3 gene was identified from Pteria penguin (Röding, 1798) (P. penguin) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) its effect on synthesis deliberated RNA interference (RNAi). PpPax3 2250 bp long, containing open reading fragment 1365 encoding 455 amino acids. Amino acid alignment phylogenetic tree showed shared the highest (69.2%) identity...
Tumor necrosis factor receptor-associated 6 (TRAF6) is a key signaling adaptor molecule for tumor receptor superfamily and Toll-like receptor/interleukin-1 family members.It signals the upstream receptors involved in wide range of biological functions, such as immunity bone metabolism.In this report, TRAF6 gene from pearl oyster Pinctada martensii (designated PmTRAF6) was identified characterized.The obtained full-length PmTRAF6 cDNA 2273 bp, containing 5'-untranslated region (UTR) 297...
Marine pearl production is directly influenced by the growth speed of Pinctada fucata martensii. However, slow rate this organism remains main challenge in aquaculture production. Epidermal factor receptor (EGFR), an important tyrosine kinases animals, plays versatile functions development, and tissue regeneration. In study, we described characteristic function EGFR gene identified from P. f. martensii (PmEGFR). PmEGFR possesses a typical structure expressed all studied tissues, with highest...
Amusium pleuronectes (Linnaeus) that secretes red- and white-colored valves in two branches of mantle tissues is an excellent model for shell color research. High-throughput transcriptome sequencing profiling were applied this project to reveal the detailed molecular mechanism phenotype differentiation. In study, 50,796,780 54,361,178 clean reads generated from left branch (secreting red valve, RS) right white WS) using Illumina Hiseq 2000 platform. De novo assembly 149,375 176,652 unigenes...