A5040021434- Calcium signaling and nucleotide metabolism
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Pancreatic function and diabetes
- PARP inhibition in cancer therapy
- Neonatal Health and Biochemistry
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Receptor Mechanisms and Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Nitric Oxide and Endothelin Effects
- Cannabis and Cannabinoid Research
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Renin-Angiotensin System Studies
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Vibrio bacteria research studies
- Neurobiology and Insect Physiology Research
- Eicosanoids and Hypertension Pharmacology
- Alcohol Consumption and Health Effects
Jeonbuk National University
2013-2023
Wonkwang University
2022-2023
Jeonju University
2017-2021
Creative Research
2014-2018
Institute of Cytochemistry and Molecular Pharmacology
2015
Chonbuk National University Hospital
2006
The Wistar Institute
1994
National Institutes of Health
1989-1991
National Heart Lung and Blood Institute
1989-1991
University of Utah
1987-1988
We have cloned human cDNA encoding double-stranded RNA adenosine deaminase (DRADA). DRADA is a ubiquitous nuclear enzyme that converts multiple adenosines to inosines in double-helical substrates without apparent sequence specificity. The A --> I conversion activity of the protein encoded by was confirmed recombinant expression insect cells. Use DNA as molecular probe documented conservation across mammals and detected single transcript 7 kb all tissues analyzed. deduced primary structure...
Doxorubicin (DOX) is one of the most potent anticancer drugs and induces acute cardiac arrhythmias chronic cumulative cardiomyopathy. Though DOX-induced cardiotoxicity known to be caused mainly by ROS generation, a disturbance Ca2+ homeostasis also implicated cardiotoxic mechanisms. In this study, molecular basis modulation intracellular concentration ([Ca2+]i) was investigated. Treatment adult rat cardiomyocytes with DOX increased [Ca2+]i irrespectively extracellular Ca2+, indicating...
Treatment of a variety cells and tissues with 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator protein kinase C (PKC) results in the inhibition receptor-coupled inositol phospholipid-specific phospholipase (PLC) activity. To determine whether or not targets TPA-activated PKC include one more isozymes PLC, studies were carried out PC12, C6Bu1, NIH 3T3 cells, which contain at least three PLC isozymes, PLC-beta, PLC-gamma, PLC-delta. TPA stimulated phosphorylation serine residues but...
Progesterone-induced calcium ion (Ca2+) signals in the neck region of sperm play a pivotal role promoting motility. Here, we show that long-lasting Ca2+ signal required for motility response to progesterone depends on their pH-dependent fusion with prostasomes, which are small vesicles secreted by prostate. We found prostasome led transfer receptors, cyclic adenosine diphosphoribose (cADPR)-synthesizing enzymes, ryanodine receptors (RyRs), and other signaling tools from prostasomes neck....
Nonalcoholic fatty liver disease (NAFLD), the hepatic manifestation of metabolic syndrome, has emerged as one most common causes chronic in developed countries over last decade. NAFLD comprises a spectrum pathological changes, including steatosis, steatohepatitis, advanced fibrosis, and cirrhosis. Autophagy, homeostatic process for protein organelle turnover, is decreased during development NAFLD. Previously, we have shown that carbon monoxide (CO), reaction product heme oxygenase (HO)...
Glucagon-like peptide-1 (GLP-1) increases intracellular Ca(2+) concentrations ([Ca(2+)](i)), resulting in insulin secretion from pancreatic beta-cells. The molecular mechanism(s) of the GLP-1-mediated regulation [Ca(2+)](i) was investigated.GLP-1-induced changes were measured beta-cells isolated Cd38(+/+) and Cd38(-/-) mice. Calcium-mobilizing second messengers identified by measuring levels nicotinic acid adenine dinucleotide phosphate (NAADP) cyclic ADP-ribose (ADPR), using a enzymatic...
The mechanism by which cAMP modulates the activity of phosphoinositide-specific phospholipase C (PLC) was studied. Elevation inhibited both basal and norepinephrine-stimulated phosphoinositide breakdown in C6Bu1 cells contain at least three PLC isozymes, PLC-beta, PLC-gamma, PLC-delta. Treatment with cAMP-elevating agents (cholera toxin, isobutylmethylxanthine, forskolin, 8-bromo-cAMP) increased serine phosphate but contents PLC-beta PLC-delta were not changed. In addition, cAMP-dependent...
Abstract Carbon monoxide (CO) can confer protection against cellular stress, whereas the potential involvement of autophagy and lysosomal biogenesis remains incompletely understood. We demonstrate here that activation protein kinase R (PKR)-like endoplasmic reticulum (ER) (PERK) with CO increased nuclear translocation transcription factor EB (TFEB). PERK by intracellular Ca 2+ concentration phosphatase activity calcineurin TFEB. Moreover, we found in deficiency TFEB, not only failed to...
The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating factor (M-CSF) and receptor activator nuclear kappaB ligand (RANKL). extracellular matrix (ECM) an important influencing cell fate. To date, little investigation on the relationship between ECM components osteoclast has been documented. In this study, we uncovered a potent anti-osteoclastogenic effect hyaluronan (HA), component present in marrow soft...
