A5005876871- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Genetic Neurodegenerative Diseases
- Cell Adhesion Molecules Research
- Nutrition and Health in Aging
- Cancer Research and Treatments
- Cardiomyopathy and Myosin Studies
- Sirtuins and Resveratrol in Medicine
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Exercise and Physiological Responses
- Cardiac electrophysiology and arrhythmias
- Muscle metabolism and nutrition
- Immunotherapy and Immune Responses
- Muscle activation and electromyography studies
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Spaceflight effects on biology
- Polymer Surface Interaction Studies
- Genetics, Aging, and Longevity in Model Organisms
- Pregnancy and preeclampsia studies
- Thermoregulation and physiological responses
- Connective tissue disorders research
- Diet and metabolism studies
University of Trieste
2019-2025
European Molecular Biology Laboratory
2019
University of Siena
2003-2018
Institut de Myologie
2014
Institute of Molecular Medicine
2013
Centre National de la Recherche Scientifique
2001-2002
National Institutes of Health
2002
Institut Curie
2001-2002
Centro di Riferimento Oncologico
1999-2000
Assembly of specialized membrane domains, both the plasma and ER, is necessary for physiological activity striated muscle cells. The mechanisms that mediate structural organization sarcoplasmic reticulum with respect to myofibrils are, however, not known. We report here ank1.5, a small splice variant ank1 gene localized on membrane, capable interacting sequence 25 aa located at COOH terminus obscurin. Obscurin giant sarcomeric protein ∼800 kD binds titin has been proposed interactions...
Few days of unloading are sufficient to induce a decline skeletal muscle mass and function; notably, contractile force is lost at faster rate than mass. The reasons behind this disproportionate loss still poorly understood. We provide strong evidence two mechanisms only hypothesized until now for the rapid in 10 bed rest. Our results show that an initial neuromuscular junction instability, accompanied by alterations innervation status impairment single fibre sarcoplasmic reticulum function...
Junctophilins (JPs) anchor the endo/sarcoplasmic reticulum to plasma membrane, thus contributing assembly of junctional membrane complexes in striated muscles and neurons. Recent studies have shown that JPs may be also involved regulating Ca2+ homeostasis. Here, we report skeletal muscle, JP1 JP2 are part a complex that, addition ryanodine receptor 1 (RyR1), includes caveolin 3 dihydropyridine (DHPR). The interaction between DHPR was mediated by region encompassing amino acids 230–369...
Obscurin is a large myofibrillar protein that contains several interacting modules, one of which mediates binding to muscle-specific ankyrins. Interaction between obscurin and the ankyrin sAnk1.5 regulates organization sarcoplasmic reticulum in striated muscles. Additional isoforms, ankB ankG, are localized at subsarcolemma level, they contribute dystrophin β-dystroglycan costameres. In this paper, we report mice deficient for obscurin, was displaced from its localization M band, whereas...
Abstract Short‐term unloading experienced following injury or hospitalisation induces muscle atrophy and weakness. The effects of exercise have been scarcely investigated. We investigated the functional molecular adaptations to a resistance training (RT) programme short‐term unloading. Eleven males (22.09 ± 2.91 years) underwent 10 days unilateral lower limb suspension (ULLS) followed by 21 knee extensor RT (three times/week). Data collection occurred at Baseline (LS0), after ULLS (LS10)...
Follicle-associated epithelium (FAE) covering the lymphoid follicles of Peyer’s patches (PPs) plays a central role in mucosal immunity. Here, we investigated FAE-derived intrafollicular epithelial bodies (IFEBs) that apparently detach from FAE and sink deep into tissue PPs. Analysis rabbit PP was carried out by variety microscopy immunohistochemistry techniques using panel specific antibodies to determine nature IFEBs. IFEBs displayed typical features FAE, with cytokeratin (CK)+ cells...
EMILIN (elastin microfibril interface located protein) is an extracellular matrix glycoprotein abundantly expressed in elastin-rich tissues such as blood vessels, skin, heart, and lung. It occurs associated with elastic fibers at the between amorphous elastin microfibrils. Avian was extracted from 19-day-old embryonic chick aortas vessels purified by ion-exchange chromatography gel filtration. Tryptic peptides were generated sequenced, degenerate inosine-containing oligonucleotide primers...
Skeletal muscle aging is accompanied by mass reduction and functional decline, as a result of multiple factors, such protein expression, morphology organelles, metabolic equilibria, neural communication. muscles are formed fibers that express different Myosin Heavy Chains (MyHCs) have properties blood supply, with the purpose to adapt their contraction need. The fine interplay between composing its architectural organization determine properties. Immunohistochemical histochemical analyses...
Muscle-specific ankyrins 1 (sAnk1) are a group of small ankyrin isoforms, which sAnk1.5 is the most abundant. sAnk1 localized in sarcoplasmic reticulum (SR) membrane from where they interact with obscurin, myofibrillar protein. This interaction appears to contribute stabilize SR close myofibrils. Here we report structural and functional characterization skeletal muscles knockout mice (KO). Deletion did not change expression localization proteins 4- 6-mo-old KO mice. Structurally, main...
The primary structure of humanElastin microfibrilinterface-located protein (EMILIN), an elastic fiber-associated glycoprotein, consists a globular C1q domain (gC1q) at the C terminus, short collagenous stalk, long region with high potential for forming coiled-coil α helices, and cysteine-rich N-terminal sequence. It is not known whether EMILIN gC1q involved in assembly process supramolecular organization as shown similar collagen X. By employing yeast two-hybrid system domains interacted...
The sarcoplasmic reticulum (SR) of skeletal muscle cells is a complex network tubules and cisternae that share common lumen delimited by single continuous membrane. SR contains longitudinal junctional domains characterized distinctive patterns protein localization, but how proteins reach and/or are retained at these sites not known. Here, we report the organization slow process temporally distinct localization. In contrast, rapidly synchronously assembled into clusters which, however, merged...
In the context of increasing number obese individuals, a major problem is represented by obesity and malnutrition in children. This condition mainly ascribable to unbalanced diets characterized high intakes fat sugar. Childhood are not only associated with concurrent pathologies but potentially compromise adult life. Considering strict correlation among systemic metabolism, obesity, skeletal muscle health, we wanted study impact juvenile on muscle. To this aim, 3-week-old C56BL/6 female male...
A new biorelevant polymer brush showing a polybenzofulvene backbone was synthesized by "grafting through" approach based on click chemistry and spontaneous polymerization reactions. The easy of the relatively complex monomer (6-MOEG-9-TM-BF3k) suggests existence particularly efficient recognition process capable pre-organizing molecules for polymerization. 13C-NMR spectroscopy as well UV-vis fluorescence suggested poly-6-MOEG-9-TM-BF3k features vinyl (1,2) π-stacked polymer. derivative found...
We recently showed that the treatment with Resveratrol (RES) contrasts effects of ageing on skeletal muscle (SKM), reduces appearance tubular aggregates (TAs), and improves fatigue resistance. Since resistance depends SKM capillary network, RES has been described to improve vascularisation, we analysed capillarization in naturally C57BL/6J male mice, fed 0.04% diet for 6 months, which a better previous work. Our data show an inverse correlation between number capillaries per fibre (CAF) TAs...
Inactivity and unloading induce skeletal muscle atrophy, loss of strength detrimental metabolic effects. Bed rest is a model to study the impact inactivity on musculoskeletal system. It not only provides information for bed-ridden patients care, but it also ground-based spaceflight analogue used mimic challenges long space missions human body. In both cases, would be desirable develop panel biomarkers monitor atrophy in minimally invasive way at point care limit onset personalized fashion.