A5080174536- Immune Cell Function and Interaction
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- T-cell and B-cell Immunology
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Urinary Tract Infections Management
- Bacterial biofilms and quorum sensing
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- Clostridium difficile and Clostridium perfringens research
- Sepsis Diagnosis and Treatment
- Neonatal and Maternal Infections
- Cytomegalovirus and herpesvirus research
- Gut microbiota and health
- Microbial infections and disease research
- Cystic Fibrosis Research Advances
- Diabetes and associated disorders
- Moyamoya disease diagnosis and treatment
- Reliability and Maintenance Optimization
- HIV-related health complications and treatments
Istituto Giannina Gaslini
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2025
University of Genoa
1990-2020
Amsterdam UMC Location University of Amsterdam
2011
Abstract Background Guidelines about febrile neutropenia in paediatric patients are not homogeneous; the best empiric treatment of this condition should be driven by local epidemiology. The Weighted-Incidence Syndromic Combination Antibiogram (WISCA) addresses need for disease-specific susceptibility evidence that could guide antibiotic prescriptions based on outcome estimates regimens obtained as a weighted average pathogen susceptibilities. This study developed WISCA model to inform...
Abstract Aims The purpose of this work is to find the gut microbial fingerprinting pediatric patients with type 1 diabetes. Methods microbiome 31 children diabetes at onset and 25 healthy was determined using multiple polymorphic regions 16S ribosomal RNA. We performed machine-learning analyses metagenome functional analysis identify significant taxa their metabolic pathways content. Results Compared controls, showed a significantly higher relative abundance following most important taxa:...
In last years the diffusion of carbapenem resistance in Gram-negative bacteria (CR-GNB) is increasing worldwide, mainly due to expression carbapenemases. Cefiderocol has molecular characteristics that ideally confers activity against all CR-GNB, but resistant strains have already been identified. We describe cefiderocol susceptibility profile among multi-drug isolated from pediatric patients.
Background: Urinary tract infections (UTIs) are among the most common bacterial in children, and antibiotic susceptibility youngest patients remains poorly understood. This study aimed to describe distribution of uropathogens their susceptibility, focusing on oral formulations. Methods: Data from first microbiological isolation, between January 2007 December 2023, at Istituto Gaslini, young infants (aged <6 months), were analyzed. Results: We isolated 2473 infants’ pathogen, with a median...
Bloodstream infections play an important role in neonatal morbidity and mortality. In this study, we retrospectively analyzed etiology antibiotic resistance profiles of bacteria isolated from blood or Cerebro Spinal Fluid (CSF) cultures to evaluate the appropriateness initial empirical therapy sepsis.microbiological data patients admitted Neonatal Intensive Care Unit (NICU), January 2005 October 2018, were anonymously extracted Laboratory Microbiology database. According sepsis definition...
In a prospective study of acute pharyngitis in Italian children, 69 (38.3%) 180 isolates Streptococcus pyogenes were resistant to macrolides. S. was eradicated 12 (63.1%) 19 patients with erythromycin-resistant treated clarithromycin and 22 (88%) 25 erythromycin-susceptible strains. The constitutive-resistant phenotype correlated failure macrolide treatment.
The surface marker PROM1 is considered one of the most important markers tumor-initiating cells, and its expression believed to be an adverse prognostic factor in gliomas other malignancies. To date, our knowledge, no specific studies medulloblastoma series have been performed. aims study were evaluate profile gene assess possible role as a factor. was evaluated by quantitative– polymerase chain reaction on 45 samples using dye-labeled probe systems. A significantly higher found both...
BACKGROUND: Moyamoya disease (MMD) is a chronic, occlusive cerebrovascular characterized by bilateral steno-occlusive changes at the terminal portion of internal carotid arteries and an abnormal vascular network base brain determining stroke in children. Patients with similar vasculopathy associated conditions are affected moyamoya syndrome (MMS). Most studies focused on MMD were carried out East-Asian population. Ring Finger 213 (RNF213) has been identified as strongest susceptibility gene...