We have previously demonstrated that cyclic ADP-ribose (cADPR) is a calcium signaling messenger in interleukin 8 (IL-8)-induced lymphokine-activated killer (LAK) cells. In this study we examined the possibility IL-8 activates CD38 to produce another messenger, nicotinic acid adenine dinucleotide phosphate (NAADP), LAK cells, and showed induced NAADP formation after cADPR production. These messengers were not produced when cells prepared from knock-out mice treated with IL-8, indicating...
CD38 is an enzyme that catalyzes the formation of cyclic ADP ribose and nicotinic acid adenine dinucleotide phosphate, both which are involved in mobilization Ca(2+) from intracellular stores. Recently, has been shown to regulate oxytocin release hypothalamic neurons. Importantly, mutations associated with autism spectrum disorders (ASD) knockout (CD38(-/-)) mice display ASD-like behavioral phenotypes including deficient parental behavior poor social recognition memory. Although ASD learning...
Muscle contraction and insulin induce glucose uptake in skeletal muscle through GLUT4 membrane translocation. Beneficial effects of exercise on homeostasis insulin-resistant individuals are known to be due their distinct mechanism between action muscle. However, the underlying mechanisms not clear. Here we show that muscle, Ca2+ second messengers regulate translocation by treatment; d-myo-inositol 1,4,5-trisphosphate/nicotinic acid adenine dinucleotide phosphate (NAADP) cyclic...
Recent rodent and human studies provide evidence in support of the fact that CD157, well known as bone marrow stromal cell antigen-1 (BST-1) a risk factor Parkinson's disease, also meaningfully acts brain neuroregulator affects social behaviors. It has been shown behaviors are impaired CD157 knockout mice without severe motor dysfunction CD157/BST1 gene single nucleotide polymorphisms associated with autism spectrum disorder humans. However, it is still necessary to determine how this...
The prevalence of metabolic diseases, including type 2 diabetes, obesity, and cardiovascular disease, has rapidly increased, yet the molecular mechanisms underlying syndrome, a primary risk factor, remain incompletely understood. small, gaseous molecule carbon monoxide (CO) well-known anti-inflammatory, antiproliferative, antiapoptotic effects in variety cellular- tissue-injury models, whereas its potential on complex pathways disease unknown. We demonstrate here that CO can alleviate...
CD38 is a type II transmembrane glycoprotein found on both hematopoietic and non-hematopoietic cells. It known for its involvement in the metabolism of cyclic ADP-ribose (cADPR) nicotinic acid adenine dinucleotide phosphate, two nucleotides with calcium mobilizing activity independent inositol trisphosphate. generally believed that an integral protein ectoenzymatic activities mainly plasma membrane. Here we show enzymatically active present intracellularly nuclear envelope rat hepatocytes....
Oxidative stress plays an important role in the pathogenesis of bronchial asthma. An excess production reactive oxygen species (ROS) and defective endogenous antioxidant defense mechanisms may be present Reduced glutathione (GSH) is one most reducing agents against oxidant free radicals. A agent, L-2-oxothiazolidine-4-carboxylic acid (OTC), a prodrug cysteine, increases intracellular GSH. We have used mouse model for asthma to determine effects OTC on allergen-induced inflammation airway...
CD38 is a type II glycoprotein that responsible for the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) nicotinic acid adenine dinucleotide phosphate (NAADP), Ca2+-mobilizing second messengers. The activation hepatic stellate cells (HSCs) critical event in fibrosis because these are main producers extracellular matrix proteins liver. Recent evidence indicates renin-angiotensin system plays major role liver fibrosis. In this study, we showed angiotensin (Ang II) evoked long lasting Ca2+...
Insulin stimulates glucose uptake through the membrane translocation of GLUT4 and GLUT1. Peroxisome proliferator-activated receptor γ (PPARγ) enhances insulin sensitivity. Here, we demonstrate that GLUT1 translocation, uptake, by activating signaling pathway involving nicotinic acid adenine dinucleotide phosphate (NAADP), a calcium mobilizer, in adipocytes. We also PPARγ mediates sensitization enhancing NAADP production upregulation CD38, only enzyme identified for synthesis. produced both...
Significance In natural matings, semen delivers spermatozoa and immunoregulatory fluids to the female reproductive tract. Here, a soluble form of CD38 (sCD38) is shown play an important role in facilitating maternal immune tolerance against fetus by inducing development uterine tolerogenic DCs forkhead box P3 + (Foxp3 ) regulatory T cells. Deficiency sCD38 seminal fluid increased rates loss allogeneic fetuses, this was rescued direct injection recombinant into uterus. Thus, acts as pivotal...
Ca2+ signaling plays a fundamental role in cardiac hypertrophic remodeling, but the underlying mechanisms remain poorly understood. We investigated of Ca2+-mobilizing second messengers, NAADP and cADPR, hypertrophy induced by β-adrenergic stimulation isoproterenol. Isoproterenol an initial transients followed sustained rises. Inhibition cADPR pathway with 8-Br-cADPR abolished only increase, whereas inhibition bafilomycin-A1 both rapid phases isoproterenol-mediated signal, indicating that...
The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in regulation basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents lysosome, which important maintaining homeostasis during environmental stress. Furthermore, oxidative stress critical cause for progression neurodegenerative diseases. Curcumin has anti-oxidative anti-inflammatory activities, expected...