Abstract Several studies demonstrated a relevant role of polymorphisms located within the HLA-B and -C loci Killer Immunoglobulin Receptors (KIRs) 3DL1 3DS1 in controlling HIV-1 replication. KIRs are regulatory receptors expressed at surface NK CD8+ T-cells that specifically bind HLA-A -B alleles belonging to Bw4 supratype all expressing C1 or C2 supratype. We here disclose novel signature associated with Elite Controller but not long-term nonprogressor status concerning 2DS activating...
HLA-C expression is associated with a differential ability to control HIV-1 infection. Higher levels may lead better of infection through both higher efficiency antigen presentation cytotoxic T lymphocytes and the triggering activating killer immunoglobulin-like receptors on NK cells, whereas lower provide poor rapid progression AIDS. We characterized relative amounts heterotrimers (heavy chain/β2 microglobulin [β2m]/peptide) free heavy chains peripheral blood mononuclear cells (PBMCs) from...
Molecular and functional characterization of the natural cytotoxicity receptor (NCR) NKp44 in species other than Homo sapiens has been elusive, so far. Here, we provide complete phenotypic, molecular for triggering on Pan troglodytes NK cells, closest human relative, analysis NKp44-genomic locus transcription Macaca fascicularis. Similar to H. sapiens, expression is detectable chimpanzee cells only upon activation. However, basal 5-fold higher chimpanzees with lower differential increases...
Abstract HIV‐1 infection in humans results an early and progressive NK cell dysfunction accumulation of “anergic” CD56 − CD16 + subset, which is characterised by low natural cytotoxicity receptor expression cytokine producing capacity. In contrast to humans, chimpanzee cells do not display a distinguishable bright dim subset but, as shown here, could be subdivided into functionally different CD8 subsets. The expressed significantly higher levels triggering receptors including NKp46 and, upon...
Quantitative real-time PCR (qPCR) has been improved and optimized over the past decade for a wide range of applications. Design primers probes is one crucial steps to obtain high system efficiency qPCR since design pitfalls influence negatively amplification performances. We report results some experiments. First, we demonstrate utility optimal primer concentration in by constructing suboptimal primers, instance with hairpin primer-dimers secondarystructures, quantifying decrease...
Monitoring antibiotic use in the pediatric population is a challenge, especially when determining relationship between specific pathogens, infections, and use. We retrospectively analyzed consumption of anti-methicillin-resistant Staphylococcus aureus (MRSA) drugs from 2017 to 2021 at Istituto Giannina Gaslini by means defined daily dose (DDD) adopted for adults World Health Organization. observed statistically significant increase daptomycin ceftaroline, combined with decrease vancomycin....
Natural killer (NK) cells are lymphoid effectors that involved in the innate immune surveillance against infected and/or tumor cells. Their function is under fine-tuning control of cell surface receptors display either inhibitory or activating and healthy condition, mediate self-tolerance. It known characterized by clonal stochastic distribution extremely sensible to any modification, down-regulation loss MHC class I expression induced autologous upon viral infection cancer transformation....
Background: To analyze the genetic characteristics of Acinetobacter-calcoaceticus-baumannii complex strains isolated in suspected outbreak a Intensive Care Unit pediatric hospital order to promptly stop dissemination this dangerous strain.Methods: This study described use whole genome obtained by Next Generation Sequencing define clonality 13 and their Resistoma. was required because Acinetobacter baumannii is known be resistant desiccation disinfectants difficult treat eradicate. Thus,...
It is not yet clear whether immature NK (iNK) cells are bystanders to or rather participate in immune responses pathogens that may colocalize areas of NK-cell maturation such as bone marrow lymph nodes. Mycobacteria, including Bacillus Calmette-Guerin (BCG), have been shown interact with peripheral and vivo iNK-cell development. We studied infection BCG human cord blood CD34(+) Lin(-)-derived cultures containing myelomonocytes iNK vitro. Increased DNAM-1 expression, transient natural...